FastQCFastQC Report
Thu 26 May 2016
SRR1546010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546010_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences326415
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.9711563500451879No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT5580.17094802628555675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA207.8481884E-444.00000438
GATCATC207.8481884E-444.00000415
ATCGTAG207.8481884E-444.0000041
TAACCGC207.8481884E-444.00000427
TATAGCG207.8481884E-444.0000041
CGTCGCA207.8481884E-444.00000422
GCGCTCA207.8481884E-444.00000423
CCCGCCA207.8481884E-444.00000439
GCACGGC254.4358138E-544.03
TTGTATC502.7284841E-1144.042
CACGACG351.441822E-744.026
ACGGCAA254.4358138E-544.05
CGAATTT254.4358138E-544.024
AGCGATT502.7284841E-1144.022
TCGTAAG302.522127E-644.01
CTCGAAT254.4358138E-544.022
CATCGAC351.441822E-744.017
CGGTCTA454.783942E-1044.031
TAGGCGA351.441822E-744.05
TCGAATT254.4358138E-544.023
Produced by FastQC (version 0.11.5)