##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546010_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	326415
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.803391388263407	28.0	25.0	30.0	16.0	33.0
2	25.986357857328862	30.0	25.0	31.0	10.0	33.0
3	26.315242865677128	30.0	25.0	31.0	16.0	33.0
4	28.22655208859887	32.0	19.0	35.0	10.0	37.0
5	31.24234793131443	33.0	30.0	35.0	19.0	37.0
6	29.744187613927057	33.0	28.0	35.0	17.0	37.0
7	29.636340241716834	32.0	28.0	35.0	17.0	35.0
8	32.38449519783098	33.0	32.0	35.0	28.0	35.0
9	26.939282814821624	30.0	23.0	34.0	10.0	35.0
10	31.214086362452704	34.0	28.0	37.0	17.0	37.0
11	33.56717062634989	35.0	32.0	37.0	27.0	39.0
12	33.01377694039796	35.0	31.0	37.0	23.0	39.0
13	33.27387221788214	35.0	32.0	38.0	24.0	39.0
14	32.913343443162844	35.0	31.0	38.0	22.0	40.0
15	31.858808571909993	34.0	27.0	38.0	18.0	40.0
16	30.401219306710782	33.0	27.0	37.0	17.0	40.0
17	30.54240767121609	32.0	26.0	37.0	17.0	40.0
18	31.443913423096365	34.0	27.0	38.0	18.0	39.0
19	31.006880811237227	34.0	27.0	37.0	18.0	39.0
20	31.80321676393548	34.0	29.0	37.0	19.0	39.0
21	32.7738921311827	34.0	31.0	37.0	24.0	39.0
22	33.19631144402065	35.0	31.0	37.0	25.0	39.0
23	34.510923211249484	35.0	33.0	38.0	29.0	40.0
24	34.17304045463597	35.0	33.0	38.0	27.0	40.0
25	32.95870594182253	35.0	31.0	38.0	23.0	40.0
26	26.68616638328508	33.0	21.0	36.0	0.0	39.0
27	29.934659865508632	34.0	24.0	37.0	15.0	39.0
28	31.417912779743578	34.0	29.0	37.0	20.0	39.0
29	32.750820274803544	35.0	30.0	37.0	23.0	39.0
30	32.48923915873964	35.0	31.0	38.0	23.0	39.0
31	31.342199347456457	34.0	29.0	37.0	20.0	39.0
32	31.69478118346277	34.0	30.0	37.0	20.0	39.0
33	31.45512001593064	34.0	30.0	37.0	18.0	39.0
34	31.56114149165939	34.0	30.0	38.0	18.0	39.0
35	30.836956022241626	34.0	29.0	37.0	15.0	39.0
36	30.547667846146776	34.0	28.0	37.0	12.0	39.0
37	30.586648897875403	34.0	28.0	37.0	12.0	39.0
38	30.334610848153424	34.0	28.0	37.0	11.0	39.0
39	30.241085734417844	34.0	28.0	37.0	10.0	39.0
40	30.20830231453824	34.0	28.0	37.0	11.0	39.0
41	29.671586783695602	33.0	27.0	36.0	10.0	38.0
42	29.843505966331204	33.0	27.0	36.0	10.0	38.0
43	29.379477046091633	33.0	26.0	36.0	10.0	38.0
44	29.146613973009817	33.0	26.0	36.0	10.0	38.0
45	28.628647580533983	32.0	24.0	35.0	10.0	38.0
46	28.15356524669516	32.0	23.0	35.0	9.0	38.0
47	28.25699799335202	32.0	24.0	35.0	10.0	38.0
48	28.366046290764825	32.0	24.0	35.0	10.0	38.0
49	28.207597690057135	32.0	24.0	35.0	9.0	38.0
50	30.076568172418547	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	10.0
12	16.0
13	40.0
14	105.0
15	197.0
16	420.0
17	767.0
18	1322.0
19	2067.0
20	3065.0
21	4356.0
22	5804.0
23	7755.0
24	10094.0
25	12852.0
26	15544.0
27	17227.0
28	19341.0
29	22944.0
30	27866.0
31	32485.0
32	36281.0
33	36438.0
34	31766.0
35	23758.0
36	11544.0
37	2285.0
38	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.42131948593049	23.993995373987104	27.7438230473477	22.84086209273471
2	35.74222998330347	17.593554217790235	32.48655852212674	14.17765727677956
3	29.04860377127277	14.79558231086194	41.17764195885606	14.978171959009238
4	26.32722148185592	25.317157606114915	34.5578481381064	13.797772773922768
5	19.449780187797742	30.432424980469648	36.66896435519201	13.4488304765406
6	18.54173368258199	35.02780203115666	35.51184841382902	10.918615872432333
7	80.03339307323499	3.355237963941608	14.776281727249055	1.8350872355743457
8	81.84672885743609	2.074353200680116	14.187767106291071	1.8911508355927271
9	69.56543051024003	2.9140817670756554	14.493819217866827	13.026668504817488
10	45.5591807974511	21.975399414855325	21.24320267144586	11.222217116247721
