FastQCFastQC Report
Thu 26 May 2016
SRR1546015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546015_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences683352
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105261.5403481660988771No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG207.8542373E-444.0000041
TAATGCG207.8542373E-444.0000041
CGGTCTA551.8189894E-1244.031
CGTTTTT62550.043.3317341
CGGGATA700.040.8571436
ACCCGCT1750.040.2285734
GTTTTTT71500.039.1384622
TACGGGT452.3484972E-839.111114
TAGGGTG3050.038.950825
CAATTAT4450.038.06741744
ACGTTAG357.2858747E-637.7142871
CGCGACC2050.037.56097410
ACACGCG1000.037.39999836
GCGCGAC2000.037.3999989
GTTGATC2300.037.30434816
GACGGTC651.0913936E-1137.2307729
TACGGGA1550.036.9032254
GGGCGAT7100.036.873247
TTGCGGG4000.036.853
CGAAAGG900.036.6666682
Produced by FastQC (version 0.11.5)