##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546009_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	760353
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.152986836377313	31.0	28.0	33.0	25.0	33.0
2	28.815689554719977	31.0	28.0	33.0	16.0	33.0
3	28.19114411332631	31.0	27.0	33.0	16.0	34.0
4	32.19904570640216	35.0	32.0	35.0	25.0	37.0
5	33.682665814430926	35.0	33.0	35.0	28.0	37.0
6	33.4770915614195	35.0	33.0	37.0	28.0	37.0
7	34.456987741220196	35.0	35.0	37.0	32.0	37.0
8	34.915991651246195	35.0	35.0	37.0	32.0	37.0
9	36.72023257618501	39.0	37.0	39.0	32.0	39.0
10	36.381816077532406	39.0	35.0	39.0	32.0	39.0
11	36.410743431011646	39.0	35.0	39.0	32.0	39.0
12	35.116044784461955	37.0	34.0	39.0	29.0	39.0
13	35.06996224122217	37.0	34.0	39.0	27.0	39.0
14	35.83508317847105	38.0	34.0	40.0	27.0	41.0
15	36.50391857466203	38.0	35.0	40.0	31.0	41.0
16	36.156500993617435	37.0	34.0	40.0	31.0	41.0
17	35.71571888320293	37.0	34.0	40.0	30.0	41.0
18	35.40372958349609	37.0	34.0	39.0	29.0	40.0
19	34.83740446871387	36.0	33.0	39.0	27.0	40.0
20	35.29115687055881	35.0	34.0	39.0	30.0	40.0
21	35.46570079949708	36.0	34.0	39.0	30.0	40.0
22	35.799758796243324	36.0	34.0	40.0	31.0	41.0
23	36.138579054728524	36.0	35.0	40.0	32.0	41.0
24	35.83364305789548	36.0	35.0	40.0	31.0	41.0
25	35.47445199795358	35.0	34.0	40.0	30.0	41.0
26	35.59103469046614	35.0	34.0	40.0	31.0	41.0
27	35.86569264538971	36.0	35.0	40.0	31.0	41.0
28	35.83307095520107	36.0	35.0	40.0	31.0	41.0
29	35.99159469351736	36.0	35.0	40.0	31.0	41.0
30	35.640348627545364	36.0	35.0	40.0	31.0	41.0
31	34.9761018895171	35.0	34.0	40.0	29.0	41.0
32	34.88476010484604	35.0	34.0	40.0	29.0	41.0
33	34.575873311475064	36.0	34.0	40.0	25.0	41.0
34	34.21699920957766	36.0	34.0	40.0	23.0	41.0
35	33.940094929591915	36.0	34.0	40.0	21.0	41.0
36	33.50978558643156	35.0	33.0	40.0	18.0	41.0
37	33.39011222419061	35.0	33.0	40.0	18.0	41.0
38	33.239480872699914	35.0	33.0	40.0	18.0	41.0
39	33.062574882981984	35.0	33.0	40.0	17.0	40.0
40	32.91101896092999	35.0	33.0	39.0	16.0	40.0
41	32.653791068096005	35.0	32.0	39.0	15.0	40.0
42	32.78789588520069	35.0	33.0	39.0	15.0	40.0
43	32.561710153047336	35.0	32.0	39.0	15.0	40.0
44	32.28155738190025	35.0	32.0	39.0	12.0	40.0
45	32.248805489029436	35.0	32.0	39.0	12.0	40.0
46	32.11616315053666	35.0	31.0	39.0	12.0	40.0
47	32.11113785307614	35.0	31.0	38.0	12.0	40.0
48	32.18524816762741	35.0	32.0	38.0	12.0	40.0
49	32.16042943211903	35.0	32.0	38.0	12.0	40.0
50	32.31161315862501	35.0	32.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	9.0
13	7.0
14	23.0
15	66.0
16	175.0
17	409.0
18	779.0
19	1386.0
20	2187.0
21	3104.0
22	4627.0
23	6484.0
24	9558.0
25	14572.0
26	20738.0
27	24134.0
28	22842.0
29	22019.0
30	23848.0
31	29030.0
32	39334.0
33	61999.0
34	101608.0
35	69581.0
36	69040.0
37	98510.0
38	109963.0
39	24316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.462051178860346	22.713134557238547	28.42640194751648	22.39841231638463
2	37.3359479084057	20.505870299716054	29.4284365288228	12.72974526305545
3	27.486969867942918	19.287094283839217	40.08822218101329	13.137713667204576
4	24.14891504340747	25.202504626140755	36.63587833545734	14.012701994994432
5	20.366592885146765	29.37359358087625	37.38434648117387	12.875467052803105
6	18.230611308168708	36.89233816398436	34.71229810364396	10.16475242420297
7	77.82740385057993	4.7364842382419745	15.446509713251608	1.9896021979264895
8	79.83975863842188	2.8072487384149207	15.017366933516406	2.3356256896467826
9	76.51788051076277	4.23987279592505	16.22522696694825	3.0170197263639387
10	46.18894118915819	23.002605368822113	21.020762724681823	9.787690717337869
11	41.24755212381617	19.32010526689577	28.30211756907647	11.130225040211586
12	36.9425779868035	20.008601268095212	30.446121735562297	12.60269900953899
