##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546020_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	229086
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96725247287045	31.0	28.0	33.0	23.0	33.0
2	28.73457129636905	31.0	28.0	33.0	16.0	33.0
3	28.19815702399972	31.0	27.0	33.0	16.0	34.0
4	32.28093379778773	35.0	32.0	35.0	26.0	37.0
5	33.68448530246283	35.0	33.0	35.0	28.0	37.0
6	33.5225155618414	35.0	33.0	37.0	28.0	37.0
7	34.51401657019635	35.0	35.0	37.0	32.0	37.0
8	34.82872807591909	35.0	35.0	37.0	32.0	37.0
9	36.657137494216144	39.0	37.0	39.0	32.0	39.0
10	36.2425813886488	38.0	35.0	39.0	31.0	39.0
11	36.37178177627616	38.0	35.0	39.0	32.0	39.0
12	35.069524108849954	37.0	34.0	39.0	29.0	39.0
13	35.39206673476336	37.0	35.0	39.0	30.0	39.0
14	36.11035593619863	38.0	35.0	40.0	30.0	41.0
15	36.68592144434841	38.0	35.0	40.0	31.0	41.0
16	36.26229887465843	38.0	34.0	40.0	31.0	41.0
17	35.85487982678994	37.0	34.0	40.0	30.0	41.0
18	35.54659385558262	37.0	34.0	39.0	29.0	40.0
19	35.02236278078975	37.0	33.0	39.0	27.0	41.0
20	35.398920056223425	36.0	34.0	39.0	30.0	41.0
21	35.51063792636826	36.0	34.0	39.0	30.0	40.0
22	35.94856080249339	37.0	34.0	40.0	31.0	41.0
23	36.32501331377736	37.0	35.0	40.0	32.0	41.0
24	35.87427429000463	36.0	35.0	40.0	31.0	41.0
25	35.52793274141589	36.0	34.0	40.0	30.0	41.0
26	35.70748103332373	37.0	34.0	40.0	30.0	41.0
27	36.04510096644928	37.0	35.0	40.0	31.0	41.0
28	35.958989200562236	37.0	35.0	40.0	31.0	41.0
29	36.13431200509852	37.0	35.0	40.0	31.0	41.0
30	35.793514226098495	37.0	35.0	40.0	30.0	41.0
31	35.14333481749212	36.0	34.0	40.0	29.0	41.0
32	35.14635115196913	37.0	34.0	40.0	29.0	41.0
33	34.88681106658635	37.0	34.0	40.0	27.0	41.0
34	34.54843159337541	37.0	34.0	40.0	24.0	41.0
35	34.34705743694508	37.0	34.0	40.0	23.0	41.0
36	33.94345355019512	36.0	33.0	40.0	21.0	41.0
37	33.85395004496128	36.0	33.0	40.0	20.0	41.0
38	33.724649258357125	36.0	33.0	40.0	20.0	41.0
39	33.480566250229174	36.0	33.0	40.0	18.0	41.0
40	33.28463546441075	36.0	33.0	40.0	18.0	40.0
41	32.98485285002139	35.0	33.0	39.0	18.0	40.0
42	33.136577529835954	35.0	33.0	40.0	18.0	40.0
43	32.942650358380696	35.0	33.0	39.0	17.0	40.0
44	32.78204691687838	35.0	32.0	39.0	16.0	40.0
45	32.70749849401535	35.0	32.0	39.0	17.0	40.0
46	32.50512471298988	35.0	32.0	39.0	17.0	40.0
47	32.475454632758	35.0	32.0	39.0	17.0	40.0
48	32.49241769466489	35.0	32.0	38.0	17.0	40.0
49	32.556157949416374	35.0	32.0	39.0	18.0	40.0
50	32.74357664807103	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	9.0
14	4.0
15	30.0
16	57.0
17	104.0
18	239.0
19	403.0
20	619.0
21	904.0
22	1294.0
23	1818.0
24	2680.0
25	3884.0
26	5654.0
27	6565.0
28	6463.0
29	6388.0
30	7008.0
31	8723.0
32	11538.0
33	17723.0
34	27364.0
35	21847.0
36	23735.0
37	32752.0
38	34868.0
39	6411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.323424390840117	22.390281379045422	28.016116218363408	21.270178011751046
2	37.49596221506334	20.38710353317095	29.436543481487305	12.68039077027841
3	29.12836227442969	19.143902289969706	38.689837004443746	13.037898431156858
4	25.520110351571024	25.21847690387016	34.74721283710048	14.514199907458334
5	21.46966641348664	28.61283535440839	35.73592449996944	14.18157373213553
6	19.22902316160743	37.2873069502283	33.08975668526231	10.393913202901967
7	77.52285168015504	4.823079542180666	15.033655483093685	2.6204132945705982
8	78.63946290912584	3.69162672533459	14.897462088473324	2.7714482770662547
9	75.08708519944474	5.045266843019652	16.07213011707394	3.795517840461661
10	44.427420270116905	21.88261177025222	21.42776075360345	12.262207206027432
11	38.021965550055434	21.29680556646849	27.418960565027984	13.262268318448095
