FastQCFastQC Report
Thu 26 May 2016
SRR1546011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546011_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences375057
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62441.6648136149971873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG551.8189894E-1244.0000042
TCACGAC502.7284841E-1144.025
AGCCGAA207.8496913E-444.040
GATGTTA254.437087E-544.011
CACGGGA750.044.04
AACCCGT207.8496913E-444.032
TACGGGT302.5231402E-644.04
TATGCGA207.8496913E-444.040
GCGTTAG351.4426041E-744.01
ACGGGAA800.044.05
GCCCTTA207.8496913E-444.014
CGGGCTA254.437087E-544.06
CTGTTAG254.437087E-544.01
GTCGTAG207.8496913E-444.01
CGTACAT254.437087E-544.035
CGGTCTA502.7284841E-1144.031
TTACCGG351.4426041E-744.02
GATTACG254.437087E-544.01
ATGCAAG207.8496913E-444.01
CTAGCGG254.437087E-544.02
Produced by FastQC (version 0.11.5)