FastQCFastQC Report
Thu 26 May 2016
SRR1546008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546008_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences759085
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109871.4474004887463194No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9050.11922248496545183No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACG254.4414068E-544.01
CGATTGA254.4414068E-544.010
CTAAGCG302.5265763E-644.01
CTACGCT302.5265763E-644.024
GGACGTA207.8547915E-444.08
AACGACT207.8547915E-444.041
CGGTCTA351.4452235E-744.031
CGGTAGT302.5265763E-644.012
CCACGTC207.8547915E-444.014
TCGACCA254.4414068E-544.029
TATCGTT207.8547915E-444.013
AATACGG302.5265763E-644.02
TACGTAG302.5265763E-644.01
TAGTACG207.8547915E-444.01
TATCCGG207.8547915E-444.02
CGTTTTT88400.043.5520321
TCGTTGA750.041.06666624
CGTTGAT750.041.06666625
TGTTGCG557.8216544E-1140.01
ACCCGCT2150.039.9069834
Produced by FastQC (version 0.11.5)