FastQCFastQC Report
Thu 26 May 2016
SRR1546012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546012_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences380878
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64841.7023823901616792No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT351.4426769E-744.06
CCTTACG207.849846E-444.042
ACACGCG302.523244E-644.036
ATCGTAA207.849846E-444.014
GCGATCA351.4426769E-744.09
GCGATAT351.4426769E-744.09
CCGGTAC254.4372166E-544.020
TTGCACG207.849846E-444.01
GGCGATA1000.044.08
CGTTTTT41650.042.9963951
TCATGCA1100.042.024
GCCAGTA1000.041.80000325
CACGGGA900.041.5555574
TCGTTGA750.041.06666624
TGGGCGA2000.040.76
ATAGGGC1300.040.6153874
TAGGGCG1200.040.3333325
ACTCATG1150.040.17391622
ACGGGAA557.6397555E-1140.05
CATAGTT1100.040.036
Produced by FastQC (version 0.11.5)