##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546016_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	691886
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.970149706743598	31.0	28.0	33.0	23.0	33.0
2	28.672161309811155	31.0	28.0	33.0	16.0	33.0
3	28.211479925883744	31.0	27.0	33.0	16.0	34.0
4	32.32623437965214	35.0	32.0	35.0	26.0	37.0
5	33.70805161543954	35.0	33.0	35.0	28.0	37.0
6	33.50882948925112	35.0	33.0	37.0	28.0	37.0
7	34.555611762631415	35.0	35.0	37.0	32.0	37.0
8	34.86442997834904	35.0	35.0	37.0	32.0	37.0
9	36.657751421476945	39.0	37.0	39.0	32.0	39.0
10	36.090545263237004	38.0	35.0	39.0	31.0	39.0
11	36.32285954622582	38.0	35.0	39.0	32.0	39.0
12	35.03777934515224	37.0	34.0	39.0	28.0	39.0
13	35.59620515518453	37.0	35.0	39.0	30.0	39.0
14	36.2678909531339	38.0	35.0	40.0	30.0	41.0
15	36.78296713620452	38.0	35.0	40.0	31.0	41.0
16	36.32725477896648	38.0	34.0	40.0	31.0	41.0
17	35.96823031539878	38.0	34.0	40.0	30.0	41.0
18	35.618958903634415	38.0	34.0	39.0	29.0	41.0
19	35.11472410194743	37.0	33.0	39.0	27.0	41.0
20	35.562007324906126	37.0	34.0	39.0	30.0	41.0
21	35.71890745007126	37.0	34.0	39.0	30.0	40.0
22	36.16079961149669	38.0	35.0	40.0	31.0	41.0
23	36.542196257765006	38.0	35.0	40.0	32.0	41.0
24	36.059694805213574	37.0	35.0	40.0	31.0	41.0
25	35.74686003185496	37.0	34.0	40.0	30.0	41.0
26	35.948643273602876	37.0	35.0	40.0	31.0	41.0
27	36.28586790309386	38.0	35.0	40.0	31.0	41.0
28	36.19720878873109	38.0	35.0	40.0	31.0	41.0
29	36.35827578531723	38.0	35.0	40.0	31.0	41.0
30	36.015118097490046	38.0	35.0	40.0	31.0	41.0
31	35.345938492757476	37.0	34.0	40.0	29.0	41.0
32	35.43083542664543	37.0	34.0	40.0	29.0	41.0
33	35.22933402323504	38.0	34.0	40.0	28.0	41.0
34	34.92477807037576	38.0	34.0	40.0	25.0	41.0
35	34.73809269157058	38.0	34.0	40.0	24.0	41.0
36	34.343412064993366	37.0	34.0	40.0	23.0	41.0
37	34.26497428767167	37.0	34.0	40.0	22.0	41.0
38	34.1506678845937	37.0	34.0	40.0	22.0	41.0
39	33.87718641510307	37.0	33.0	40.0	21.0	41.0
40	33.67761596563596	36.0	33.0	40.0	20.0	41.0
41	33.38070722633498	36.0	33.0	40.0	18.0	40.0
42	33.5581699875413	36.0	33.0	40.0	18.0	40.0
43	33.341407399484886	36.0	33.0	40.0	18.0	40.0
44	33.21633188126368	36.0	33.0	40.0	18.0	40.0
45	33.14601104806283	36.0	33.0	39.0	18.0	40.0
46	32.93112593693181	35.0	32.0	39.0	18.0	40.0
47	32.89841390055587	35.0	32.0	39.0	18.0	40.0
48	32.899571605726955	35.0	32.0	39.0	18.0	40.0
49	32.92518709729637	35.0	32.0	39.0	18.0	40.0
50	33.158138768525454	35.0	33.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	5.0
13	8.0
14	26.0
15	53.0
16	159.0
17	356.0
18	634.0
19	1138.0
20	1674.0
21	2599.0
22	3699.0
23	5057.0
24	7404.0
25	11091.0
26	15732.0
27	18570.0
28	18278.0
29	18421.0
30	20447.0
31	24957.0
32	33275.0
33	49267.0
34	75880.0
35	65502.0
36	75181.0
37	106465.0
38	114962.0
39	21039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.795185912130034	23.4437465131539	27.986113319246236	20.774954255469833
2	35.61583844737427	20.822216376686335	30.669214292527958	12.89273088341143
3	28.419710761599454	19.069760047175404	38.82778376784615	13.682745423378995
4	25.218605377186414	25.88648997089116	34.579106962707726	14.315797689214696
5	20.90676787794521	29.825433669708595	35.397594401389824	13.870204050956373
6	18.684580985884956	38.34735780171878	32.72533336416693	10.242727848229332
7	78.89001945407192	4.819146506794472	13.718444946132744	2.57238909300087
8	80.27348436014026	3.3311846171190056	13.52809566893968	2.8672353538010595
9	76.69775078553404	4.893291669436872	14.66253111061649	3.746426434412606
10	41.11472121129782	23.212205478937282	21.63521158109862	14.037861728666284