11	39.23502290029563	20.257953831778565	27.85932019055497	12.647703077370831
12	35.0575188027511	20.372531899575694	31.344454145795996	13.225495151877212
13	20.226398909363848	34.05664568111147	31.523673850772788	14.193281558751897
14	15.17944947382933	33.842501110549456	35.070998575433116	15.907050840188102
15	13.489269794586644	19.793208032719082	48.15832605731967	18.559196115374597
16	14.969593921847954	15.679426496944075	46.189053811865264	23.16192576934271
17	16.453900709219855	17.777062941347673	35.357137386455896	30.41189896297658
18	20.84340486803609	20.246312209916823	39.593155951779174	19.31712697026791
19	26.037100010722547	21.843665272735628	32.5992984391036	19.51993627743823
20	27.622505093209565	21.007613007980638	32.303662515509394	19.0662193833004
21	21.291913668183142	25.417030467349843	34.03183064503776	19.259225219429254
22	21.423647810302835	21.65311030436714	32.00955838426543	24.913683501064597
23	18.337086224591395	26.080296554999006	32.511067199730405	23.071550020679197
24	17.43271602101619	20.56615045264464	44.34998391618032	17.651149610158846
25	17.25992984391036	21.37217958733514	40.6782776526814	20.689612916073095
26	13.73251841980301	40.29441048971402	28.088169967679182	17.884901122803793
27	16.270085627192376	29.873627131106105	34.96377311091708	18.89251413078443
28	15.462831058621692	24.838012958963283	42.14205842256023	17.557097559854785
29	16.24649602499885	21.68221435902149	41.29926627146424	20.772023344515418
30	18.736884028001164	25.981036410704167	36.94744420446364	18.33463535683103
31	26.951273685339217	23.403029885268754	31.5337836802843	18.11191274910773
32	27.27999632369836	22.844844752845304	32.274864819325096	17.600294104131244
33	26.113076911293902	23.803440405618616	30.733575356524668	19.349907326562814
34	19.256161634728798	24.40329028996829	32.530674141813336	23.809873933489577
35	20.104774596755664	23.881255457010248	34.0180445138857	21.99592543234839
36	26.378689704823614	23.529249574927622	30.079806381446932	20.01225433880183
37	19.802398786820458	29.71493344362238	30.40607815204571	20.076589617511452
38	19.96507513441478	30.192546298423785	28.355620912029167	21.486757655132273
39	19.642173306986503	29.249574927622813	28.714673038922843	22.39357872646784
40	22.95911646217239	24.582816353415133	28.6521759110335	23.805891273378982
41	18.0521728474488	22.767336059923718	30.315396044912152	28.865095047715332
42	20.8060291346905	23.39659635739779	27.116400900693904	28.68097360721781
43	20.739855705160608	24.120215063645972	29.082915919917895	26.057013311275522
44	19.23532925876568	27.02388064274007	30.000153179235024	23.740636919259224
45	17.581912595928493	32.588575892651996	27.30542407671216	22.52408743470735
46	20.488335401253003	28.665655683715514	28.879493895807485	21.966515019223994
47	19.89338725242406	25.81223289370893	29.475667478516616	24.818712375350398
48	20.204341099520548	23.228405557342647	33.18321768301089	23.384035660125914
49	20.247537643797006	23.206347747499347	33.43596342079868	23.110151187904968
50	18.99177427507927	29.019499716618412	30.955685247307873	21.03304076099444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	210.5
2	326.0
3	2876.5
4	5427.0
5	4417.0
6	3407.0
7	3351.5
8	3296.0
9	3582.5
10	3869.0
11	4013.5
12	4158.0
13	4128.5
14	4099.0
15	3873.5
16	3648.0
17	3480.5
18	3313.0
19	3133.0
20	2953.0
21	2773.0
22	2593.0
23	2556.0
24	2519.0
25	2448.5
26	2378.0
27	2736.0
28	3094.0
29	3289.0
30	3484.0
31	4176.5
32	4869.0
33	5870.0
34	6871.0
35	7258.5
36	7646.0
37	8547.5
38	9449.0
39	11628.0
40	13807.0
41	18007.0
42	22207.0
