##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546014_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	589261
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.644648127060844	28.0	25.0	30.0	16.0	33.0
2	25.799513288678533	30.0	23.0	31.0	10.0	33.0
3	26.22501574005407	30.0	25.0	31.0	16.0	33.0
4	28.37130405711561	32.0	19.0	35.0	10.0	37.0
5	31.169495350956538	33.0	30.0	35.0	19.0	37.0
6	29.505000330922968	33.0	28.0	35.0	17.0	36.0
7	29.421173300116585	32.0	28.0	35.0	17.0	35.0
8	32.29139719071854	33.0	32.0	35.0	28.0	35.0
9	26.793909659726335	30.0	23.0	34.0	10.0	35.0
10	30.70958200186335	34.0	27.0	37.0	17.0	37.0
11	33.32356460040627	35.0	31.0	37.0	27.0	39.0
12	32.86244295821376	35.0	31.0	37.0	23.0	39.0
13	33.41624000230797	35.0	32.0	37.0	25.0	39.0
14	33.0332772744166	35.0	31.0	38.0	22.0	40.0
15	31.819774259623493	34.0	27.0	38.0	18.0	40.0
16	30.269115044097607	33.0	26.0	38.0	16.0	40.0
17	30.563149436327876	33.0	26.0	37.0	17.0	40.0
18	31.432020785356574	34.0	27.0	38.0	17.0	39.0
19	31.105985293443823	34.0	27.0	37.0	18.0	39.0
20	31.933834073525993	34.0	30.0	37.0	19.0	39.0
21	32.896081702335636	35.0	31.0	37.0	24.0	39.0
22	33.39714489844059	35.0	31.0	38.0	25.0	39.0
23	34.648995945769364	36.0	33.0	38.0	29.0	40.0
24	34.26772177354347	35.0	33.0	38.0	27.0	40.0
25	33.01389197656047	35.0	31.0	38.0	23.0	40.0
26	26.781349860248685	33.0	21.0	37.0	0.0	39.0
27	30.115952014472363	34.0	25.0	38.0	15.0	39.0
28	31.54229789516021	34.0	29.0	37.0	20.0	39.0
29	32.8794761574243	35.0	31.0	38.0	23.0	39.0
30	32.638348711352016	35.0	31.0	38.0	23.0	39.0
31	31.456207011833467	34.0	29.0	37.0	20.0	39.0
32	31.97948616996543	34.0	30.0	37.0	21.0	39.0
33	31.748483269722584	34.0	30.0	37.0	20.0	39.0
34	31.82519291112088	35.0	30.0	38.0	18.0	39.0
35	31.114930395868722	34.0	29.0	38.0	15.0	39.0
36	30.893702451036127	34.0	29.0	38.0	15.0	39.0
37	31.029406324192504	34.0	29.0	38.0	15.0	39.0
38	30.75687853090566	34.0	29.0	37.0	14.0	39.0
39	30.541610593607928	34.0	28.0	37.0	12.0	39.0
40	30.429329278537015	34.0	28.0	37.0	13.0	39.0
41	29.8255798364392	33.0	27.0	36.0	10.0	38.0
42	30.023604141458538	33.0	27.0	37.0	10.0	38.0
43	29.55341011877589	33.0	27.0	36.0	10.0	38.0
44	29.402030679104843	33.0	26.0	36.0	10.0	38.0
45	28.883627119391917	32.0	25.0	35.0	10.0	38.0
46	28.392452580435496	32.0	24.0	35.0	9.0	38.0
47	28.452721289886824	32.0	24.0	35.0	10.0	38.0
48	28.494118904865587	32.0	24.0	35.0	10.0	38.0
49	28.31349435988467	32.0	24.0	35.0	10.0	38.0
50	30.325485650670924	34.0	28.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	11.0
12	36.0
13	63.0
14	179.0
15	358.0
16	768.0
17	1490.0
18	2488.0
19	3827.0
20	5469.0
21	7716.0
22	10380.0
23	13679.0
24	17610.0
25	22169.0
26	26859.0
27	29991.0
28	34295.0
29	40773.0
30	49640.0
31	57907.0
32	64532.0
33	66702.0
34	60314.0
35	45603.0
36	22254.0
37	4046.0
38	98.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.90719562299219	24.492881762071477	27.707925689974395	21.891996924961944
2	35.06408874844933	18.173101562804934	32.60456741579708	14.158242272948659
3	30.003852282774524	14.665487788942421	40.29131403571592	15.03934589256713
4	26.540870683788675	25.744109995401022	33.35890208243885	14.356117238371452
5	20.15236032929381	30.13690028696961	36.13526094548935	13.575478438247229
6	18.748059009505123	36.78302144550547	33.704249899450325	10.764669645539074
7	81.33899918711742	3.4091854034120703	13.433436117442016	1.8183792920284898
8	83.37578763909372	1.975864684749203	12.81571324082198	1.832634435335106
9	70.65867247280916	3.077244209272292	12.9071837437061	13.356899574212447