13	19.4979174146745	35.94935510216965	31.156712737373297	13.39601474578255
14	14.584015582236146	37.014781292373414	34.13467165908467	14.266531466305782
15	13.604470555123738	21.2440800522915	51.19240668478982	13.959042707794932
16	15.634185700589068	17.390080659904015	49.36075743766382	17.614976201843092
17	15.814102134140326	17.858810315734928	35.10159097156189	31.22549657856285
18	20.29044404375336	21.08191852994596	39.98537521388092	18.64226221241976
19	26.00252777328425	22.480742497234836	32.424807951043796	19.091921778437122
20	27.487495939386047	22.23138463319011	32.094040531174336	18.187078896249506
21	20.887798167430127	25.48382133035577	34.72110980031643	18.907270701897673
22	20.655274589565636	22.14721320228894	33.05898707573982	24.138525132405604
23	17.90457853128744	27.38478049011446	32.071550976980426	22.63909000161767
24	17.78029415284743	22.27320731291913	43.865415142703455	16.081083391529987
25	16.229435538493306	22.550446963449872	41.21454114075962	20.0055763572972
26	15.624979450334253	30.70297611767166	34.45307640004051	19.21896803195358
27	16.627803138805266	29.793135556774285	35.62766241469423	17.95139888972622
28	14.641488887398351	25.605606869440905	43.528203347655634	16.224700895505116
29	15.385879979430605	22.241511508470406	41.98957589435433	20.383032617744654
30	17.64009611325266	27.239584771809938	37.34923121234479	17.771087902592612
31	26.545696538318385	23.934409412470263	31.33465640301281	18.185237646198544
32	27.178428966545802	23.12859947945231	32.37917125335206	17.313800300649827
33	25.155026678398063	25.44173561490518	30.720073439573458	18.6831642671233
34	18.235345951156898	25.821953750429078	32.8780184993023	23.064681799111728
35	19.38783696519906	24.694977201378833	34.27447514509708	21.642710688325028
36	27.646369515212015	24.07355530917876	29.740396894600273	18.539678281008953
37	19.965068856176014	30.14073726282398	31.08095844956224	18.813235431437768
38	20.61910717785029	30.77873040548272	28.00883273953019	20.593329677136804
39	20.09408787760422	29.39923956372895	29.946222346725797	20.560450211941035
40	23.25143716142371	25.319687040098483	29.03020044637162	22.398675352106192
41	18.380673187322206	23.322982877689704	30.988501393431733	27.307842541556354
42	21.924948017565526	23.835376463300598	27.84759184221013	26.392083676923743
43	21.036150314393446	25.40070204234086	28.7605888317663	24.802558811499395
44	19.453201342008253	28.55016025451336	30.199394228733233	21.79724417474515
45	17.282236014061887	34.0939011222419	26.758360919204634	21.865501944491573
46	21.278406213955886	29.941750739459174	28.806883118761945	19.972959927823
47	20.697491822877005	26.02738464897225	29.880594934195038	23.3945285939557
48	22.062121146362283	22.970646528651823	33.20352520474043	21.763707120245463
49	21.02825924274646	22.972356260842	33.53376655316676	22.46561794324478
50	19.548551791075987	29.408577331844548	30.018162616574145	21.024708260505317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	311.0
1	530.5
2	750.0
3	9040.0
4	17330.0
5	12878.0
6	8426.0
7	8705.5
8	8985.0
9	9653.5
10	10322.0
11	10239.0
12	10156.0
13	9676.5
14	9197.0
15	8502.5
16	7808.0
17	7454.5
18	7101.0
19	6447.5
20	5794.0
21	5632.5
22	5471.0
23	5047.5
24	4624.0
25	4628.0
26	4632.0
27	5733.0
28	6834.0
29	6968.5
30	7103.0
31	9016.5
32	10930.0
33	13637.0
34	16344.0
35	17909.5
36	19475.0
37	20568.0
38	21661.0
39	28627.5
40	35594.0
41	45874.5
42	56155.0
43	65627.5
44	75100.0
45	76297.0
46	77494.0
47	71117.5
48	64741.0
49	59678.0
50	54615.0
51	50536.5
52	46458.0
53	41870.0
54	37282.0
55	34174.5
56	31067.0
57	28045.5
58	25024.0
59	22308.0
60	19592.0
61	18371.0
62	17150.0
63	14828.5
64	12507.0
65	10433.5
66	8360.0
67	6777.0
68	5194.0
