##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546021_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	227581
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.977810098382555	31.0	28.0	33.0	23.0	33.0
2	28.77121112922432	31.0	28.0	33.0	16.0	33.0
3	28.209881316981647	31.0	27.0	33.0	16.0	34.0
4	32.25143575254525	35.0	32.0	35.0	26.0	37.0
5	33.63762352744737	35.0	33.0	35.0	28.0	37.0
6	33.40437470614858	35.0	33.0	37.0	28.0	37.0
7	34.428968147604586	35.0	35.0	37.0	32.0	37.0
8	34.77950268256137	35.0	35.0	37.0	32.0	37.0
9	36.553341447660394	39.0	37.0	39.0	32.0	39.0
10	36.25764013691828	38.0	35.0	39.0	31.0	39.0
11	36.3199651992038	38.0	35.0	39.0	31.0	39.0
12	35.11223696178504	37.0	34.0	39.0	29.0	39.0
13	35.39177699368577	37.0	35.0	39.0	30.0	39.0
14	36.10138807721207	38.0	34.0	40.0	30.0	41.0
15	36.6496631968398	38.0	35.0	40.0	31.0	41.0
16	36.219842605489916	38.0	34.0	40.0	31.0	41.0
17	35.810199445472165	37.0	34.0	40.0	30.0	41.0
18	35.45126350618022	37.0	34.0	39.0	29.0	40.0
19	34.9390546662507	37.0	33.0	39.0	27.0	41.0
20	35.43089273709141	36.0	34.0	39.0	30.0	41.0
21	35.628097248891606	36.0	34.0	39.0	30.0	41.0
22	35.98400130063582	37.0	34.0	40.0	31.0	41.0
23	36.29088104894521	37.0	35.0	40.0	31.0	41.0
24	35.986211502717715	37.0	35.0	40.0	31.0	41.0
25	35.58815103194028	36.0	34.0	40.0	30.0	41.0
26	35.71803885210101	37.0	34.0	40.0	30.0	41.0
27	36.02418040170313	37.0	35.0	40.0	31.0	41.0
28	35.94169548424517	37.0	35.0	40.0	31.0	41.0
29	36.095473699474034	37.0	35.0	40.0	31.0	41.0
30	35.741314081579745	37.0	35.0	40.0	30.0	41.0
31	35.10325554417987	36.0	34.0	40.0	29.0	41.0
32	35.0724884766303	37.0	34.0	40.0	29.0	41.0
33	34.79783901116526	37.0	34.0	40.0	26.0	41.0
34	34.51069289615565	37.0	34.0	40.0	23.0	41.0
35	34.27789666096906	37.0	34.0	40.0	22.0	41.0
36	33.87619353109442	36.0	33.0	40.0	21.0	41.0
37	33.8090833593314	36.0	33.0	40.0	20.0	41.0
38	33.64695646824647	36.0	33.0	40.0	19.0	41.0
39	33.442427091892554	36.0	33.0	40.0	18.0	41.0
40	33.263422693458594	36.0	33.0	40.0	18.0	40.0
41	32.98643999279378	35.0	33.0	39.0	18.0	40.0
42	33.16063291751069	36.0	33.0	40.0	18.0	40.0
43	32.93003370228622	35.0	33.0	39.0	17.0	40.0
44	32.789283815432746	35.0	32.0	39.0	16.0	40.0
45	32.70911016297494	35.0	32.0	39.0	17.0	40.0
46	32.54379759294493	35.0	32.0	39.0	17.0	40.0
47	32.49560376305579	35.0	32.0	39.0	17.0	40.0
48	32.49084501781783	35.0	32.0	39.0	17.0	40.0
49	32.485963239461995	35.0	32.0	39.0	17.0	40.0
50	32.66101739600406	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	5.0
14	8.0
15	32.0
16	55.0
17	124.0
18	252.0
19	415.0
20	598.0
21	913.0
22	1309.0
23	1892.0
24	2609.0
25	4038.0
26	5682.0
27	6693.0
28	6395.0
29	6425.0
30	6960.0
31	8572.0
32	11513.0
33	17611.0
34	27251.0
35	21526.0
36	23130.0
37	32043.0
38	34348.0
39	7177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.22731247336113	22.624911569946523	28.017277364982142	21.130498591710204
2	37.435462538612626	20.37164789679279	29.39217245727895	12.800717107315638
3	28.864448262376914	19.122422346329436	38.711052328621456	13.30207706267219
4	25.622086202275234	25.165984858138422	34.92558693388288	14.286342005703464
5	21.411717146861996	28.546319771861445	35.76748498336856	14.274478097907997
6	19.208545528844674	37.53960128481727	32.931132212267286	10.32072097407077
7	77.73408149186444	4.824216432830509	14.928750642628339	2.5129514326767173
8	78.8251216050549	3.648371349102078	14.814945008590346	2.7115620372526705
9	75.08491482153606	5.010963129610996	16.020230159811234	3.883891889041704
10	44.49229065695291	22.072580751468706	21.42226284268019	12.012865748898193
11	37.95571686564344	21.180590646846618	27.552827344989257	13.310865142520683
12	33.489614686639044	21.08128534455864	31.417824862356696	14.011275106445616
