FastQCFastQC Report
Thu 26 May 2016
SRR1546018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546018_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences192411
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19271.001501993129291No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT3660.1902178149897875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT650.044.0000048
CGGGTCT207.840129E-444.06
ATAGGCG551.8189894E-1244.04
CGAAAGG302.516701E-644.02
CTATAGG502.7284841E-1144.02
ACCGATA302.516701E-644.010
TCCTCAA302.516701E-644.021
AGGGTAC207.840129E-444.06
CGTTAGG254.4289907E-544.02
ATTCCCC207.840129E-444.011
ACGATGG207.840129E-444.02
CGTAGGC254.4289907E-544.03
TTTGGCG408.250936E-944.04
GACGGCG207.840129E-444.04
CATACGA408.250936E-944.018
TCCGTCT207.840129E-444.030
CGTAAGG254.4289907E-544.02
TTACGAG207.840129E-444.01
ATACGAA408.250936E-944.019
GTTGGCG351.4376928E-744.04
Produced by FastQC (version 0.11.5)