69	4517.5
70	3841.0
71	3329.5
72	2818.0
73	2371.0
74	1924.0
75	1468.0
76	1012.0
77	786.5
78	561.0
79	454.5
80	348.0
81	248.5
82	149.0
83	99.5
84	50.0
85	39.0
86	28.0
87	20.0
88	12.0
89	9.5
90	7.0
91	7.0
92	7.0
93	6.0
94	5.0
95	3.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	760353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.492326261567143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.02060358406264	22.420244435024358
2	7.3771981121882515	4.351414672417462
3	3.3780706463003676	2.988814849059403
4	2.103693586498495	2.4817127042951976
5	1.4445573261421571	2.130167798306078
6	1.0513613164388906	1.86042545779239
7	0.8654310293736112	1.786650199261931
8	0.6671149818130795	1.5739818158088619
9	0.5335578747719804	1.4162276629983266
>10	5.599120551029024	36.59285625608163
>50	0.8650150718818913	17.229751485756488
>100	0.09338230888823867	3.6307491426955614
>500	4.468053056853525E-4	0.12004545671961879
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.468053056853525E-4	1.4169580637827233
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10753	1.4142115570004985	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	911	0.1198127711733892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024988393548785893	0.0
2	0.0	0.0	0.0	0.09771777056183115	0.0
3	0.0	0.0	0.0	0.14361750397512735	0.0
4	0.0	0.0	0.0	0.24002009592912765	0.0
5	0.0	0.0	0.0	0.41138786852948567	0.0
6	0.0	0.0	0.0	0.6373355533548234	0.0
7	0.0	0.0	0.0	0.8058099330179535	0.0
8	0.0	0.0	0.0	1.3163622685778842	0.0
9	0.0	0.0	0.0	1.5811077223342316	0.0
10	0.0	0.0	0.0	1.9634301436306558	0.0
11	0.0	0.0	0.0	2.1929288106971367	0.0
12	0.0	0.0	0.0	2.40717140591278	0.0
13	1.3151786078308364E-4	0.0	0.0	2.490422211788472	0.0
14	2.630357215661673E-4	0.0	0.0	2.5214604269332797	0.0
15	2.630357215661673E-4	0.0	0.0	2.5576278386486275	0.0
16	2.630357215661673E-4	0.0	0.0	2.6485066804497386	0.0
17	2.630357215661673E-4	0.0	0.0	2.75477311196247	0.0
18	2.630357215661673E-4	0.0	0.0	2.93048097396867	0.0
19	2.630357215661673E-4	0.0	0.0	3.0037364224248475	0.0
20	2.630357215661673E-4	0.0	0.0	3.09553588925144	0.0
21	2.630357215661673E-4	0.0	0.0	3.196410088472065	0.0
22	2.630357215661673E-4	0.0	0.0	3.295837591224076	0.0
23	2.630357215661673E-4	0.0	0.0	3.4085483979151787	0.0
24	2.630357215661673E-4	0.0	0.0	3.4882482215497275	0.0
25	2.630357215661673E-4	0.0	0.0	3.5567690270177144	0.0
26	2.630357215661673E-4	0.0	0.0	3.6246322431817855	0.0
27	2.630357215661673E-4	0.0	0.0	3.697887691637963	0.0
28	2.630357215661673E-4	0.0	0.0	3.772063765119622	0.0
29	2.630357215661673E-4	0.0	0.0	3.851763588754171	0.0
30	2.630357215661673E-4	0.0	0.0	3.980914128043159	0.0
31	2.630357215661673E-4	0.0	0.0	4.080210112934387	0.0
32	2.630357215661673E-4	0.0	0.0	4.172930204786461	0.0
33	2.630357215661673E-4	0.0	0.0	4.259863510764079	0.0
34	2.630357215661673E-4	0.0	0.0	4.356529138439646	0.0
35	2.630357215661673E-4	0.0	0.0	4.483969945538454	0.0
36	2.630357215661673E-4	0.0	0.0	4.581819233961068	0.0
37	3.945535823492509E-4	0.0	0.0	4.677958790193502	0.0
38	3.945535823492509E-4	0.0	0.0	4.785671918174848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.854799E-4	44.0	1
ACGCCTA	20	7.854799E-4	44.0	17
GCGACTA	25	4.4414148E-5	44.0	27
CGTTGAT	55	1.8189894E-12	44.0	25
CCGTGTA	25	4.4414148E-5	44.0	32
ATTAACG	20	7.854799E-4	44.0	10
ACGATCT	20	7.854799E-4	44.0	36
CTAACGG	25	4.4414148E-5	44.0	2
AAGCACG	35	1.4452235E-7	44.0	1
TACTCCG	20	7.854799E-4	44.0	35
TTGCACG	25	4.4414148E-5	44.0	1
CGACTAT	25	4.4414148E-5	44.0	28
TGTACCG	30	2.5265817E-6	44.0	1
ACGAGCC	20	7.854799E-4	44.0	23
ATACCTC	30	2.5265817E-6	44.0	35
TACGTAG	30	2.5265817E-6	44.0	1
CGTTTTT	8760	0.0	43.296806	1
TTACCGG	80	0.0	41.25	2
ACGGGTA	75	0.0	41.066666	5
TATAGCG	75	0.0	41.066666	1
>>END_MODULE