##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546012_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	380878
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.0496852010355	31.0	28.0	33.0	25.0	33.0
2	28.773413008889985	31.0	28.0	33.0	16.0	33.0
3	28.246380730837696	31.0	27.0	33.0	16.0	34.0
4	32.291208733505215	35.0	32.0	35.0	26.0	37.0
5	33.74415167061369	35.0	33.0	35.0	30.0	37.0
6	33.58966388187294	35.0	33.0	37.0	28.0	37.0
7	34.53272176392441	35.0	35.0	37.0	32.0	37.0
8	34.88086998986552	35.0	35.0	37.0	32.0	37.0
9	36.68030445444473	39.0	37.0	39.0	32.0	39.0
10	36.256226403205225	38.0	35.0	39.0	31.0	39.0
11	36.39360372612752	38.0	35.0	39.0	32.0	39.0
12	35.035168741696815	37.0	34.0	39.0	29.0	39.0
13	35.390579135576225	37.0	35.0	39.0	30.0	39.0
14	36.08554182704173	38.0	35.0	40.0	30.0	41.0
15	36.66283691890842	38.0	35.0	40.0	31.0	41.0
16	36.178954940952224	38.0	34.0	40.0	31.0	41.0
17	35.82071686996886	37.0	34.0	40.0	30.0	41.0
18	35.51005308786541	37.0	34.0	39.0	29.0	40.0
19	34.92936320816639	37.0	33.0	39.0	27.0	41.0
20	35.33785621642626	36.0	34.0	39.0	29.0	41.0
21	35.52067066094655	36.0	34.0	39.0	30.0	40.0
22	35.93817180304455	37.0	34.0	40.0	31.0	41.0
23	36.3189604020185	37.0	35.0	40.0	32.0	41.0
24	35.87249985559681	36.0	35.0	40.0	31.0	41.0
25	35.56876480132746	36.0	34.0	40.0	30.0	41.0
26	35.728907944276116	36.0	34.0	40.0	31.0	41.0
27	36.05677408514013	37.0	35.0	40.0	31.0	41.0
28	35.990080813278794	37.0	35.0	40.0	31.0	41.0
29	36.151636482023115	37.0	35.0	40.0	31.0	41.0
30	35.832613067701466	37.0	35.0	40.0	31.0	41.0
31	35.1511743918	36.0	34.0	40.0	29.0	41.0
32	35.166181822000745	37.0	34.0	40.0	29.0	41.0
33	34.90608803868955	37.0	34.0	40.0	27.0	41.0
34	34.564343438056284	37.0	34.0	40.0	24.0	41.0
35	34.361672241505154	37.0	34.0	40.0	23.0	41.0
36	33.914571070001415	36.0	33.0	40.0	21.0	41.0
37	33.82497545145689	36.0	33.0	40.0	20.0	41.0
38	33.706507070505516	36.0	33.0	40.0	19.0	41.0
39	33.50529565897742	36.0	33.0	40.0	18.0	41.0
40	33.34514726500349	36.0	33.0	40.0	18.0	40.0
41	33.06613928869612	35.0	33.0	39.0	18.0	40.0
42	33.21735568869822	36.0	33.0	40.0	18.0	40.0
43	33.011552255577904	35.0	33.0	39.0	17.0	40.0
44	32.841534559622765	35.0	32.0	39.0	16.0	40.0
45	32.78313265665121	35.0	32.0	39.0	17.0	40.0
46	32.5923497812948	35.0	32.0	39.0	17.0	40.0
47	32.562337546405935	35.0	32.0	39.0	17.0	40.0
48	32.59392771438623	35.0	32.0	39.0	17.0	40.0
49	32.65284421783353	35.0	32.0	39.0	18.0	40.0
50	32.80882854877415	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	7.0
14	17.0
15	47.0
16	89.0
17	166.0
18	340.0
19	632.0
20	1003.0
21	1470.0
22	2124.0
23	2929.0
24	4255.0
25	6575.0
26	9610.0
27	11039.0
28	10720.0
29	10527.0
30	11537.0
31	14028.0
32	19122.0
33	28952.0
34	46594.0
35	36547.0
36	38464.0
37	54153.0
38	58631.0
39	11292.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.243106716586414	22.823843855512788	28.123966204401405	21.809083223499385
2	36.34418370186779	20.236401157325968	30.383744926196837	13.035670214609402
3	27.553442309610954	18.982981427123647	39.69039955051224	13.773176712753166
4	25.155299072143837	25.44463056411764	35.623743035827744	13.776327327910776
5	20.178902430699594	30.003833248441758	36.16879945809419	13.648464862764456
6	18.76322601988038	36.56446421163732	34.32884020605023	10.343469562432064
7	77.81284295758746	4.57863147779604	15.095909976422897	2.512615588193595
8	78.9733195406403	3.2984315187540365	14.812092060974905	2.916156879630748
9	75.43727912874989	4.609349975582733	16.262687789790956	3.6906831058764222
10	44.232536402732634	22.0330394509528	21.548107267944065	12.186316878370501
11	37.999044313402194	20.635479077289844	27.99426588041315	13.371210728894816
12	34.151880654697834	20.002205430610324	31.344157446741477	14.501756467950367
