##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546013_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	379225
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.075569912321182	31.0	28.0	33.0	25.0	33.0
2	28.835327312281628	31.0	28.0	33.0	16.0	33.0
3	28.254880348078316	31.0	27.0	33.0	16.0	34.0
4	32.28271870261718	35.0	32.0	35.0	26.0	37.0
5	33.70146746654361	35.0	33.0	35.0	28.0	37.0
6	33.48852791878173	35.0	33.0	37.0	28.0	37.0
7	34.46782253279715	35.0	35.0	37.0	32.0	37.0
8	34.82542817588503	35.0	35.0	37.0	32.0	37.0
9	36.56474915946997	39.0	37.0	39.0	32.0	39.0
10	36.243628452765506	38.0	35.0	39.0	31.0	39.0
11	36.335742633001516	38.0	35.0	39.0	32.0	39.0
12	35.10340299294614	37.0	34.0	39.0	29.0	39.0
13	35.3914378007779	37.0	35.0	39.0	30.0	39.0
14	36.07010350056035	38.0	35.0	40.0	29.0	41.0
15	36.62297580591997	38.0	35.0	40.0	31.0	41.0
16	36.145109104093876	38.0	34.0	40.0	31.0	41.0
17	35.77097237787593	37.0	34.0	40.0	30.0	41.0
18	35.42497198233239	37.0	34.0	39.0	29.0	40.0
19	34.85359087612895	37.0	33.0	39.0	27.0	40.0
20	35.35979431735777	36.0	34.0	39.0	30.0	41.0
21	35.623442547300414	36.0	34.0	39.0	30.0	40.0
22	35.96622321840596	37.0	34.0	40.0	31.0	41.0
23	36.29609334827609	37.0	35.0	40.0	32.0	41.0
24	35.99096051156965	37.0	35.0	40.0	31.0	41.0
25	35.653638341354075	36.0	34.0	40.0	30.0	41.0
26	35.764474915947	37.0	34.0	40.0	31.0	41.0
27	36.036189597204825	37.0	35.0	40.0	31.0	41.0
28	35.96323554617971	37.0	35.0	40.0	31.0	41.0
29	36.13272331729185	37.0	35.0	40.0	31.0	41.0
30	35.78370096908168	37.0	35.0	40.0	30.0	41.0
31	35.10599512162964	36.0	34.0	40.0	29.0	41.0
32	35.07120574856616	36.0	34.0	40.0	29.0	41.0
33	34.769780473333775	37.0	34.0	40.0	26.0	41.0
34	34.51118728986749	37.0	34.0	40.0	23.0	41.0
35	34.2750557057156	37.0	34.0	40.0	22.0	41.0
36	33.85392049574791	36.0	33.0	40.0	20.0	41.0
37	33.777635968092824	36.0	33.0	40.0	20.0	41.0
38	33.627701232777376	36.0	33.0	40.0	18.0	41.0
39	33.44467532467532	36.0	33.0	40.0	18.0	41.0
40	33.30233238842376	36.0	33.0	40.0	18.0	40.0
41	33.03563583624497	35.0	33.0	39.0	18.0	40.0
42	33.18755356318808	36.0	33.0	40.0	18.0	40.0
43	32.96391851803019	35.0	33.0	39.0	17.0	40.0
44	32.82013580328301	35.0	32.0	39.0	15.0	40.0
45	32.76203836772365	35.0	32.0	39.0	17.0	40.0
46	32.575281165534975	35.0	32.0	39.0	16.0	40.0
47	32.54526204759707	35.0	32.0	39.0	17.0	40.0
48	32.586113784692465	35.0	32.0	39.0	17.0	40.0
49	32.58272529500956	35.0	32.0	39.0	17.0	40.0
50	32.72326191574923	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	7.0
14	19.0
15	40.0
16	100.0
17	218.0
18	361.0
19	693.0
20	1021.0
21	1528.0
22	2214.0
23	3083.0
24	4414.0
25	6585.0
26	9497.0
27	11134.0
28	10869.0
29	10566.0
30	11784.0
31	14310.0
32	18666.0
33	28935.0
34	45954.0
35	36136.0
36	37554.0
37	52421.0
38	58408.0
39	12703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.075219197046607	23.02168897092755	28.212275034610062	21.69081679741578
2	36.3633726679412	20.044037181093017	30.634319994726084	12.9582701562397
3	27.751598655151955	19.009031577559497	39.60551124002901	13.633858527259543
4	25.160524754433382	25.32797152086492	35.672226250906455	13.839277473795239
5	20.196716988595163	30.069220119981544	36.103896103896105	13.630166787527193
6	18.778034148592525	36.41347485002308	34.31683037774408	10.491660623640318
7	77.97666293097765	4.598061836640517	14.952337003098423	2.472938229283407
8	79.02432592787922	3.3223020634188147	14.743490012525545	2.9098819961764124
9	75.49027622124069	4.590678357175819	16.207792207792206	3.7112532137912853
10	44.306414397784955	22.246687322829455	21.43714153866438	12.009756740721206
11	37.690816797415785	20.821412090447623	28.073307403256642	13.414463708879953
12	34.011734458434965	20.541367262179445	31.333113586920692	14.113784692464895
