##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546011_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	375057
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.930434573944762	31.0	28.0	33.0	23.0	33.0
2	28.683568097649157	31.0	28.0	33.0	16.0	33.0
3	28.10657046795554	31.0	26.0	33.0	16.0	34.0
4	32.145569873379245	35.0	32.0	35.0	25.0	37.0
5	33.66390708612291	35.0	33.0	35.0	28.0	37.0
6	33.508645352573076	35.0	33.0	37.0	28.0	37.0
7	34.453733699144394	35.0	35.0	37.0	32.0	37.0
8	34.84653799289175	35.0	35.0	37.0	32.0	37.0
9	36.67144993960918	39.0	37.0	39.0	32.0	39.0
10	36.33073372847327	38.0	35.0	39.0	32.0	39.0
11	36.40498377579941	38.0	35.0	39.0	32.0	39.0
12	35.21310894077433	37.0	34.0	39.0	30.0	39.0
13	35.472290878453144	37.0	35.0	39.0	30.0	39.0
14	36.159354444791056	38.0	35.0	40.0	30.0	41.0
15	36.63458887582421	38.0	35.0	40.0	31.0	41.0
16	36.140754605299996	38.0	34.0	40.0	31.0	41.0
17	35.773874371095594	37.0	34.0	40.0	30.0	41.0
18	35.439402544146624	37.0	34.0	39.0	29.0	40.0
19	34.83894714670037	37.0	33.0	39.0	27.0	41.0
20	35.43529650159842	36.0	34.0	39.0	30.0	41.0
21	35.604254819934035	36.0	34.0	39.0	30.0	40.0
22	35.95718250825875	37.0	34.0	40.0	31.0	41.0
23	36.311592637919034	37.0	35.0	40.0	32.0	41.0
24	35.86956116003701	36.0	35.0	40.0	31.0	41.0
25	35.58399389959393	36.0	34.0	40.0	30.0	41.0
26	35.74228450608841	37.0	34.0	40.0	31.0	41.0
27	36.05330389780753	37.0	35.0	40.0	31.0	41.0
28	35.99966405106424	37.0	35.0	40.0	31.0	41.0
29	36.14849476212949	37.0	35.0	40.0	31.0	41.0
30	35.82189640507976	37.0	35.0	40.0	31.0	41.0
31	35.123413241187336	36.0	34.0	40.0	29.0	41.0
32	35.1416478028673	37.0	34.0	40.0	29.0	41.0
33	34.902345510149125	37.0	34.0	40.0	27.0	41.0
34	34.56789501329131	37.0	34.0	40.0	24.0	41.0
35	34.32394542696177	37.0	34.0	40.0	23.0	41.0
36	33.930869174552136	36.0	33.0	40.0	21.0	41.0
37	33.85741100686029	36.0	33.0	40.0	20.0	41.0
38	33.73273395777175	36.0	33.0	40.0	19.0	41.0
39	33.48038831430956	36.0	33.0	40.0	18.0	41.0
40	33.3404069248141	36.0	33.0	40.0	18.0	41.0
41	33.04803003276835	35.0	33.0	39.0	18.0	40.0
42	33.249639388146335	36.0	33.0	40.0	18.0	41.0
43	33.04075380542157	35.0	33.0	40.0	17.0	40.0
44	32.896234439031936	35.0	33.0	39.0	16.0	40.0
45	32.84170139472133	35.0	32.0	39.0	17.0	40.0
46	32.64302492687778	35.0	32.0	39.0	17.0	40.0
47	32.628205846044736	35.0	32.0	39.0	17.0	40.0
48	32.65236750680563	35.0	32.0	39.0	17.0	40.0
49	32.71567255110556	35.0	32.0	39.0	18.0	40.0
50	32.79951847319207	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	2.0
13	7.0
14	18.0
15	37.0
16	87.0
17	205.0
18	334.0
19	639.0
20	1040.0
21	1521.0
22	2015.0
23	2933.0
24	4250.0
25	6494.0
26	9273.0
27	11074.0
28	10644.0
29	10525.0
30	11450.0
31	13821.0
32	18603.0
33	28845.0
34	45815.0
35	35559.0
36	36644.0
37	52382.0
38	58079.0
39	12758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.287585620319042	22.847993771613382	28.061068050989586	21.80335255707799
2	36.469656612194946	19.99722708814927	30.58495108743471	12.948165212221074
3	27.63766574147396	19.09603073666136	39.69290001253143	13.573403509333248
4	25.096985258240746	25.27402501486441	35.759897828863345	13.869091898031499
5	20.211327878162518	29.973577349576196	36.20009758516706	13.614997187094227
6	18.670762044169287	36.45499217452281	34.37664141717126	10.49760436413665
7	77.75991382643171	4.6392948271862675	15.070242656449553	2.5305486899324636
8	78.96639710763964	3.2824344033040314	14.802816638537609	2.948351850518721
9	75.463729513114	4.588902486822003	16.313520344907573	3.6338476551564165
10	44.392452347243214	22.160898210138726	21.509797177495688	11.936852265122369
11	37.89797284146143	20.62086562842448	28.11012726065638	13.37103426945771
12	34.09695059684261	20.434760583058043	31.345368837270065	14.122919982829277
