FastQCFastQC Report
Thu 26 May 2016
SRR1546020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546020_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences229086
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37941.6561465999668248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG302.5188128E-644.0000042
CGGGTAG207.8432716E-444.06
CGAACGG207.8432716E-444.02
GACCGAT454.765752E-1044.09
ACGGGAC351.4392936E-744.05
ATCGTAG207.8432716E-444.01
GTATGAT207.8432716E-444.09
TCGTAAG254.4316494E-544.01
CGTAAGG502.7284841E-1144.02
TATACGG207.8432716E-444.02
CGTTTTT24200.043.181821
GGGCGAT2550.041.4117667
TGGGCGA850.041.4117666
AACGGGA557.6397555E-1140.04
CTTTAGG501.3369572E-939.62
AGGGCGA1450.039.4482736
GAACGGG950.039.3684233
GCGCGAC452.3368557E-839.111119
CGACCCG452.3368557E-839.1111132
CGCGACC452.3368557E-839.1111110
Produced by FastQC (version 0.11.5)