13	20.64025314786736	33.04423495286439	31.57307666952337	14.742435229744874
14	15.774276484936383	33.20851737095392	34.690750873491986	16.326455270617707
15	14.846331333640977	21.296855428835123	47.99156173775463	15.865251499769267
16	16.67928011075219	17.480123936976728	46.0204364163755	19.820159535895577
17	17.29764651592063	18.72845935790098	34.81547893730635	29.158415188872045
18	21.141802360076472	20.84883644274507	38.497725624629176	19.511635572549277
19	25.177137583228955	22.52936910804931	32.18537807370295	20.10811523501879
20	26.675720218867426	21.621464829586657	32.033489353286306	19.669325598259608
21	21.4893532863076	25.53813698991364	33.81053464302195	19.161975080756807
22	21.85140747577296	22.11352099676973	31.81277605643088	24.222295471026438
23	18.934933087217352	25.8698661744347	32.13738545718241	23.057815281165535
24	18.267255587052542	21.09354604786077	42.2414134089261	18.39778495616059
25	17.59746852132639	22.728723053596152	38.857933944228364	20.8158744808491
26	17.38150174698398	28.092029797613556	33.75726811259806	20.769200342804403
27	17.272068033489354	28.61309249126508	34.69787065726152	19.416968817984046
28	16.12314588964335	25.065594304172983	40.66583163029864	18.14542817588503
29	16.973037115169095	22.10112729909684	39.74764321972444	21.178192366009625
30	19.401410771969147	25.5753180829323	36.48599116619421	18.537279978904344
31	25.964269233304766	23.776386050497724	31.488166655679343	18.771178060518164
32	26.71923000857011	23.348407937240427	32.111279583360805	17.821082470828664
33	25.471685674731358	24.22071329685543	30.668864130793068	19.638736897620145
34	19.489748829850353	25.078251697541038	32.79820686927286	22.63379260333575
35	20.313006790164152	24.3085239633463	33.724042455006916	21.654426791482628
36	26.063682510383018	24.149779154855295	30.203968620212272	19.58256971454941
37	20.2299426461863	29.091963873689764	30.9001252554552	19.777968224668733
38	20.33410244577757	29.115696486254862	28.794251433845343	21.755949634122224
39	20.06513283670644	28.662667281956622	29.069286043905336	22.202913837431602
40	22.646713692398972	24.962225591667217	28.97119124530292	23.419869470630893
41	18.4869141011273	23.348407937240427	30.369569516777638	27.795108444854637
42	21.369899136396597	24.04403718109302	28.001845869866177	26.584217812644205
43	21.103566484277145	24.751532731228163	29.518887204166393	24.6260135803283
44	20.03322565759114	27.159601819500296	29.715604192761553	23.091568330147012
45	18.282549937372274	31.421715340497062	27.950161513613292	22.345573208517372
46	21.25914694442613	28.444063550662534	29.34142000131848	20.955369503592856
47	20.440635506625355	26.043905333245437	30.10930186564704	23.40615729448217
48	21.079570176016876	23.597072977783636	32.43457050563649	22.88878634056299
49	20.824049047399303	23.434900125255457	33.2971191245303	22.44393170281495
50	19.428835124266598	28.180895246885097	31.118465291054125	21.271804337794187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	151.0
1	307.0
2	463.0
3	5063.0
4	9663.0
5	6853.5
6	4044.0
7	4050.0
8	4056.0
9	4286.0
10	4516.0
11	4527.0
12	4538.0
13	4223.0
14	3908.0
15	3670.5
16	3433.0
17	3133.0
18	2833.0
19	2663.5
20	2494.0
21	2406.5
22	2319.0
23	2343.0
24	2367.0
25	2372.0
26	2377.0
27	2858.5
28	3340.0
29	3385.0
30	3430.0
31	4460.5
32	5491.0
33	6583.0
34	7675.0
35	8288.5
36	8902.0
37	10046.0
38	11190.0
39	13816.0
40	16442.0
41	21659.0
42	26876.0
43	30563.0
44	34250.0
45	34315.5
46	34381.0
47	32254.0
48	30127.0
49	28644.5
50	27162.0
51	25928.0
52	24694.0
53	22484.0
54	20274.0
55	18453.5
56	16633.0
57	15817.5
58	15002.0
59	13658.0
60	12314.0
61	11195.0
62	10076.0
63	8997.5
64	7919.0
65	6666.0
66	5413.0
67	4539.5
68	3666.0
69	3076.5
70	2487.0
71	2127.5
72	1768.0
73	1425.0
74	1082.0
75	887.5
76	693.0
77	539.0