10	41.990221650508005	23.46820848486494	21.47265812602565	13.068911738601402
11	35.70319434002929	22.628342958383467	27.62935269770102	14.039110003886224
12	32.55705027144169	21.00580218273397	31.61451377233518	14.822633773489166
13	21.304990488085927	29.584852892012197	32.104958583717575	17.005198036184304
14	16.90201795129832	31.272729741150357	34.45858456609211	17.366667741459217
15	15.964402870714336	21.140377523711905	42.5434569740743	20.351762631499454
16	17.404002640595593	18.078406682268128	41.3784384169324	23.139152260203883
17	18.584294565566022	19.73997939792384	34.34505253190013	27.330673504610008
18	21.46264558489362	21.894881894440662	36.916748266048494	19.725724254617223
19	25.65993676825719	23.037838920274716	31.84632955515468	19.455894756313416
20	26.749437006691434	22.22275019049284	31.98463838604625	19.043174416769478
21	21.92831359957642	24.5276710999031	34.01718423584795	19.526831064672532
22	22.30318992772303	21.916604017574556	32.30945200853272	23.470754046169695
23	20.195974279648578	25.43456974074307	32.29689390609594	22.072562073512415
24	19.226963942972638	22.64107076490723	39.74927918189054	18.382686110229592
25	18.939994331883494	22.4662755553142	37.44927969100279	21.144450421799508
26	15.099760547533267	39.14309618318538	27.215444429548196	18.541698839733158
27	17.5584333597506	28.113518457865023	34.18620950648354	20.141838675900832
28	16.87452588920699	25.64483310451566	38.818621968872876	18.66201903740448
29	17.963517015380283	23.27372760118182	37.74863770044174	21.014117682996158
30	19.854359952550737	25.68505297313075	35.08122886123466	19.379358213083844
31	25.528246396757975	24.242228825596808	31.059581407899046	19.16994336974617
32	25.407077678651735	23.431891810250466	31.663897661647383	19.497132849450413
33	24.61099580661201	25.036613656766697	30.428621612494293	19.923768924127
34	20.233478882871935	24.94836753153526	32.366811989933154	22.45134159565965
35	20.70848062233883	24.795633853250088	32.33185294801454	22.164032576396536
36	25.526040243627186	25.443394353266207	28.60650883055217	20.424056572554434
37	20.348368549759783	29.028053782619246	29.79664359256764	20.82693407505333
38	20.95217569124717	29.651546598196727	27.998459086890186	21.397818623665913
39	21.026675785432943	27.96044537140588	28.558652278022812	22.45422656513837
40	22.578280252723324	25.382979698300073	29.10849351984944	22.93024652912716
41	19.367818335168966	23.40898175850769	30.2441532699432	26.979046636380144
42	21.31873651913159	24.446722250411955	27.464570029240015	26.769971201216443
43	20.87020861723413	25.0098004110233	29.4148093968547	24.70518157488787
44	20.22635131121863	26.617237522931266	29.87708332979783	23.279327836052275
45	19.203205370794947	31.119826358778198	27.139756406753545	22.537211863673313
46	21.339101009569614	28.24588764571217	28.36451080251366	22.050500542204556
47	20.714250561296268	26.372015117240068	29.455877785904715	23.457856535558946
48	21.022093775084386	24.72266109584717	31.880270372551383	22.37497475651706
49	20.71000795912168	24.797330894119924	31.009518702238907	23.48314244451949
50	19.604385832424	28.487206857402747	30.699299631233018	21.20910767894023
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	298.0
2	484.0
3	5026.0
4	9568.0
5	7628.0
6	5688.0
7	5647.0
8	5606.0
9	5924.5
10	6243.0
11	6373.5
12	6504.0
13	6297.5
14	6091.0
15	5954.5
16	5818.0
17	5487.5
18	5157.0
19	4882.0
20	4607.0
21	4437.0
22	4267.0
23	4296.0
24	4325.0
25	4366.5
26	4408.0
27	4939.0
28	5470.0
29	6637.5
30	7805.0
31	9337.5
32	10870.0
33	11947.0
34	13024.0
35	14497.0