12	33.34075412727098	20.76949267960504	31.56325572055909	14.326497472564888
13	20.76600054128144	31.905485276271794	32.170887788865315	15.15762639358145
14	16.960006285848984	32.44633019913919	35.05408449228674	15.53957902272509
15	16.460193988283876	21.39502195681971	46.42361383934418	15.721170215552238
16	18.442419004216756	18.374322306906578	44.7530621687925	18.43019652008416
17	19.295810307046263	18.39964030975267	34.297163510646655	28.007385872554412
18	22.218730083898624	21.178945898047022	37.73255458648717	18.869769431567185
19	26.58914119588277	22.897950987838627	31.743100844224443	18.769806972054162
20	28.615454458151085	21.2645032869752	30.502955222056343	19.61708703281737
21	22.76219411050872	25.031647503557615	33.49528124809024	18.71087713784343
22	22.24841325964921	22.06332992849847	32.11937874859223	23.568878063260083
23	19.950149725430624	25.845752250246633	32.284818801672735	21.919279222650008
24	20.196345477244353	22.08864793134456	40.149550823708125	17.565455767702957
25	18.81258566651825	22.79275032084021	38.34455182769789	20.050112184943647
26	18.181818181818183	28.580969592205545	33.64151454039094	19.595697685585325
27	18.695162515387235	27.74940415389854	34.164898771640345	19.390534559073885
28	17.438865753472495	24.62088473324428	40.096295714273246	17.843953799009977
29	18.11939620928385	22.840330705499245	39.24421396331509	19.79605912190182
30	20.285395004496127	25.699082440655474	35.49802257667426	18.517499978174136
31	26.777716665357115	23.218791196319284	31.479880918083165	18.523611220240433
32	27.195463712317647	24.021983010747057	31.327972901006607	17.454580375928693
33	26.206315532158232	24.651004426285326	30.19957570519368	18.94310433636276
34	20.552107068961	25.680312197166128	31.81774530089137	21.9498354329815
35	21.50371476214173	25.20625441973757	32.88983176623626	20.400199051884446
36	27.148756362239507	24.488183476947523	28.95637446199244	19.40668569882053
37	21.662170538575033	29.349676540687774	29.471901382013744	19.51625153872345
38	22.39726565569262	29.6054756728914	27.428563945417878	20.568694725998096
39	21.574430563194607	29.12094148049204	28.376679500275003	20.927948456038344
40	24.123691539421877	24.98843229180308	28.63989942641628	22.247976742358766
41	19.746732668080984	24.239368621391094	29.17070445160333	26.843194258924598
42	22.39901172485442	24.865770933186663	26.956252237151112	25.7789651048078
43	22.19690421937613	25.46336310381254	27.98643304261282	24.35329963419851
44	21.231764490191456	27.310267759705965	30.367634862016885	21.090332888085698
45	19.827488366814208	31.161223296054757	26.989864068515757	22.02142426861528
46	21.930628672201706	28.797482168268683	28.80272037575408	20.469168783775526
47	21.630304776372192	26.03301816784963	29.46709969181879	22.869577363959387
48	22.123569314580553	24.27778214295068	31.927747658084737	21.670900884384032
49	21.314702775376933	24.18043878718036	32.88633962791266	21.618518809530045
50	19.86546537108335	29.322175951389433	29.846869734510186	20.965488943017032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	187.5
2	313.0
3	3152.5
4	5992.0
5	4187.0
6	2382.0
7	2407.5
8	2433.0
9	2597.5
10	2762.0
11	2657.5
12	2553.0
13	2428.5
14	2304.0
15	2184.5
16	2065.0
17	1867.0
18	1669.0
19	1576.0
20	1483.0
21	1389.5
22	1296.0
23	1370.0
24	1444.0
25	1413.0
26	1382.0
27	1619.0
28	1856.0
29	2190.0
30	2524.0
31	3185.5
32	3847.0
33	3996.0
34	4145.0
35	4878.5
36	5612.0
37	6199.5
38	6787.0
39	8233.0
40	9679.0
41	12330.0
42	14981.0
43	16814.5
44	18648.0
45	18557.0
46	18466.0
47	18178.5
48	17891.0
49	17419.5
50	16948.0
51	16023.5
52	15099.0
53	14129.0
54	13159.0
55	11717.5
56	10276.0
57	9818.0
58	9360.0
59	8659.0