13	20.692452701389946	32.99192917417126	31.534769663776853	14.780848460661943
14	15.68586266468528	33.33377091877189	34.60530668613047	16.375059730412364
15	14.724925041614375	21.32651400185886	48.18524566921691	15.763315287309847
16	16.548606115344022	17.473836766628683	46.221362220973646	19.75619489705365
17	17.161138212235937	18.6925997300973	34.82112382442672	29.32513823324004
18	21.14955445050646	20.787233707381368	38.6291673449241	19.434044497188076
19	25.161600302459053	22.556304118379114	32.127347864670575	20.154747714491254
20	26.80779672231003	21.457264530899657	31.974543029526515	19.760395717263794
21	21.489033233738887	25.52208318674221	33.680076034845804	19.308807544673098
22	21.626084993094903	21.947185187907937	32.0152384753123	24.411491343684855
23	18.984294183439314	25.865237687658514	32.051995652151085	23.098472476751088
24	18.223945725402885	21.051097726831163	42.42592116110671	18.299035386659245
25	17.688866251135536	22.488040789964238	39.029820572466775	20.79327238643345
26	17.228876438124544	28.15442215092497	33.854672624829995	20.76202878612049
27	17.234652565913493	28.66324649887891	34.62368527454986	19.478415660657745
28	16.105419583173614	25.047127951732577	40.76738483188843	18.08006763320538
29	16.90567583320643	22.027788425690115	39.96082735154039	21.105708389563063
30	19.238443806153153	25.695629571673866	36.53374571385063	18.53218090832235
31	26.169272050367837	23.817862937738592	31.183476073703392	18.82938893819018
32	26.84481645041194	23.168836215271032	32.20296262845321	17.78338470586382
33	25.47876222832508	24.043657024033944	30.81301624142114	19.66456450621984
34	19.600501998015112	24.93685642121624	32.67975572230478	22.782885858463864
35	20.22826206816881	24.27942805832839	33.85047180461985	21.641838068882947
36	26.080529723428498	24.020815064141274	30.215712117791	19.682943094639228
37	20.43882818120238	29.091992711576935	30.797000614369956	19.67217849285073
38	20.4128356061521	29.265801647771728	28.5847436712018	21.73661907487437
39	20.01822105766151	28.685563356245307	29.00561334600581	22.290602240087377
40	22.810978843619218	24.77144912544174	29.008501409900283	23.409070621038758
41	18.519840998955043	23.470245065349012	30.281349933574532	27.728564002121413
42	21.40134111185209	23.9625286837255	28.072243605563983	26.563886598858428
43	21.28319304344173	24.658289531030935	29.35690693607927	24.701610489448065
44	20.139782292492608	27.20713719353704	29.908527139924068	22.744553374046284
45	18.207404995825435	31.440776311574837	27.979563009677637	22.37225568292209
46	21.33281523217408	28.50387788215649	29.257925109877704	20.905381775791724
47	20.464033102463254	25.99099974269976	30.12854509843047	23.416422056406514
48	20.92822373568439	23.60335855575801	32.612280047679306	22.856137660878286
49	20.83265507590357	23.271231207893344	33.44903092328777	22.447082792915314
50	19.444021445187172	28.30171340954321	31.13490409002358	21.119361055246035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	317.5
2	486.0
3	5208.5
4	9931.0
5	6971.0
6	4011.0
7	4080.0
8	4149.0
9	4350.5
10	4552.0
11	4510.5
12	4469.0
13	4190.5
14	3912.0
15	3645.5
16	3379.0
17	3155.0
18	2931.0
19	2670.0
20	2409.0
21	2356.0
22	2303.0
23	2296.5
24	2290.0
25	2342.0
26	2394.0
27	2911.5
28	3429.0
29	3419.5
30	3410.0
31	4462.0
32	5514.0
33	6586.0
34	7658.0
35	8292.5
36	8927.0
37	10043.0
38	11159.0
39	13748.5
40	16338.0
41	21866.0
42	27394.0
43	30626.0
44	33858.0
45	34251.0
46	34644.0
47	32315.0
48	29986.0
49	28575.5
50	27165.0
51	26036.0
52	24907.0
53	22602.0
54	20297.0
55	18546.5
56	16796.0
57	15918.0
58	15040.0
59	13817.5
60	12595.0
61	11401.0
62	10207.0
63	9138.5
64	8070.0
65	6788.0
66	5506.0
67	4648.0
68	3790.0
69	3166.5
70	2543.0
71	2174.0