11	34.842589675177706	22.878046383363735	27.572461359241263	14.706902582217301
12	31.964658917798598	20.774954255469833	31.29966497370954	15.960721853022031
13	21.588383057324474	29.162896777792874	31.91884212138994	17.329878043492712
14	17.404601336058253	31.27148113995658	33.89214408153944	17.43177344244572
15	17.36557756624646	22.700705029441266	42.77062984364476	17.16308756066751
16	19.2180503724602	19.880009134452788	41.614225464888726	19.28771502819829
17	19.178448472725275	20.586917497969317	33.69196659565304	26.54266743365237
18	21.819056896656384	22.20756020500487	36.21391385286015	19.75946904547859
19	25.11338573117537	23.653752207733643	31.261797463744028	19.971064597346963
20	26.36951752167265	22.377530402407334	31.60925354754974	19.643698528370283
21	22.07112154314439	25.033314736820806	33.41518689495091	19.4803768250839
22	22.640579517434954	22.02443755185103	32.139832284509296	23.19515064620472
23	20.704856002289397	25.48440060934894	31.75971186004631	22.05103152831536
24	20.01370167917836	22.857378238611563	38.273068106595595	18.855851975614478
25	19.24160916682806	23.6569319223109	36.100600387925184	21.000858522935857
26	18.74398383548735	27.043906077012686	32.68833304908612	21.523777038413844
27	18.37571507444868	27.4812613638663	33.60322365245141	20.5397999092336
28	17.35069072072567	25.693828174005546	37.87184593993808	19.083635165330705
29	18.63934231939944	23.427414342825262	36.85549353506213	21.077749802713164
30	20.227031620816145	25.832579355558575	34.52693073714455	19.41345828648072
31	24.983595563430967	24.45055977429808	30.792645031117843	19.77319963115311
32	25.251414250324476	23.967387691035807	31.218582251989492	19.56261580665023
33	24.48958354410987	25.285523915789593	30.215093237903357	20.00979930219718
34	20.36390388011898	25.45072454132617	32.056870640539046	22.1285009380158
35	20.818313999705154	25.19403485545307	32.28017332335096	21.707477821490823
36	25.38568492497319	25.765660816955393	28.679580162049817	20.1690740960216
37	20.752551720948247	28.55007905926699	30.18980005376608	20.50756916601868
38	21.52392157089463	29.072853042264192	27.976718707995246	21.426506678845936
39	21.21115328247717	27.856034086540266	28.635642287891354	22.297170343091203
40	22.77557285448759	25.63818316890355	29.01576271235435	22.570481264254518
41	19.61869440919458	23.846269472138474	30.38636422763288	26.14867189103407
42	22.02024610990828	24.90814960846151	27.903440740237556	25.16816354139266
43	21.259282598578377	25.485556869195214	29.35136713273574	23.903793399490667
44	20.84707596338125	26.832310525144315	29.815027331092114	22.505586180382316
45	19.877696614760236	30.324793390818716	27.454811919882754	22.34269807453829
46	21.913581139089388	28.295123763163293	28.757049571750258	21.034245525997058
47	21.06618720425041	26.816845549700385	29.798984225725043	22.31798302032416
48	21.58274629057388	25.065979077478083	31.473103950650827	21.878170681297206
49	21.086710816521798	24.972177497449003	31.212222822834974	22.728888863194225
50	20.105335271995674	27.85964739855988	30.69681999635778	21.338197333086665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	518.0
2	835.0
3	8866.5
4	16898.0
5	11806.5
6	6715.0
7	6612.0
8	6509.0
9	6762.0
10	7015.0
11	6948.5
12	6882.0
13	6520.5
14	6159.0
15	5779.0
16	5399.0
17	5079.5
18	4760.0
19	4353.0
20	3946.0
21	3837.0
22	3728.0
23	4040.5
24	4353.0
25	4326.5
26	4300.0
27	4986.0
28	5672.0
29	7000.5
30	8329.0
31	10209.5
32	12090.0
33	13427.0
34	14764.0
35	16952.5
36	19141.0
37	20294.0
38	21447.0
39	25462.5
40	29478.0
41	37110.0
42	44742.0
43	49094.5
44	53447.0
45	55271.0
46	57095.0
47	56559.0
48	56023.0
49	54608.0
50	53193.0
51	49939.0
52	46685.0
53	42683.0
