FastQCFastQC Report
Thu 26 May 2016
SRR1546009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546009_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences760353
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107531.4142115570004985No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9110.1198127711733892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG207.854799E-444.01
ACGCCTA207.854799E-444.017
GCGACTA254.4414148E-544.027
CGTTGAT551.8189894E-1244.025
CCGTGTA254.4414148E-544.032
ATTAACG207.854799E-444.010
ACGATCT207.854799E-444.036
CTAACGG254.4414148E-544.02
AAGCACG351.4452235E-744.01
TACTCCG207.854799E-444.035
TTGCACG254.4414148E-544.01
CGACTAT254.4414148E-544.028
TGTACCG302.5265817E-644.01
ACGAGCC207.854799E-444.023
ATACCTC302.5265817E-644.035
TACGTAG302.5265817E-644.01
CGTTTTT87600.043.2968061
TTACCGG800.041.252
ACGGGTA750.041.0666665
TATAGCG750.041.0666661
Produced by FastQC (version 0.11.5)