FastQCFastQC Report
Thu 26 May 2016
SRR1546017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546017_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences686734
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102981.4995616934650098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.8542664E-444.037
GGCGTAA207.8542664E-444.039
TAACGTG207.8542664E-444.043
TTACGAG207.8542664E-444.01
CGGTCTA551.8189894E-1244.031
GCGTAAT207.8542664E-444.040
CGTTTTT62800.043.0191081
CGGGAAC557.8216544E-1140.06
TACGGGA2250.039.111114
TTAACGG452.3484972E-839.111112
TATACGG850.038.8235322
ACGGGAG2500.038.725
ACCCGCT2400.038.534
ATAGGGC3150.038.41274
GTTTTTT73000.038.3945242
TAACGGG1900.038.2105263
ATAGGGA5300.038.1886834
CGCGACC2500.037.8410
TACGGGT357.2859275E-637.7142874
TTATACG357.2859275E-637.7142871
Produced by FastQC (version 0.11.5)