FastQCFastQC Report
Thu 26 May 2016
SRR1546016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546016_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences691886
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107581.554880428278647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATT207.8543066E-444.024
CGAAACT302.5262489E-644.044
TCGATAG502.7284841E-1144.01
CGTTTTT64350.043.0427361
TCGATTG452.3486791E-839.111111
TAGTGCG452.3486791E-839.111111
CGATAGA452.3486791E-839.1111110
CAATTAT4850.039.01030744
GGCGATA2150.038.883728
GTTTTTT74250.038.7555542
GCGCGAC2000.038.59
ATAGGGA6800.038.176474
GGGCGAT9150.037.7486347
ATAGGGC4200.037.7142874
TACGGGA1400.037.7142874
ACGGGTA700.037.7142875
AATACGG357.2860057E-637.7142872
AGGGCGA5250.037.7142836
TAGGGCA5500.037.65
GTATAGG3000.037.42
Produced by FastQC (version 0.11.5)