13	20.669124959672796	33.033112300263696	31.631458684946555	14.666304055116955
14	15.785600588710516	33.25334549148529	34.662997890987235	16.298056028816955
15	14.818814206907216	21.3583535302634	48.09855568620237	15.72427657662702
16	16.700128247173097	17.397622228087997	46.2492367826757	19.653012742063204
17	17.145927152406166	18.638233655151083	34.871232905931635	29.344606286511116
18	21.097059913559807	20.849630856109872	38.6455925366011	19.40771669372922
19	25.395339908333938	22.502446294829852	32.15671217974867	19.945501617087537
20	26.837520696854078	21.531127268655165	31.957275827407567	19.67407620708319
21	21.459404837131423	25.601441914162383	33.65035181319106	19.288801435515136
22	21.7254977243459	22.064646173781586	32.024732240699414	24.185123861173103
23	19.004044718536118	25.795012491434637	32.128716435101865	23.072226354927384
24	18.33694611752347	21.034669396918336	42.3290326537033	18.29935183185489
25	17.580527759780512	22.58936641630472	39.089791684997216	20.74031413891755
26	17.20378502467625	28.20797905385048	33.85725369743799	20.73098222403528
27	17.236846665973438	28.728699904281218	34.716323118885924	19.318130310859416
28	16.079689220571804	25.131113404095913	40.80899703245107	17.980200342881215
29	16.828375420269452	22.013987207277825	40.14883071106525	21.008806661387467
30	19.200014931063812	25.723823312189882	36.540312539160716	18.535849217585593
31	26.072037050368348	23.673734925624636	31.344035706572598	18.910192317434415
32	26.741801912775927	23.278328360755832	32.192706708580296	17.78716301788795
33	25.653967263642592	23.989153648645406	30.80465102637732	19.552228061334677
34	19.649279976110297	24.965005319191484	32.756087741329985	22.629626963368235
35	20.244122893320217	24.255513161999374	34.07508725340415	21.42527669127626
36	26.0208448315856	24.06860823821446	30.351119963098945	19.559426967101
37	20.347307209304187	29.217425617972737	30.81051680144618	19.6247503712769
38	20.399565932644904	29.236089447737278	28.694038506147066	21.67030611347075
39	19.994027574475346	28.80522160631584	29.045185131859956	22.155565687348858
40	22.773605078694708	24.98526890579299	28.976128961731153	23.26499705378116
41	18.483590494244876	23.47323206872555	30.29326209082886	27.74991534620071
42	21.380750125980853	24.051544165286877	27.954150969052705	26.613554739679568
43	21.20371036935719	24.689580517094733	29.4187283532903	24.687980760257773
44	19.96789821280499	27.37637212477037	29.835998261597567	22.819731400827074
45	18.233495175400005	31.58959838104608	27.959483491842573	22.21742295171134
46	21.206376630752125	28.781491879900923	29.267551332197506	20.744580157149446
47	20.372636692556064	26.17122197425991	30.114889203507733	23.341252129676292
48	21.003207512458104	23.665202889160845	32.6003780758658	22.731211522515242
49	20.8810927405701	23.392710974598526	33.4365176493173	22.28967863551407
50	19.44557760553729	28.36422197159365	31.18619303199247	21.004007390876588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161.0
1	312.5
2	464.0
3	5178.0
4	9892.0
5	6929.5
6	3967.0
7	4088.5
8	4210.0
9	4337.0
10	4464.0
11	4448.0
12	4432.0
13	4133.0
14	3834.0
15	3583.5
16	3333.0
17	3081.0
18	2829.0
19	2665.0
20	2501.0
21	2412.0
22	2323.0
23	2303.5
24	2284.0
25	2300.0
26	2316.0
27	2828.0
28	3340.0
29	3388.5
30	3437.0
31	4478.0
32	5519.0
33	6589.5
34	7660.0
35	8240.0
36	8820.0
37	9969.0
38	11118.0
39	13694.5
40	16271.0
41	21493.5
42	26716.0
43	30127.5
44	33539.0
45	33829.0
46	34119.0
47	31762.0
48	29405.0
49	28127.0
50	26849.0
51	25450.5
52	24052.0
53	22012.5
54	19973.0
55	18182.5
56	16392.0
57	15644.0
58	14896.0
59	13513.0
60	12130.0
61	11086.5
62	10043.0
63	8999.0
64	7955.0
65	6710.5
66	5466.0
67	4586.0
68	3706.0
69	3119.5
70	2533.0
71	2118.5
72	1704.0
