##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546017_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	686734
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.001611977854598	31.0	28.0	33.0	23.0	33.0
2	28.709241132665632	31.0	28.0	33.0	16.0	33.0
3	28.213238313524595	31.0	27.0	33.0	16.0	34.0
4	32.332329548267595	35.0	32.0	35.0	26.0	37.0
5	33.67937658540279	35.0	33.0	35.0	28.0	37.0
6	33.4230706503537	35.0	33.0	37.0	28.0	37.0
7	34.49634356242738	35.0	35.0	37.0	32.0	37.0
8	34.83954049166053	35.0	35.0	37.0	32.0	37.0
9	36.561923539536416	39.0	37.0	39.0	32.0	39.0
10	36.10749868216806	38.0	35.0	39.0	31.0	39.0
11	36.27584625196947	38.0	35.0	39.0	31.0	39.0
12	35.09218998913699	37.0	34.0	39.0	29.0	39.0
13	35.63535226157435	37.0	35.0	39.0	30.0	39.0
14	36.2735964725789	38.0	35.0	40.0	30.0	41.0
15	36.75437068792284	38.0	35.0	40.0	31.0	41.0
16	36.312694580434346	38.0	34.0	40.0	31.0	41.0
17	35.93345458357967	38.0	34.0	40.0	30.0	41.0
18	35.54721770001194	37.0	34.0	39.0	29.0	41.0
19	35.05332049964033	37.0	33.0	39.0	27.0	41.0
20	35.606901653333026	37.0	34.0	39.0	30.0	41.0
21	35.84995937291586	37.0	34.0	40.0	30.0	41.0
22	36.21324705053194	38.0	35.0	40.0	31.0	41.0
23	36.53848506117361	38.0	35.0	40.0	32.0	41.0
24	36.21619433434197	37.0	35.0	40.0	31.0	41.0
25	35.86836533504967	37.0	35.0	40.0	30.0	41.0
26	35.99794389093885	37.0	35.0	40.0	31.0	41.0
27	36.28354355543777	38.0	35.0	40.0	31.0	41.0
28	36.20761022462846	38.0	35.0	40.0	31.0	41.0
29	36.36684072726849	38.0	35.0	40.0	31.0	41.0
30	35.995656251183135	38.0	35.0	40.0	31.0	41.0
31	35.34642670961391	37.0	34.0	40.0	29.0	41.0
32	35.39387302798463	37.0	34.0	40.0	29.0	41.0
33	35.168509204437235	38.0	34.0	40.0	27.0	41.0
34	34.92924043370505	38.0	34.0	40.0	25.0	41.0
35	34.712759234288676	38.0	34.0	40.0	24.0	41.0
36	34.33543701054557	37.0	34.0	40.0	23.0	41.0
37	34.26383432304211	37.0	34.0	40.0	22.0	41.0
38	34.105861949459324	37.0	33.0	40.0	21.0	41.0
39	33.88878954587948	37.0	33.0	40.0	21.0	41.0
40	33.705182792755274	36.0	33.0	40.0	20.0	41.0
41	33.41514327235873	36.0	33.0	40.0	18.0	40.0
42	33.58040231006474	36.0	33.0	40.0	18.0	41.0
43	33.36464628225776	36.0	33.0	40.0	18.0	40.0
44	33.23915810197253	36.0	33.0	40.0	18.0	40.0
45	33.164961979456386	36.0	33.0	39.0	18.0	40.0
46	32.967667248163046	35.0	32.0	39.0	18.0	40.0
47	32.941796969423386	35.0	32.0	39.0	18.0	40.0
48	32.92761826267521	35.0	32.0	39.0	18.0	40.0
49	32.89936569326697	35.0	32.0	39.0	18.0	40.0
50	33.10283894491899	35.0	33.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	2.0
13	10.0
14	33.0
15	69.0
16	154.0
17	334.0
18	678.0
19	1144.0
20	1771.0
21	2593.0
22	3614.0
23	5256.0
24	7341.0
25	11046.0
26	15695.0
27	18200.0
28	18208.0
29	18104.0
30	20447.0
31	24850.0
32	33203.0
33	48874.0
34	74658.0
35	65192.0
36	73639.0
37	103600.0
38	114222.0
39	23794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.77232523801064	23.39188099031066	28.195924477308537	20.639869294370165
2	35.39885894684113	20.926443135187714	30.736500595572668	12.938197322398484
3	28.496040679506184	19.16913972513375	38.64596772549488	13.688851869865188
4	25.416391208240746	25.813488191934574	34.56695023109385	14.203170368730833
5	20.894698675178454	29.766255930243734	35.49642219549345	13.842623199084361
6	18.540512046876955	38.529911144635335	32.577824892898846	10.35175191558886
7	79.15437418272577	4.71041189164946	13.617790876816935	2.5174230488078355
8	80.54268464936933	3.2757952860933055	13.35262270398728	2.828897360550082
9	76.90998261335538	4.832438760859372	14.472852662020522	3.7847259637647177
10	41.20940567963724	23.48391080097971	21.551867244085773	13.754816275297276
11	34.874201655954124	22.947604166970034	27.486042630771156	14.692151546304682
12	31.844498743327108	21.181272515996007	31.29392166399217	15.680307076684713
