##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546008_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	759085
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.128706271366184	31.0	28.0	33.0	25.0	33.0
2	28.77861372573559	31.0	28.0	33.0	16.0	33.0
3	28.188065895123735	31.0	27.0	33.0	16.0	34.0
4	32.18377915516707	35.0	32.0	35.0	25.0	37.0
5	33.71497131414795	35.0	33.0	35.0	30.0	37.0
6	33.562658990758614	35.0	33.0	37.0	28.0	37.0
7	34.51484616347313	35.0	35.0	37.0	32.0	37.0
8	34.95264693677256	35.0	35.0	37.0	32.0	37.0
9	36.84100463057497	39.0	37.0	39.0	32.0	39.0
10	36.377634915720904	39.0	35.0	39.0	32.0	39.0
11	36.45375419090089	39.0	35.0	39.0	32.0	39.0
12	35.06867873821772	37.0	34.0	39.0	29.0	39.0
13	35.07757365775901	37.0	34.0	39.0	27.0	39.0
14	35.85571708043236	38.0	34.0	40.0	27.0	41.0
15	36.541905056745954	38.0	35.0	40.0	31.0	41.0
16	36.183295678349594	37.0	34.0	40.0	31.0	41.0
17	35.76101227135301	37.0	34.0	40.0	30.0	41.0
18	35.4643024167254	37.0	34.0	39.0	29.0	40.0
19	34.90547303661645	37.0	33.0	39.0	27.0	40.0
20	35.26184419399672	35.0	34.0	39.0	30.0	40.0
21	35.382467049144694	35.0	34.0	39.0	30.0	40.0
22	35.7769511978237	36.0	34.0	39.0	31.0	41.0
23	36.152971011151585	36.0	35.0	40.0	32.0	41.0
24	35.7414070887977	35.0	34.0	40.0	31.0	41.0
25	35.39331695396431	35.0	34.0	40.0	30.0	41.0
26	35.55143626866556	35.0	34.0	40.0	31.0	41.0
27	35.877098085194675	36.0	35.0	40.0	31.0	41.0
28	35.840356481816926	36.0	35.0	40.0	31.0	41.0
29	36.00426961407484	36.0	35.0	40.0	31.0	41.0
30	35.689830519638775	36.0	35.0	40.0	31.0	41.0
31	35.01634599550775	35.0	34.0	40.0	29.0	41.0
32	34.963135880698474	36.0	34.0	40.0	29.0	41.0
33	34.65409670853725	36.0	34.0	40.0	26.0	41.0
34	34.23102155885046	36.0	34.0	40.0	23.0	41.0
35	33.97824617796426	36.0	34.0	40.0	21.0	41.0
36	33.54289572314036	35.0	33.0	40.0	18.0	41.0
37	33.420983157353916	35.0	33.0	40.0	18.0	41.0
38	33.30943965432066	35.0	33.0	40.0	18.0	41.0
39	33.093000125150674	35.0	33.0	40.0	18.0	40.0
40	32.912924112582914	35.0	33.0	39.0	16.0	40.0
41	32.657145115500896	35.0	32.0	39.0	15.0	40.0
42	32.795246909107675	35.0	33.0	39.0	15.0	40.0
43	32.5806701489293	35.0	32.0	39.0	15.0	40.0
44	32.30074365848357	35.0	32.0	39.0	12.0	40.0
45	32.25041727869738	35.0	32.0	39.0	12.0	40.0
46	32.10567196032065	35.0	31.0	39.0	12.0	40.0
47	32.10576549398289	35.0	31.0	38.0	12.0	40.0
48	32.177421500885934	35.0	32.0	38.0	12.0	40.0
49	32.2083086874329	35.0	32.0	38.0	12.0	40.0
50	32.3766824532167	35.0	32.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	10.0
13	5.0
14	27.0
15	71.0
16	176.0
17	389.0
18	743.0
19	1288.0
20	2074.0
21	3123.0
22	4465.0
23	6371.0
24	9274.0
25	14272.0
26	20673.0
27	24101.0
28	22649.0
29	21950.0
30	23501.0
31	28834.0
32	38901.0
33	62342.0
34	102245.0
35	69643.0
36	69811.0
37	99737.0
38	110595.0
39	21813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.570146953239753	22.612619140148997	28.388915602337022	22.428318304274224
2	37.551262375096336	20.449620266505068	29.270503303319128	12.72861405507947
3	27.493758933452778	19.15319101286417	40.06652746398625	13.286522589696807
4	24.11205596211228	25.25119057813025	36.56033250558238	14.076420954175092
5	20.353583590770466	29.37299511912368	37.38184788264819	12.891573407457663
6	18.282405791182807	36.79258581054823	34.840762233478465	10.084246164790505