73	1370.5
74	1037.0
75	869.0
76	701.0
77	517.5
78	334.0
79	257.0
80	180.0
81	133.5
82	87.0
83	61.0
84	35.0
85	22.0
86	9.0
87	11.5
88	14.0
89	8.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	375057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.44417134832879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06825002678262	25.898435740718135
2	8.69396808472915	6.163009889840838
3	4.1270794007791585	4.388427283481238
4	2.544607819663355	3.6076606229778116
5	1.7191746172698683	3.046735985610539
6	1.282170879714538	2.7267290615063726
7	0.9925446029185455	2.4625944683692724
8	0.8016753888968475	2.273177587983839
9	0.6049755629684652	1.929857176386536
>10	5.980225680109438	41.58077560278861
>50	0.17628754903785226	3.852892067523893
>100	0.008287021535967415	0.4024054574647243
>500	0.0	0.0
>1k	0.0	0.0
>5k	7.533655941788558E-4	1.6672990553482006
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6244	1.6648136149971873	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6662613949346366E-4	0.0	0.0	0.045326443713888824	0.0
2	2.6662613949346366E-4	0.0	0.0	0.16477495420696053	0.0
3	2.6662613949346366E-4	0.0	0.0	0.21490066843173172	0.0
4	2.6662613949346366E-4	0.0	0.0	0.3215511242291172	0.0
5	2.6662613949346366E-4	0.0	0.0	0.5649807895866495	0.0
6	2.6662613949346366E-4	0.0	0.0	0.8049443151307668	0.0
7	2.6662613949346366E-4	0.0	0.0	0.9857168377073352	0.0
8	2.6662613949346366E-4	0.0	0.0	1.5477647397595566	0.0
9	2.6662613949346366E-4	0.0	0.0	1.810924739439605	0.0
10	2.6662613949346366E-4	0.0	0.0	2.237526562629147	0.0
11	2.6662613949346366E-4	0.0	0.0	2.497487048635274	0.0
12	2.6662613949346366E-4	0.0	0.0	2.761713552873297	0.0
13	7.99878418480391E-4	0.0	0.0	2.8622316074623324	0.0
14	7.99878418480391E-4	0.0	0.0	2.8984927624334436	0.0
15	7.99878418480391E-4	0.0	0.0	2.943285953868345	0.0
16	7.99878418480391E-4	0.0	0.0	3.0427375038994073	0.0
17	7.99878418480391E-4	0.0	0.0	3.173650938390698	0.0
18	7.99878418480391E-4	0.0	0.0	3.372020786173835	0.0
19	7.99878418480391E-4	0.0	0.0	3.450408871184913	0.0
20	7.99878418480391E-4	0.0	0.0	3.550393673494962	0.0
21	7.99878418480391E-4	0.0	0.0	3.6514449803629847	0.0
22	7.99878418480391E-4	0.0	0.0	3.7594285668578378	0.0
23	7.99878418480391E-4	0.0	0.0	3.8834097217222983	0.0
24	7.99878418480391E-4	0.0	0.0	3.9735293568710888	0.0
25	7.99878418480391E-4	0.0	0.0	4.047651423650271	0.0
26	7.99878418480391E-4	0.0	0.0	4.116707593779079	0.0
27	0.0010665045579738546	0.0	0.0	4.197495314045598	0.0
28	0.0010665045579738546	0.0	0.0	4.275083520638196	0.0
29	0.0010665045579738546	0.0	0.0	4.364403277368507	0.0
30	0.0010665045579738546	0.0	0.0	4.483051909443098	0.0
31	0.0010665045579738546	0.0	0.0	4.588902486822003	0.0
32	0.0010665045579738546	0.0	0.0	4.674222851459912	0.0
33	0.0010665045579738546	0.0	0.0	4.76567561730617	0.0
34	0.0013331306974673184	0.0	0.0	4.854728747896987	0.0
35	0.0013331306974673184	0.0	0.0	4.988841696062199	0.0
36	0.0013331306974673184	0.0	0.0	5.0888264983722475	0.0
37	0.0013331306974673184	0.0	0.0	5.181612394915973	0.0
38	0.0013331306974673184	0.0	0.0	5.288262850713358	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGG	55	1.8189894E-12	44.000004	2
TCACGAC	50	2.7284841E-11	44.0	25
AGCCGAA	20	7.8496913E-4	44.0	40
GATGTTA	25	4.437087E-5	44.0	11
CACGGGA	75	0.0	44.0	4
AACCCGT	20	7.8496913E-4	44.0	32
TACGGGT	30	2.5231402E-6	44.0	4
TATGCGA	20	7.8496913E-4	44.0	40
GCGTTAG	35	1.4426041E-7	44.0	1
ACGGGAA	80	0.0	44.0	5
GCCCTTA	20	7.8496913E-4	44.0	14
CGGGCTA	25	4.437087E-5	44.0	6
CTGTTAG	25	4.437087E-5	44.0	1
GTCGTAG	20	7.8496913E-4	44.0	1
CGTACAT	25	4.437087E-5	44.0	35
CGGTCTA	50	2.7284841E-11	44.0	31
TTACCGG	35	1.4426041E-7	44.0	2
GATTACG	25	4.437087E-5	44.0	1
ATGCAAG	20	7.8496913E-4	44.0	1
CTAGCGG	25	4.437087E-5	44.0	2
>>END_MODULE