36	15970.0
37	17003.0
38	18036.0
39	21312.0
40	24588.0
41	30504.0
42	36420.0
43	40177.0
44	43934.0
45	45683.0
46	47432.0
47	46847.0
48	46262.0
49	44941.0
50	43620.0
51	41255.5
52	38891.0
53	35868.5
54	32846.0
55	30583.5
56	28321.0
57	26466.0
58	24611.0
59	22933.5
60	21256.0
61	19661.0
62	18066.0
63	15984.5
64	13903.0
65	11745.0
66	9587.0
67	8148.0
68	6709.0
69	5841.5
70	4974.0
71	4101.5
72	3229.0
73	2557.0
74	1885.0
75	1561.0
76	1237.0
77	959.0
78	681.0
79	526.0
80	371.0
81	279.0
82	187.0
83	139.5
84	92.0
85	70.5
86	49.0
87	35.0
88	21.0
89	18.0
90	15.0
91	13.0
92	11.0
93	7.0
94	3.0
95	3.0
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	589261.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.58455105743085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.20014076687629	40.56888115435194
2	7.822528389501835	7.913981734339158
3	3.3149528267089563	5.030562015469017
4	1.8759545087661165	3.7957726652038883
5	1.2230504744301838	3.0933729584814325
6	0.8992180203486139	2.729192391725181
7	0.6728025642502496	2.3823390964021005
8	0.5272191880675745	2.133531674980916
9	0.4314003143163524	1.9639972103154515
>10	2.976318995159588	27.42183647813084
>50	0.05168245890519319	1.6341428437089665
>100	0.004367531694480533	0.3174022350919517
>500	0.0	0.0
>1k	0.0	0.0
>5k	3.639609745400443E-4	1.0149875417992629
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5513	0.9355786315401835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024946500786578445	0.0
2	0.0	0.0	0.0	0.10708327888660543	0.0
3	0.0	0.0	0.0	0.15256397419819062	0.0
4	0.0	0.0	0.0	0.1978749654227923	0.0
5	0.0	0.0	0.0	0.3268500715302727	0.0
6	0.0	0.0	0.0	0.43681831989559805	0.0
7	0.0	0.0	0.0	0.5208218429524438	0.0
8	0.0	0.0	0.0	0.7373642579434241	0.0
9	0.0	0.0	0.0	0.8850068136190924	0.0
10	0.0	0.0	0.0	1.081693850433	0.0
11	0.0	0.0	0.0	1.2410459881105316	0.0
12	0.0	0.0	0.0	1.3830883089157435	0.0
13	0.0	0.0	0.0	1.4440120761428297	0.0
14	0.0	0.0	0.0	1.4706556177992434	0.0
15	0.0	0.0	0.0	1.5039176188480148	0.0
16	0.0	0.0	0.0	1.5502468345945175	0.0
17	0.0	0.0	0.0	1.631874500433594	0.0
18	0.0	0.0	0.0	1.7384486670592487	0.0
19	0.0	0.0	0.0	1.7840990664578176	0.0
20	0.0	0.0	0.0	1.8387437824665132	0.0
21	0.0	0.0	0.0	1.8967825802148792	0.0
22	0.0	0.0	0.0	1.9594033883118007	0.0
23	0.0	0.0	0.0	2.0259273904093433	0.0
24	0.0	0.0	0.0	2.08159033093994	0.0
25	0.0	0.0	0.0	2.125713393555657	0.0
26	0.0	0.0	0.0	2.16915763982344	0.0
27	0.0	0.0	0.0	2.2277055498327565	0.0
28	0.0	0.0	0.0	2.283538194450337	0.0
29	0.0	0.0	0.0	2.3429346248945713	0.0
30	0.0	0.0	0.0	2.4308413419520383	0.0
31	0.0	0.0	0.0	2.499401793093383	0.0
32	0.0	0.0	0.0	2.577635377192789	0.0
33	0.0	0.0	0.0	2.646195828334134	0.0
34	0.0	0.0	0.0	2.7230717797376713	0.0
35	0.0	0.0	0.0	2.8136937621868747	0.0
36	0.0	0.0	0.0	2.887175631850742	0.0
37	0.0	0.0	0.0	2.963881879167296	0.0
38	0.0	0.0	0.0	3.0578979433561697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	20	7.8533526E-4	44.000004	15
ACCGTAT	20	7.8533526E-4	44.000004	26
TCTATAC	20	7.8533526E-4	44.000004	11
GTCTACG	30	2.5256086E-6	44.0	1
TACGGGT	55	1.8189894E-12	44.0	4
TCTAGCG	35	1.4444777E-7	44.0	1
CTACGGG	90	0.0	44.0	3
CGGTCTA	75	0.0	44.0	31
CGTTTTT	5645	0.0	43.142605	1
TACGGGA	80	0.0	41.250004	4
CGTTTGG	120	0.0	40.333332	2
CGGGATA	50	1.3460522E-9	39.600002	6
GCGTAAG	50	1.3460522E-9	39.600002	1
CGGGGTA	45	2.3475877E-8	39.11111	6
GTTTTTT	6485	0.0	38.40247	2
TGATTGG	150	0.0	38.13333	2
AACTTAA	710	0.0	38.112675	44
CACGGGT	35	7.2841685E-6	37.714287	4
TCGATTG	35	7.2841685E-6	37.714287	1
AAATTCG	35	7.2841685E-6	37.714287	30
>>END_MODULE