7	77.71593431565636	4.762312520995672	15.529222682571781	1.9925304807761977
8	79.78790254055869	2.8147045456042474	15.087901881870936	2.3094910319661173
9	76.60696759914897	4.207960900294434	16.27340811635061	2.9116633842059847
10	46.26253976827365	22.633960623645574	21.07076282629745	10.032736781783331
11	41.270081743151295	19.18375412503211	28.40656843436506	11.139595697451538
12	36.950934348590735	19.688572426012897	30.486177437309394	12.874315788086973
13	19.569876891257238	35.91020768425143	31.107846947311568	13.412068477179762
14	14.648557144456815	36.939209706422865	34.16046951263693	14.251763636483398
15	13.583063820257282	21.26560266636806	51.19716500787131	13.954168505503336
16	15.609714327117516	17.261439759710704	49.47245697122193	17.656388941949846
17	15.83037472746794	17.83199509936305	35.21832205879447	31.119308114374544
18	20.248325286364505	21.04625964154212	39.95547270727257	18.749942364820804
19	26.013555794146903	22.38813835077758	32.55103183437955	19.04727402069597
20	27.38757846617968	22.00675813644058	32.19033441577689	18.41532898160285
21	20.929144957415836	25.437204002186842	34.86737321907296	18.766277821324355
22	20.70334679251994	22.11649551762978	33.10867689389199	24.07148079595829
23	18.01300249642662	27.295493917018515	32.16174736689567	22.529756219659195
24	17.895756074747887	22.26693980252541	43.78455640672652	16.052747716000184
25	16.248246243833037	22.464677868749877	41.259806214060355	20.027269673356738
26	15.60813347648814	30.656909305282014	34.576233228162856	19.15872399006699
27	16.620141354393777	29.72776434786618	35.78321268369155	17.868881614048494
28	14.72496492487666	25.51980344757175	43.531752043578784	16.223479583972807
29	15.343736208724978	22.2907842995185	42.11662725518222	20.248852236574297
30	17.65480809132047	27.202355467437773	37.36379983796281	17.779036603278946
31	26.505463814987777	23.920641298405318	31.381729318850986	18.192165567755918
32	27.150187396668358	23.093856419241586	32.53930719221167	17.21664899187838
33	25.11510568645145	25.327861833654993	30.91854008444377	18.638492395449784
34	18.230632933070737	25.685134075894005	33.06941910326248	23.014813887772778
35	19.369240598878914	24.663245881554765	34.30432691991015	21.663186599656164
36	27.672526792124728	24.025372652601487	29.751213632201928	18.550886923071857
37	19.922406581608122	30.211636378007732	30.973079431157245	18.8928776092269
38	20.678316657554817	30.838707127660275	27.911498712265427	20.571477502519482
39	20.15176166042011	29.346384133529185	29.79863915108321	20.703215054967494
40	23.25615708385754	25.199812932675524	29.04602251394771	22.498007469519223
41	18.384502394330017	23.256420558962436	31.125894991996944	27.233182054710603
42	21.92402695350323	23.87255709176179	27.87856432415342	26.324851630581552
43	21.10461937727659	25.472246191138016	28.76384067660407	24.659293754981327
44	19.46646291258555	28.607599939400725	30.184498442203445	21.741438705810285
45	17.35523689705369	34.09143903515417	26.71703432421929	21.836289743572852
46	21.32923190420045	30.00889228479024	28.75277472219844	19.90910108881087
47	20.79016183958318	26.01540011988117	29.91535862255215	23.279079417983493
48	22.046147664622538	23.010993498751787	33.192198502144024	21.750660334481644
49	21.086966545248558	23.014286937562986	33.441050738718324	22.457695778470132