60	7958.0
61	7350.5
62	6743.0
63	6085.5
64	5428.0
65	4691.5
66	3955.0
67	3311.5
68	2668.0
69	2259.5
70	1851.0
71	1559.0
72	1267.0
73	1039.5
74	812.0
75	650.5
76	489.0
77	364.0
78	239.0
79	178.5
80	118.0
81	88.0
82	58.0
83	50.0
84	42.0
85	30.5
86	19.0
87	13.5
88	8.0
89	6.5
90	5.0
91	5.5
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	229086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.88826030399064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.26950361828378	30.566250229171576
2	10.189209270134656	8.739949189387392
3	4.9729773742760885	6.398470443414264
4	3.044243824490336	5.222492862942301
5	2.0030330480096894	4.295330138026767
6	1.4839543617876663	3.818653256855504
7	1.1297594935420505	3.391739346795527
8	0.8987185881059736	3.083558139737915
9	0.7226389553286989	2.7893454859746996
>10	4.26255203509379	29.354478230882723
>50	0.019338225565134197	0.5155269200213021
>100	0.003053404036600136	0.16805915682320177
>500	0.0	0.0
>1k	0.0010178013455333789	1.6561465999668248
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3794	1.6561465999668248	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01658765703709524	0.0
2	0.0	0.0	0.0	0.08250176789502632	0.0
3	0.0	0.0	0.0	0.11829618571191605	0.0
4	0.0	0.0	0.0	0.18202771011759775	0.0
5	0.0	0.0	0.0	0.36667452397789474	0.0
6	0.0	0.0	0.0	0.5329876116392971	0.0
7	0.0	0.0	0.0	0.6456090725753647	0.0
8	0.0	0.0	0.0	0.909702033297539	0.0
9	0.0	0.0	0.0	1.0249425979763058	0.0
10	0.0	0.0	0.0	1.2663366595950865	0.0
11	0.0	0.0	0.0	1.4278480570615402	0.0
12	0.0	0.0	0.0	1.630392079830282	0.0
13	0.0	0.0	0.0	1.7011078808831617	0.0
14	4.3651729044987474E-4	0.0	0.0	1.7246798145674551	0.0
15	4.3651729044987474E-4	0.0	0.0	1.7661489571601932	0.0
16	8.730345808997495E-4	0.0	0.0	1.8582541054451167	0.0
17	8.730345808997495E-4	0.0	0.0	1.947303632696891	0.0
18	8.730345808997495E-4	0.0	0.0	2.09048130396445	0.0
19	8.730345808997495E-4	0.0	0.0	2.158141483984181	0.0
20	8.730345808997495E-4	0.0	0.0	2.2393337000078573	0.0
21	8.730345808997495E-4	0.0	0.0	2.3262006408073823	0.0
22	8.730345808997495E-4	0.0	0.0	2.420488375544555	0.0
23	8.730345808997495E-4	0.0	0.0	2.5169586967339774	0.0
24	8.730345808997495E-4	0.0	0.0	2.5937857398531556	0.0
25	8.730345808997495E-4	0.0	0.0	2.656207712387488	0.0
26	8.730345808997495E-4	0.0	0.0	2.7129549601459715	0.0
27	8.730345808997495E-4	0.0	0.0	2.7814881747466016	0.0
28	8.730345808997495E-4	0.0	0.0	2.8417275608286845	0.0
29	8.730345808997495E-4	0.0	0.0	2.9159355002051632	0.0
30	8.730345808997495E-4	0.0	0.0	3.003238958295138	0.0
31	8.730345808997495E-4	0.0	0.0	3.077883414962067	0.0
32	8.730345808997495E-4	0.0	0.0	3.156893044533494	0.0
33	8.730345808997495E-4	0.0	0.0	3.234156604943122	0.0
34	8.730345808997495E-4	0.0	0.0	3.3227696149044466	0.0
35	8.730345808997495E-4	0.0	0.0	3.4397562487450126	0.0
36	8.730345808997495E-4	0.0	0.0	3.5279327414158876	0.0
37	8.730345808997495E-4	0.0	0.0	3.6108710266013637	0.0
38	8.730345808997495E-4	0.0	0.0	3.703412692176737	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	30	2.5188128E-6	44.000004	2
CGGGTAG	20	7.8432716E-4	44.0	6
CGAACGG	20	7.8432716E-4	44.0	2
GACCGAT	45	4.765752E-10	44.0	9
ACGGGAC	35	1.4392936E-7	44.0	5
ATCGTAG	20	7.8432716E-4	44.0	1
GTATGAT	20	7.8432716E-4	44.0	9
TCGTAAG	25	4.4316494E-5	44.0	1
CGTAAGG	50	2.7284841E-11	44.0	2
TATACGG	20	7.8432716E-4	44.0	2
CGTTTTT	2420	0.0	43.18182	1
GGGCGAT	255	0.0	41.411766	7
TGGGCGA	85	0.0	41.411766	6
AACGGGA	55	7.6397555E-11	40.0	4
CTTTAGG	50	1.3369572E-9	39.6	2
AGGGCGA	145	0.0	39.448273	6
GAACGGG	95	0.0	39.368423	3
GCGCGAC	45	2.3368557E-8	39.11111	9
CGACCCG	45	2.3368557E-8	39.11111	32
CGCGACC	45	2.3368557E-8	39.11111	10
>>END_MODULE