13	20.685382347384007	32.08527952684978	32.04880899547853	15.18052913028768
14	16.76150469503166	32.64068617327457	34.9295415698147	15.668267561879068
15	16.56113647448601	21.648995302771322	46.24727020269706	15.54259802004561
16	18.567455103897075	18.499786889063675	44.53007940030143	18.402678606737823
17	19.25204652409472	18.516923644768234	34.263405117298895	27.967624713838152
18	22.037428432074734	21.186742302740562	37.856411563355465	18.91941770182924
19	26.454317363927572	22.855598665969477	31.848001370940455	18.842082599162495
20	28.636397590308505	21.623070467218263	30.38258905620416	19.357942886269065
21	22.833628466348248	25.10227127923684	33.45929581116174	18.60480444325317
22	22.188583405468822	22.030837372188365	32.13756860194832	23.6430106203945
23	20.057474042209147	25.83607594658605	32.10900734244071	21.997442668764087
24	19.976623707603007	22.16309797390819	40.098690136698586	17.76158818179022
25	18.690488221775983	23.05948211845453	38.20529833334066	20.04473132642883
26	18.150900119078482	28.568729375475105	33.71810476269987	19.562265742746536
27	18.613153119109242	27.976412793686645	34.04502133306383	19.365412754140284
28	17.38677657625197	24.81929510811535	40.07012887719098	17.7237994384417
29	18.233508069654324	22.836704294295217	39.008089427500536	19.921698208549923
30	20.249054182906306	25.744679916161715	35.56931378278503	18.436952118146944
31	26.87658460064768	23.226455635575903	31.567222219781087	18.329737543995325
32	27.445612770837634	23.857000364705314	31.18406193838677	17.51332492607028
33	26.2732829190486	24.607502383766658	29.919017844196134	19.200196852988608
34	20.428770415808	25.786423295442063	31.733316928917617	22.051489359832324
35	21.416111186786242	25.30879115567644	32.9030103567521	20.372087300785218
36	27.065967721382716	24.61497225163788	29.078877410680153	19.24018261629925
37	21.768073784718407	29.51169034321846	29.354383713930428	19.36585215813271
38	22.40345195776449	29.614950281438258	27.52294787350438	20.458649887292875
39	21.486855229566615	29.409748616975932	28.39516479846736	20.70823135499009
40	24.191826206932916	25.099634855282293	28.46986347717955	22.238675460605233
41	19.72132998800427	24.348693432228526	29.27573039928641	26.654246180480794
42	22.472877788567587	24.66023086285762	26.954359107306846	25.912532241267943
43	22.209674797105205	25.31450340757796	28.285753204353615	24.190068590963218
44	21.19289395863451	27.154287923860075	30.4348781313027	21.217939986202712
45	19.812726018428602	31.422218902280946	26.9161309599659	21.848924119324547
46	22.04841353188535	28.96111714071034	28.652216134035797	20.338253193368516
47	21.488612845536316	26.298328946616813	29.358338349862244	22.85471985798463
48	22.060277439680817	24.172053027273808	32.09230999072857	21.6753595423168
49	21.29747210883158	24.20984177062233	32.84940306967629	21.643283050869798
50	19.951138276042375	29.164561189202963	29.80037876624147	21.08392176851319
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	181.0
2	292.0
3	3065.5
4	5839.0
5	4154.5
6	2470.0
7	2482.0
8	2494.0
9	2543.5
10	2593.0
11	2549.0
12	2505.0
13	2416.0
14	2327.0
15	2128.0
16	1929.0
17	1779.0
18	1629.0
19	1575.0
20	1521.0
21	1441.0
22	1361.0
23	1407.0
24	1453.0
25	1434.5
26	1416.0
27	1674.0
28	1932.0
29	2242.5
30	2553.0
31	3207.5
32	3862.0
33	4029.0
34	4196.0
35	4988.5
36	5781.0
37	6226.0
38	6671.0
39	8119.5
40	9568.0
41	12250.0
42	14932.0
43	16842.5
44	18753.0
45	18680.5
46	18608.0
47	18084.0
48	17560.0
49	17162.0
50	16764.0
51	15774.5
52	14785.0
53	13918.0
54	13051.0
55	11585.5
56	10120.0
57	9735.0
58	9350.0
59	8627.0
60	7904.0
61	7167.0
62	6430.0
63	5857.5
64	5285.0
65	4606.0
66	3927.0
67	3319.0
68	2711.0
69	2301.0
70	1891.0
71	1591.5
72	1292.0
73	1027.5
74	763.0