78	385.0
79	289.5
80	194.0
81	159.5
82	125.0
83	78.0
84	31.0
85	24.0
86	17.0
87	13.0
88	9.0
89	6.0
90	3.0
91	3.5
92	4.0
93	5.0
94	6.0
95	3.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	379225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.78279700425638
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58422385795751	26.330493450250525
2	8.524203908070556	6.100397161307552
3	4.011376292083766	4.306147907019602
4	2.4826541580231503	3.553452390732617
5	1.7245902820831316	3.085533198964697
6	1.209418576575664	2.596582765127021
7	0.9457368168362587	2.3688775975412413
8	0.7899206812942385	2.261245711057251
9	0.6152084292318456	1.9812490504659503
>10	5.94100890037986	41.793477702374915
>50	0.16134387701553735	3.5372882055653783
>100	0.009577490416447423	0.45422176018699856
>500	0.0	0.0
>1k	0.0	0.0
>5k	7.367300320344171E-4	1.6310330994062447
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6187	1.6314852660030323	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6369569516777636E-4	0.0	0.0	0.05036587777704529	0.0
2	2.6369569516777636E-4	0.0	0.0	0.16480980947986024	0.0
3	2.6369569516777636E-4	0.0	0.0	0.21728525281824773	0.0
4	2.6369569516777636E-4	0.0	0.0	0.32540048783703607	0.0
5	2.6369569516777636E-4	0.0	0.0	0.5632540048783704	0.0
6	2.6369569516777636E-4	0.0	0.0	0.8208846990572879	0.0
7	2.6369569516777636E-4	0.0	0.0	1.0263036455929857	0.0
8	2.6369569516777636E-4	0.0	0.0	1.5993143911925638	0.0
9	5.273913903355527E-4	0.0	0.0	1.8825235678027556	0.0
10	5.273913903355527E-4	0.0	0.0	2.3426725558705255	0.0
11	5.273913903355527E-4	0.0	0.0	2.628782385127563	0.0
12	0.0010547827806711054	0.0	0.0	2.911727866042587	0.0
13	0.0013184784758388818	0.0	0.0	3.0172061441096973	0.0
14	0.0013184784758388818	0.0	0.0	3.0549146285186892	0.0
15	0.0013184784758388818	0.0	0.0	3.103962027819896	0.0
16	0.0013184784758388818	0.0	0.0	3.2044300876788188	0.0
17	0.0013184784758388818	0.0	0.0	3.3331135869206934	0.0
18	0.0013184784758388818	0.0	0.0	3.5398510119322304	0.0
19	0.0013184784758388818	0.0	0.0	3.6221240688245766	0.0
20	0.0015821741710066583	0.0	0.0	3.7265475641110157	0.0
21	0.0015821741710066583	0.0	0.0	3.849166062364032	0.0
22	0.0015821741710066583	0.0	0.0	3.972839343397719	0.0
23	0.0015821741710066583	0.0	0.0	4.110224800580131	0.0
24	0.0015821741710066583	0.0	0.0	4.2080559034873755	0.0
25	0.0015821741710066583	0.0	0.0	4.287692003428044	0.0
26	0.0015821741710066583	0.0	0.0	4.365745929197706	0.0
27	0.0015821741710066583	0.0	0.0	4.453292899993408	0.0
28	0.0015821741710066583	0.0	0.0	4.531083130067902	0.0
29	0.0015821741710066583	0.0	0.0	4.62733205880414	0.0
30	0.0015821741710066583	0.0	0.0	4.7633990375107125	0.0
31	0.0015821741710066583	0.0	0.0	4.8622849231986285	0.0
32	0.0015821741710066583	0.0	0.0	4.955896894983189	0.0
33	0.0015821741710066583	0.0	0.0	5.057947129013119	0.0
34	0.0015821741710066583	0.0	0.0	5.154723449139693	0.0
35	0.0015821741710066583	0.0	0.0	5.292108906322104	0.0
36	0.0015821741710066583	0.0	0.0	5.391258487705188	0.0
37	0.0015821741710066583	0.0	0.0	5.49541828729646	0.0
38	0.0015821741710066583	0.0	0.0	5.616718307073637	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGCGG	65	0.0	44.000004	2
AACGTAG	35	1.4426587E-7	44.0	1
CTAGAAT	20	7.849802E-4	44.0	41
CGGGTAG	25	4.437181E-5	44.0	6
CCGGGTA	20	7.849802E-4	44.0	17
TTGTGCG	25	4.437181E-5	44.0	1
CGACGGC	20	7.849802E-4	44.0	14
TAGACCC	25	4.437181E-5	44.0	23
GCGCGAC	35	1.4426587E-7	44.0	9
TTCACGG	30	2.523213E-6	44.0	2
ATGCTCG	20	7.849802E-4	44.0	1
TTCGTAG	25	4.437181E-5	44.0	1
TATTGCG	30	2.523213E-6	44.0	1
TACGCGG	20	7.849802E-4	44.0	2
TACCTCT	20	7.849802E-4	44.0	15
TTGCGAG	25	4.437181E-5	44.0	1
TTACGCG	20	7.849802E-4	44.0	1
ATAACGG	20	7.849802E-4	44.0	2
CAGTCGA	20	7.849802E-4	44.0	17
AATAGCG	35	1.4426587E-7	44.0	1
>>END_MODULE