13	21.820384603063196	29.140540587767603	31.799357538726785	17.239717270442412
14	17.316602935052	31.29348481362507	33.948661344858415	17.44125090646451
15	17.316020467895868	22.641663293211053	42.64795393849729	17.394362300395784
16	19.314174047010923	19.94207364132255	41.46423506044553	19.279517251220994
17	19.29116659434366	20.513182687911186	33.666747241289926	26.528903476455223
18	21.839314785637526	22.15908925435467	36.21620598368509	19.78538997632271
19	25.28271499590817	23.60986932349352	31.202911170846352	19.904504509751956
20	26.42405938835124	22.75451630471187	31.454682599084943	19.366741707851947
21	22.13666426884354	25.030652334091513	33.391677126806016	19.44100627025894
22	22.52837343134314	22.254905101538586	32.113307335882595	23.103414131235674
23	20.66651716676326	25.55429030745529	31.72087008943783	22.058322436343623
24	19.791214647884043	23.095405207838844	38.227610690602184	18.885769453674932
25	19.439695719157633	23.587589954771428	36.14951349430784	20.8232008317631
26	18.820533132188007	27.16219089196108	32.66053522906977	21.356740746781142
27	18.382809064353882	27.54953155078968	33.60922860962178	20.458430775234664
28	17.425961143616014	25.651999172896637	37.83196987479868	19.09006980868866
29	18.684818284808966	23.501384815663705	36.80406096101257	21.00973593851477
30	20.367420282088844	25.859648714058135	34.467202730606026	19.30572827324699
31	25.054533487492975	24.507888061461934	30.674759077022546	19.76281937402255
32	25.403576930805816	23.961097018641862	31.195484714605655	19.43984133594667
33	24.486336776685004	25.298004758756665	30.13801559264577	20.07764287191256
34	20.404698180081372	25.322468379314262	32.06496256192354	22.207870878680826
35	20.865138467004694	25.11729432356633	32.293872154283896	21.72369505514508
36	25.364697248133922	25.73267087402109	28.67762481543072	20.225007062414267
37	20.839073061767728	28.643987337164024	30.047296333078016	20.469643267990225
38	21.437121214327526	29.341200523055505	27.817320825821934	21.40435743679503
39	21.284660436209656	28.005312100463936	28.475799945830556	22.234227517495857
40	22.79164858591536	25.632486523166175	28.92590144073251	22.649963450185954
41	19.654917333348866	23.955126730291497	30.14500519851937	26.244950737840274
42	21.91707415098131	24.951728034435458	27.881100979418523	25.250096835164708
43	21.11195892441615	25.481045062571532	29.376731019579633	24.03026499343268
44	20.707435484481618	26.934591850702017	29.89556363890531	22.46240902591105
45	19.81975553853457	30.32061904609354	27.506720214813885	22.352905200558006
46	21.818491584805763	28.330474390375315	28.785381239315367	21.06565278550356
47	21.137150628918913	26.774267765976344	29.734074619867375	22.35450698523737
48	21.50672603948545	25.04492277941678	31.474049632026375	21.974301549071402
49	21.01920102980193	25.003130760964215	31.270768594535873	22.706899614697974
50	20.048082663738796	27.885032632722424	30.738102380252037	21.328782323286745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	242.0
1	533.5
2	825.0
3	8510.5
4	16196.0
5	11430.0
6	6664.0
7	6614.0
8	6564.0
9	6767.5
10	6971.0
11	6872.5
12	6774.0
13	6403.5
14	6033.0
15	5709.5
16	5386.0
17	5034.5
18	4683.0
19	4275.0
20	3867.0
21	3845.0
22	3823.0
23	4068.0
24	4313.0
25	4337.5
26	4362.0
27	5085.5
28	5809.0
29	7097.0
30	8385.0
31	10154.0
32	11923.0
33	13410.5
34	14898.0
35	16920.5
36	18943.0
37	20182.0
38	21421.0
39	25409.0
40	29397.0
41	36993.0
42	44589.0
43	48886.0
44	53183.0
45	54855.0
46	56527.0
47	56076.0
48	55625.0
49	54245.0
50	52865.0
51	49516.5
52	46168.0
53	42244.5
54	38321.0
55	35035.0
56	31749.0
57	29994.0
58	28239.0
59	26362.0
60	24485.0
61	22513.0
62	20541.0
63	18079.0
64	15617.0
65	13039.0
66	10461.0
67	8846.5
68	7232.0
69	6276.0
70	5320.0
71	4401.5
72	3483.0
73	2767.5