72	1805.0
73	1412.5
74	1020.0
75	872.0
76	724.0
77	553.0
78	382.0
79	284.0
80	186.0
81	143.5
82	101.0
83	64.0
84	27.0
85	21.5
86	16.0
87	11.5
88	7.0
89	6.5
90	6.0
91	3.0
92	0.0
93	1.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	380878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.6542787196363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1593714390987	26.08444620243025
2	8.88365160202449	6.334803805334493
3	4.084047730510889	4.3684132826379996
4	2.4939492119588467	3.5567984126319208
5	1.7274424244836213	3.079535683733166
6	1.228623852150578	2.6283418359701956
7	0.9446733673008948	2.357715327573442
8	0.7266957311745887	2.0727849714934945
9	0.673494584431688	2.1611667266543724
>10	5.899544732643376	41.49725452062448
>50	0.16670331931504845	3.6469514259152893
>100	0.011064379600556314	0.5065283367933113
>500	0.0	0.0
>1k	0.0	0.0
>5k	7.376253067037542E-4	1.7052594682075963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6484	1.7023823901616792	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6255126313412693E-4	0.0	0.0	0.0438460609433992	0.0
2	2.6255126313412693E-4	0.0	0.0	0.15779330914361028	0.0
3	2.6255126313412693E-4	0.0	0.0	0.21187886934924044	0.0
4	2.6255126313412693E-4	0.0	0.0	0.31007304176140393	0.0
5	2.6255126313412693E-4	0.0	0.0	0.5650103182646412	0.0
6	2.6255126313412693E-4	0.0	0.0	0.8223105561360856	0.0
7	2.6255126313412693E-4	0.0	0.0	1.0171235933816078	0.0
8	2.6255126313412693E-4	0.0	0.0	1.5879100394351997	0.0
9	2.6255126313412693E-4	0.0	0.0	1.8869559281449704	0.0
10	2.6255126313412693E-4	0.0	0.0	2.3401194083144734	0.0
11	2.6255126313412693E-4	0.0	0.0	2.595844338607113	0.0
12	2.6255126313412693E-4	0.0	0.0	2.867059793424666	0.0
13	5.251025262682539E-4	0.0	0.0	2.9723428499414513	0.0
14	5.251025262682539E-4	0.0	0.0	3.0117255394115703	0.0
15	5.251025262682539E-4	0.0	0.0	3.06449834330153	0.0
16	5.251025262682539E-4	0.0	0.0	3.1794957965542774	0.0
17	5.251025262682539E-4	0.0	0.0	3.311559081910743	0.0
18	5.251025262682539E-4	0.0	0.0	3.518449477260435	0.0
19	5.251025262682539E-4	0.0	0.0	3.596952304937539	0.0
20	5.251025262682539E-4	0.0	0.0	3.6888452470344837	0.0
21	5.251025262682539E-4	0.0	0.0	3.799379328813951	0.0
22	5.251025262682539E-4	0.0	0.0	3.917527397224308	0.0
23	5.251025262682539E-4	0.0	0.0	4.0530038490015174	0.0
24	5.251025262682539E-4	0.0	0.0	4.1506729188874125	0.0
25	5.251025262682539E-4	0.0	0.0	4.2325889129852605	0.0
26	5.251025262682539E-4	0.0	0.0	4.3066283691890845	0.0
27	5.251025262682539E-4	0.0	0.0	4.390644773392005	0.0
28	5.251025262682539E-4	0.0	0.0	4.465734434648365	0.0
29	5.251025262682539E-4	0.0	0.0	4.565503914639334	0.0
30	5.251025262682539E-4	0.0	0.0	4.700717815153409	0.0
31	5.251025262682539E-4	0.0	0.0	4.79812433377617	0.0
32	5.251025262682539E-4	0.0	0.0	4.895268301135797	0.0
33	5.251025262682539E-4	0.0	0.0	4.988211448285278	0.0
34	5.251025262682539E-4	0.0	0.0	5.087455825749978	0.0
35	5.251025262682539E-4	0.0	0.0	5.214793188370029	0.0
36	5.251025262682539E-4	0.0	0.0	5.306686130466973	0.0
37	5.251025262682539E-4	0.0	0.0	5.405930507931673	0.0
38	5.251025262682539E-4	0.0	0.0	5.52407857634203	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	35	1.4426769E-7	44.0	6
CCTTACG	20	7.849846E-4	44.0	42
ACACGCG	30	2.523244E-6	44.0	36
ATCGTAA	20	7.849846E-4	44.0	14
GCGATCA	35	1.4426769E-7	44.0	9
GCGATAT	35	1.4426769E-7	44.0	9
CCGGTAC	25	4.4372166E-5	44.0	20
TTGCACG	20	7.849846E-4	44.0	1
GGCGATA	100	0.0	44.0	8
CGTTTTT	4165	0.0	42.996395	1
TCATGCA	110	0.0	42.0	24
GCCAGTA	100	0.0	41.800003	25
CACGGGA	90	0.0	41.555557	4
TCGTTGA	75	0.0	41.066666	24
TGGGCGA	200	0.0	40.7	6
ATAGGGC	130	0.0	40.615387	4
TAGGGCG	120	0.0	40.333332	5
ACTCATG	115	0.0	40.173916	22
ACGGGAA	55	7.6397555E-11	40.0	5
CATAGTT	110	0.0	40.0	36
>>END_MODULE