FastQCFastQC Report
Thu 26 May 2016
SRR1546019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546019_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences225137
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37001.6434437697935036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA207.84298E-444.00000413
GCCTTAC207.84298E-444.00000426
ACTGCCA207.84298E-444.00000423
TGTTCGA207.84298E-444.00000440
GTAAGGC207.84298E-444.0000049
ATTGCGG207.84298E-444.0000042
TTTAGCG254.4314038E-544.01
CGGGATT254.4314038E-544.06
CTCGAGG254.4314038E-544.02
CGATAGT254.4314038E-544.010
AAACGGG351.439148E-743.9999963
TTGGGCA700.043.9999965
CGTTTTT24100.042.995851
AGGGATC850.041.4117666
CTAGGGA750.041.066674
AGGGCGA2000.040.76
TAGGGTA1150.040.1739165
CGGGCGA557.6397555E-1140.06
AAGGGCG557.6397555E-1140.05
ACGGGCG501.3369572E-939.6000025
Produced by FastQC (version 0.11.5)