43	25164.0
44	28121.0
45	28470.0
46	28819.0
47	27248.0
48	25677.0
49	24483.5
50	23290.0
51	22179.0
52	21068.0
53	19252.5
54	17437.0
55	15994.5
56	14552.0
57	13653.5
58	12755.0
59	11822.0
60	10889.0
61	9928.5
62	8968.0
63	7931.0
64	6894.0
65	5823.5
66	4753.0
67	4083.0
68	3413.0
69	2848.0
70	2283.0
71	1975.0
72	1667.0
73	1327.0
74	987.0
75	816.5
76	646.0
77	479.5
78	313.0
79	247.5
80	182.0
81	138.5
82	95.0
83	73.0
84	51.0
85	37.0
86	23.0
87	17.5
88	12.0
89	10.0
90	8.0
91	5.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	326415.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.75325493722042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.03242158342925	43.27477248908186
2	6.976812183048112	7.360991034828847
3	2.8239497796722888	4.4691762797087895
4	1.6921342737517653	3.570623629249408
5	1.0781524248498047	2.8438024864642064
6	0.8636963299743885	2.733767561008834
7	0.6496770626555894	2.3990805799194383
8	0.544384736663118	2.2974453437696853
9	0.4218337384966604	2.0027792473231587
>10	2.885179592000183	26.783863178019168
>50	0.028022025404847495	0.8925363415458824
>100	0.0024908467026531103	0.14651130229277276
>500	6.227116756632776E-4	0.18330337820485917
>1k	6.227116756632776E-4	1.0413471485831605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3170	0.9711563500451879	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	558	0.17094802628555675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03492486558522127	0.0
2	0.0	0.0	0.0	0.12652604812891566	0.0
3	0.0	0.0	0.0	0.1672717246450071	0.0
4	0.0	0.0	0.0	0.23528330499517486	0.0
5	0.0	0.0	0.0	0.4062313312807316	0.0
6	0.0	0.0	0.0	0.5615550755939525	0.0
7	0.0	0.0	0.0	0.6801158035016773	0.0
8	0.0	0.0	0.0	1.0305898932340731	0.0
9	0.0	0.0	0.0	1.2508616331970037	0.0
10	0.0	0.0	0.0	1.5657981404040868	0.0
11	0.0	0.0	0.0	1.7545149579523	0.0
12	0.0	0.0	0.0	1.9380236815097345	0.0
13	0.0	0.0	0.0	2.0106306389105892	0.0
14	0.0	0.0	0.0	2.035752033454345	0.0
15	0.0	0.0	0.0	2.0645497296386504	0.0
16	0.0	0.0	0.0	2.123370555887444	0.0
17	0.0	0.0	0.0	2.2192607570117793	0.0
18	0.0	0.0	0.0	2.346399522080787	0.0
19	0.0	0.0	0.0	2.3895960663572446	0.0
20	0.0	0.0	0.0	2.4478041756659468	0.0
21	0.0	0.0	0.0	2.516428472956206	0.0
22	0.0	0.0	0.0	2.585359128716511	0.0
23	0.0	0.0	0.0	2.6601105954076867	0.0
24	0.0	0.0	0.0	2.714336044605793	0.0
25	0.0	0.0	0.0	2.758145305822343	0.0
26	0.0	0.0	0.0	2.800422774688663	0.0
27	0.0	0.0	0.0	2.8537291484766327	0.0
28	0.0	0.0	0.0	2.9153072009558385	0.0
29	0.0	0.0	0.0	2.9778043288451816	0.0
30	0.0	0.0	0.0	3.0672610020985553	0.0
31	0.0	0.0	0.0	3.1441569780800513	0.0
32	0.0	0.0	0.0	3.210943124550036	0.0
33	0.0	0.0	0.0	3.2841627988909825	0.0
34	0.0	0.0	0.0	3.3521743792411502	0.0
35	0.0	0.0	0.0	3.4489836557756233	0.0
36	0.0	0.0	0.0	3.51852702847602	0.0
37	0.0	0.0	0.0	3.588989476586554	0.0
38	0.0	0.0	0.0	3.6664981695081416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	20	7.8481884E-4	44.000004	38
GATCATC	20	7.8481884E-4	44.000004	15
ATCGTAG	20	7.8481884E-4	44.000004	1
TAACCGC	20	7.8481884E-4	44.000004	27
TATAGCG	20	7.8481884E-4	44.000004	1
CGTCGCA	20	7.8481884E-4	44.000004	22
GCGCTCA	20	7.8481884E-4	44.000004	23
CCCGCCA	20	7.8481884E-4	44.000004	39
GCACGGC	25	4.4358138E-5	44.0	3
TTGTATC	50	2.7284841E-11	44.0	42
CACGACG	35	1.441822E-7	44.0	26
ACGGCAA	25	4.4358138E-5	44.0	5
CGAATTT	25	4.4358138E-5	44.0	24
AGCGATT	50	2.7284841E-11	44.0	22
TCGTAAG	30	2.522127E-6	44.0	1
CTCGAAT	25	4.4358138E-5	44.0	22
CATCGAC	35	1.441822E-7	44.0	17
CGGTCTA	45	4.783942E-10	44.0	31
TAGGCGA	35	1.441822E-7	44.0	5
TCGAATT	25	4.4358138E-5	44.0	23
>>END_MODULE