FastQCFastQC Report
Thu 26 May 2016
SRR1546013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546013_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences379225
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61871.6314852660030323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCGG650.044.0000042
AACGTAG351.4426587E-744.01
CTAGAAT207.849802E-444.041
CGGGTAG254.437181E-544.06
CCGGGTA207.849802E-444.017
TTGTGCG254.437181E-544.01
CGACGGC207.849802E-444.014
TAGACCC254.437181E-544.023
GCGCGAC351.4426587E-744.09
TTCACGG302.523213E-644.02
ATGCTCG207.849802E-444.01
TTCGTAG254.437181E-544.01
TATTGCG302.523213E-644.01
TACGCGG207.849802E-444.02
TACCTCT207.849802E-444.015
TTGCGAG254.437181E-544.01
TTACGCG207.849802E-444.01
ATAACGG207.849802E-444.02
CAGTCGA207.849802E-444.017
AATAGCG351.4426587E-744.01
Produced by FastQC (version 0.11.5)