75	604.5
76	446.0
77	365.0
78	284.0
79	199.5
80	115.0
81	92.5
82	70.0
83	51.5
84	33.0
85	27.0
86	21.0
87	18.0
88	15.0
89	8.5
90	2.0
91	3.5
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	227581.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.9179061520953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46908562243404	30.673035095196877
2	10.25769659987919	8.80477720020564
3	4.719830454680413	6.076957215233258
4	2.9895672294288085	5.132238631520206
5	1.9647190113951656	4.2160813073147585
6	1.483521546384364	3.820178310140126
7	1.123135359823083	3.3741832578290802
8	0.9265610762442027	3.181284905154648
9	0.7596777000808822	2.934339861411981
>10	4.277538316627932	29.362732389786494
>50	0.023547961053720066	0.5861649258945166
>100	0.004095297574560011	0.22277782415931033
>500	0.0	0.0
>1k	0.0010238243936400028	1.615249076153106
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3676	1.615249076153106	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3940399242467515E-4	0.0	0.0	0.02372781559093246	0.0
2	4.3940399242467515E-4	0.0	0.0	0.09666887833342853	0.0
3	4.3940399242467515E-4	0.0	0.0	0.12786656179558048	0.0
4	4.3940399242467515E-4	0.0	0.0	0.1928983526744324	0.0
5	4.3940399242467515E-4	0.0	0.0	0.3945847851973583	0.0
6	4.3940399242467515E-4	0.0	0.0	0.5334364468035556	0.0
7	4.3940399242467515E-4	0.0	0.0	0.6446056568869984	0.0
8	4.3940399242467515E-4	0.0	0.0	0.9227483840918178	0.0
9	4.3940399242467515E-4	0.0	0.0	1.0541301778267957	0.0
10	4.3940399242467515E-4	0.0	0.0	1.304151049516436	0.0
11	4.3940399242467515E-4	0.0	0.0	1.4768368185393332	0.0
12	4.3940399242467515E-4	0.0	0.0	1.6692957672213409	0.0
13	4.3940399242467515E-4	0.0	0.0	1.7488278898502072	0.0
14	4.3940399242467515E-4	0.0	0.0	1.7795861693199344	0.0
15	4.3940399242467515E-4	0.0	0.0	1.82616299251695	0.0
16	4.3940399242467515E-4	0.0	0.0	1.9193166389109813	0.0
17	4.3940399242467515E-4	0.0	0.0	2.0309252529868487	0.0
18	4.3940399242467515E-4	0.0	0.0	2.1895500942521564	0.0
19	4.3940399242467515E-4	0.0	0.0	2.249309037221912	0.0
20	4.3940399242467515E-4	0.0	0.0	2.3231289079492576	0.0
21	4.3940399242467515E-4	0.0	0.0	2.4167219583357133	0.0
22	4.3940399242467515E-4	0.0	0.0	2.501526928873676	0.0
23	4.3940399242467515E-4	0.0	0.0	2.595998787244981	0.0
24	4.3940399242467515E-4	0.0	0.0	2.6711368699496005	0.0
25	4.3940399242467515E-4	0.0	0.0	2.737926276798151	0.0
26	4.3940399242467515E-4	0.0	0.0	2.7981246237603314	0.0
27	4.3940399242467515E-4	0.0	0.0	2.8649140306088823	0.0
28	4.3940399242467515E-4	0.0	0.0	2.930385225480159	0.0
29	4.3940399242467515E-4	0.0	0.0	3.0011292682605313	0.0
30	4.3940399242467515E-4	0.0	0.0	3.104828610472755	0.0
31	4.3940399242467515E-4	0.0	0.0	3.193588216942539	0.0
32	4.3940399242467515E-4	0.0	0.0	3.269605107632008	0.0
33	4.3940399242467515E-4	0.0	0.0	3.3460614023139015	0.0
34	4.3940399242467515E-4	0.0	0.0	3.427790544904891	0.0
35	4.3940399242467515E-4	0.0	0.0	3.5473084308444025	0.0
36	4.3940399242467515E-4	0.0	0.0	3.632113401382365	0.0
37	4.3940399242467515E-4	0.0	0.0	3.724388239791547	0.0
38	4.3940399242467515E-4	0.0	0.0	3.824132946071948	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.843163E-4	44.0	28
GACGTTG	20	7.843163E-4	44.0	1
ATTACGG	20	7.843163E-4	44.0	2
TTCGTAG	20	7.843163E-4	44.0	1
CACGACG	20	7.843163E-4	44.0	26
AATTCGC	20	7.843163E-4	44.0	13
TTCGGGC	20	7.843163E-4	44.0	4
ACGTAGG	20	7.843163E-4	44.0	2
ATAGTTA	20	7.843163E-4	44.0	37
ACTCATG	25	4.431557E-5	44.0	22
CGTTTTT	2560	0.0	42.882812	1
AATCCTA	105	0.0	41.90476	13
TAGGGGT	90	0.0	41.555557	5
GGGCGAT	310	0.0	41.161293	7
TAACGGG	70	0.0	40.857143	3
CGAGGGC	60	3.6379788E-12	40.333332	4
AGGGCGA	170	0.0	40.11765	6
ATCCTAG	110	0.0	40.0	14
TAATCCT	105	0.0	39.80952	12
CTTGTAG	45	2.3366738E-8	39.11111	1
>>END_MODULE