74	2052.0
75	1712.5
76	1373.0
77	1050.0
78	727.0
79	528.0
80	329.0
81	259.0
82	189.0
83	136.0
84	83.0
85	63.0
86	43.0
87	37.5
88	32.0
89	21.0
90	10.0
91	8.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	686734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.33253405650448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.39173322762551	24.129999132192147
2	8.372303149319938	5.581401597121731
3	4.150054706598541	4.149955195321579
4	2.667321040289094	3.5563427766026883
5	1.9473350268154468	3.245480555037498
6	1.3879392897635663	2.7758120186642827
7	1.0911349256956147	2.5459204449693393
8	0.8893078666576697	2.37143078016673
9	0.7255028733052661	2.1764564309285674
>10	6.05867461256843	40.74464087012996
>50	0.299431599537964	6.216266910672387
>100	0.01838615083102219	0.9151997847896591
>500	4.3776549597671874E-4	0.08842650981864372
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.3776549597671874E-4	1.502666993584807
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10298	1.4995616934650098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037569131570593564	0.0
2	0.0	0.0	0.0	0.1338218291216104	0.0
3	1.4561678903330839E-4	0.0	0.0	0.18537017243940157	0.0
4	1.4561678903330839E-4	0.0	0.0	0.262547070627055	0.0
5	1.4561678903330839E-4	0.0	0.0	0.44733477591032333	0.0
6	1.4561678903330839E-4	0.0	0.0	0.6465385433078892	0.0
7	1.4561678903330839E-4	0.0	0.0	0.7876412118811651	0.0
8	1.4561678903330839E-4	0.0	0.0	1.149353315839903	0.0
9	1.4561678903330839E-4	0.0	0.0	1.3321023860767052	0.0
10	1.4561678903330839E-4	0.0	0.0	1.6326554386414536	0.0
11	1.4561678903330839E-4	0.0	0.0	1.8596720127443813	0.0
12	1.4561678903330839E-4	0.0	0.0	2.0676127874839456	0.0
13	1.4561678903330839E-4	0.0	0.0	2.1581864302626634	0.0
14	1.4561678903330839E-4	0.0	0.0	2.1977941968797237	0.0
15	1.4561678903330839E-4	0.0	0.0	2.242061700745849	0.0
16	1.4561678903330839E-4	0.0	0.0	2.3358389128833	0.0
17	1.4561678903330839E-4	0.0	0.0	2.455099063101579	0.0
18	1.4561678903330839E-4	0.0	0.0	2.6336252464564156	0.0
19	1.4561678903330839E-4	0.0	0.0	2.7140057140028016	0.0
20	2.9123357806661677E-4	0.0	0.0	2.8061811414608857	0.0
21	2.9123357806661677E-4	0.0	0.0	2.908112893784202	0.0
22	2.9123357806661677E-4	0.0	0.0	3.002763806655852	0.0
23	2.9123357806661677E-4	0.0	0.0	3.121295872928965	0.0
24	2.9123357806661677E-4	0.0	0.0	3.2163836361677154	0.0
25	2.9123357806661677E-4	0.0	0.0	3.292686833621169	0.0
26	2.9123357806661677E-4	0.0	0.0	3.3637478266694236	0.0
27	2.9123357806661677E-4	0.0	0.0	3.4547583198152414	0.0
28	2.9123357806661677E-4	0.0	0.0	3.5379055063532605	0.0
29	2.9123357806661677E-4	0.0	0.0	3.6348862878494437	0.0
30	2.9123357806661677E-4	0.0	0.0	3.7502147847638243	0.0
31	2.9123357806661677E-4	0.0	0.0	3.849816668462607	0.0
32	2.9123357806661677E-4	0.0	0.0	3.939516610507125	0.0
33	5.824671561332335E-4	0.0	0.0	4.033876289800709	0.0
34	5.824671561332335E-4	0.0	0.0	4.138720377904691	0.0
35	5.824671561332335E-4	0.0	0.0	4.259145462435237	0.0
36	5.824671561332335E-4	0.0	0.0	4.360203514024353	0.0
37	5.824671561332335E-4	0.0	0.0	4.465921302862535	0.0
38	7.28083945166542E-4	0.0	0.0	4.580376099042716	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.8542664E-4	44.0	37
GGCGTAA	20	7.8542664E-4	44.0	39
TAACGTG	20	7.8542664E-4	44.0	43
TTACGAG	20	7.8542664E-4	44.0	1
CGGTCTA	55	1.8189894E-12	44.0	31
GCGTAAT	20	7.8542664E-4	44.0	40
CGTTTTT	6280	0.0	43.019108	1
CGGGAAC	55	7.8216544E-11	40.0	6
TACGGGA	225	0.0	39.11111	4
TTAACGG	45	2.3484972E-8	39.11111	2
TATACGG	85	0.0	38.823532	2
ACGGGAG	250	0.0	38.72	5
ACCCGCT	240	0.0	38.5	34
ATAGGGC	315	0.0	38.4127	4
GTTTTTT	7300	0.0	38.394524	2
TAACGGG	190	0.0	38.210526	3
ATAGGGA	530	0.0	38.188683	4
CGCGACC	250	0.0	37.84	10
TACGGGT	35	7.2859275E-6	37.714287	4
TTATACG	35	7.2859275E-6	37.714287	1
>>END_MODULE