54	38681.0
55	35492.0
56	32303.0
57	29962.0
58	27621.0
59	26134.0
60	24647.0
61	22729.5
62	20812.0
63	18414.5
64	16017.0
65	13351.0
66	10685.0
67	9011.0
68	7337.0
69	6421.0
70	5505.0
71	4533.0
72	3561.0
73	2803.5
74	2046.0
75	1730.5
76	1415.0
77	1063.5
78	712.0
79	544.0
80	376.0
81	286.0
82	196.0
83	133.5
84	71.0
85	54.5
86	38.0
87	34.0
88	30.0
89	23.5
90	17.0
91	11.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	691886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.146237134875165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08495584612785	23.893450403327595
2	8.434202323681346	5.591241405285141
3	4.28911701508573	4.2650426904377845
4	2.709007811592695	3.5917366129312276
5	1.8976894300193077	3.1450631927883026
6	1.4244830310725587	2.832975140552206
7	1.0996069064972636	2.5513481894533356
8	0.9039628467178027	2.3970373502740077
9	0.7394366912162619	2.2058589521953507
>10	6.09547278229465	40.713263738688326
>50	0.3031719888705887	6.252377906346098
>100	0.018014567436771337	0.9063042481785547
>500	4.393796935797887E-4	0.08752833892902322
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.393796935797887E-4	1.5667718306130314
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10758	1.554880428278647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03772297748473014	0.0
2	0.0	0.0	0.0	0.1279112454941999	0.0
3	0.0	0.0	0.0	0.17618509407619173	0.0
4	0.0	0.0	0.0	0.25192011400722086	0.0
5	0.0	0.0	0.0	0.43836701421910546	0.0
6	0.0	0.0	0.0	0.6398452924325684	0.0
7	0.0	0.0	0.0	0.785100435620897	0.0
8	0.0	0.0	0.0	1.1562598462752534	0.0
9	0.0	0.0	0.0	1.3396715643906656	0.0
10	0.0	0.0	0.0	1.6421780466724287	0.0
11	0.0	0.0	0.0	1.8569533131180571	0.0
12	0.0	0.0	0.0	2.0581425263699513	0.0
13	0.0	0.0	0.0	2.1467409370907924	0.0
14	0.0	0.0	0.0	2.18012794015199	0.0
15	0.0	0.0	0.0	2.2292689836186885	0.0
16	0.0	0.0	0.0	2.327551070552085	0.0
17	0.0	0.0	0.0	2.4342160413709775	0.0
18	0.0	0.0	0.0	2.6072214208699123	0.0
19	0.0	0.0	0.0	2.682956440800941	0.0
20	0.0	0.0	0.0	2.7753126960221772	0.0
21	0.0	0.0	0.0	2.8832784591681286	0.0
22	0.0	0.0	0.0	2.988787170140746	0.0
23	1.4453248078440668E-4	0.0	0.0	3.1023897000372895	0.0
24	1.4453248078440668E-4	0.0	0.0	3.188531058584796	0.0
25	1.4453248078440668E-4	0.0	0.0	3.2600746365730773	0.0
26	1.4453248078440668E-4	0.0	0.0	3.331907279522927	0.0
27	1.4453248078440668E-4	0.0	0.0	3.415013455973961	0.0
28	1.4453248078440668E-4	0.0	0.0	3.4943617879246003	0.0
29	1.4453248078440668E-4	0.0	0.0	3.583393796087795	0.0
30	2.8906496156881335E-4	0.0	0.0	3.7001760405615953	0.0
31	4.3359744235322006E-4	0.0	0.0	3.7922432308212626	0.0
32	4.3359744235322006E-4	0.0	0.0	3.8902362527930903	0.0
33	4.3359744235322006E-4	0.0	0.0	3.9846159627453077	0.0
34	4.3359744235322006E-4	0.0	0.0	4.0765386205241905	0.0
35	5.781299231376267E-4	0.0	0.0	4.192742735074853	0.0
36	5.781299231376267E-4	0.0	0.0	4.295216263950998	0.0
37	5.781299231376267E-4	0.0	0.0	4.394510078249885	0.0
38	5.781299231376267E-4	0.0	0.0	4.517796284358984	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATT	20	7.8543066E-4	44.0	24
CGAAACT	30	2.5262489E-6	44.0	44
TCGATAG	50	2.7284841E-11	44.0	1
CGTTTTT	6435	0.0	43.042736	1
TCGATTG	45	2.3486791E-8	39.11111	1
TAGTGCG	45	2.3486791E-8	39.11111	1
CGATAGA	45	2.3486791E-8	39.11111	10
CAATTAT	485	0.0	39.010307	44
GGCGATA	215	0.0	38.88372	8
GTTTTTT	7425	0.0	38.755554	2
GCGCGAC	200	0.0	38.5	9
ATAGGGA	680	0.0	38.17647	4
GGGCGAT	915	0.0	37.748634	7
ATAGGGC	420	0.0	37.714287	4
TACGGGA	140	0.0	37.714287	4
ACGGGTA	70	0.0	37.714287	5
AATACGG	35	7.2860057E-6	37.714287	2
AGGGCGA	525	0.0	37.714283	6
TAGGGCA	550	0.0	37.6	5
GTATAGG	300	0.0	37.4	2
>>END_MODULE