FastQCFastQC Report
Thu 26 May 2016
SRR1546014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546014_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences589261
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.9355786315401835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA207.8533526E-444.00000415
ACCGTAT207.8533526E-444.00000426
TCTATAC207.8533526E-444.00000411
GTCTACG302.5256086E-644.01
TACGGGT551.8189894E-1244.04
TCTAGCG351.4444777E-744.01
CTACGGG900.044.03
CGGTCTA750.044.031
CGTTTTT56450.043.1426051
TACGGGA800.041.2500044
CGTTTGG1200.040.3333322
CGGGATA501.3460522E-939.6000026
GCGTAAG501.3460522E-939.6000021
CGGGGTA452.3475877E-839.111116
GTTTTTT64850.038.402472
TGATTGG1500.038.133332
AACTTAA7100.038.11267544
CACGGGT357.2841685E-637.7142874
TCGATTG357.2841685E-637.7142871
AAATTCG357.2841685E-637.71428730
Produced by FastQC (version 0.11.5)