50	19.38465389251533	29.525678942410927	30.038138021433703	21.051529143640042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	259.0
1	522.0
2	785.0
3	9332.0
4	17879.0
5	13122.0
6	8365.0
7	8770.0
8	9175.0
9	9788.0
10	10401.0
11	10287.5
12	10174.0
13	9683.0
14	9192.0
15	8441.0
16	7690.0
17	7308.5
18	6927.0
19	6360.0
20	5793.0
21	5641.0
22	5489.0
23	4986.5
24	4484.0
25	4561.5
26	4639.0
27	5709.5
28	6780.0
29	6863.0
30	6946.0
31	8941.5
32	10937.0
33	13448.0
34	15959.0
35	17499.0
36	19039.0
37	20410.5
38	21782.0
39	28656.5
40	35531.0
41	45587.5
42	55644.0
43	65482.0
44	75320.0
45	75922.0
46	76524.0
47	70318.0
48	64112.0
49	59276.5
50	54441.0
51	50699.0
52	46957.0
53	42151.0
54	37345.0
55	34345.0
56	31345.0
57	27838.5
58	24332.0
59	22242.5
60	20153.0
61	18653.0
62	17153.0
63	14858.5
64	12564.0
65	10589.0
66	8614.0
67	7017.0
68	5420.0
69	4641.5
70	3863.0
71	3362.5
72	2862.0
73	2430.5
74	1999.0
75	1530.0
76	1061.0
77	811.0
78	561.0
79	449.0
80	337.0
81	243.0
82	149.0
83	100.0
84	51.0
85	38.5
86	26.0
87	18.0
88	10.0
89	8.0
90	6.0
91	7.0
92	8.0
93	5.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	759085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.429314083041437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66016591834129	22.266267863858932
2	7.585407708010607	4.464666917739352
3	3.4698142532836975	3.063427604090995
4	2.1000940213832284	2.472173062368183
5	1.4916021117279792	2.1948413516485283
6	1.0398185298997058	1.8360687663470912
7	0.8304595196209588	1.7107897825323841
8	0.6884656758973281	1.6208858089100706
9	0.556776013092274	1.474698254687651
>10	5.6095214826228545	36.444359742340126
>50	0.8670826008042759	17.05206444275128
>100	0.09989223526836444	3.8249984965231403
>500	4.499650237313714E-4	0.1198415661884522
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.499650237313714E-4	1.454916340013839
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10987	1.4474004887463194	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	905	0.11922248496545183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025425347622466524	0.0
2	0.0	0.0	0.0	0.09748578881152967	0.0
3	0.0	0.0	0.0	0.14254003174875013	0.0
4	0.0	0.0	0.0	0.23554674377704737	0.0
5	0.0	0.0	0.0	0.4062786117496723	0.0
6	0.0	0.0	0.0	0.6318133015406707	0.0
7	0.0	0.0	0.0	0.7942457037090708	0.0
8	0.0	0.0	0.0	1.2924771270674562	0.0
9	0.0	0.0	0.0	1.5505509923131138	0.0
10	0.0	0.0	0.0	1.9236317408458867	0.0
11	0.0	0.0	0.0	2.159178484622934	0.0
12	0.0	0.0	0.0	2.3764137086097077	0.0
13	1.3173755244801308E-4	0.0	0.0	2.4623065928058123	0.0
14	1.3173755244801308E-4	0.0	0.0	2.4941870804982313	0.0
15	1.3173755244801308E-4	0.0	0.0	2.5316005453934673	0.0
16	2.6347510489602616E-4	0.0	0.0	2.623685094554628	0.0
17	2.6347510489602616E-4	0.0	0.0	2.724727797282254	0.0
18	2.6347510489602616E-4	0.0	0.0	2.901651330219936	0.0
19	2.6347510489602616E-4	0.0	0.0	2.9662027309194623	0.0
20	2.6347510489602616E-4	0.0	0.0	3.046826113017646	0.0
21	2.6347510489602616E-4	0.0	0.0	3.1399645625983914	0.0
22	2.6347510489602616E-4	0.0	0.0	3.2327077995217928	0.0
23	2.6347510489602616E-4	0.0	0.0	3.3458703570746358	0.0
24	2.6347510489602616E-4	0.0	0.0	3.435188417634389	0.0
25	2.6347510489602616E-4	0.0	0.0	3.500135030991259	0.0
26	2.6347510489602616E-4	0.0	0.0	3.57100983420829	0.0
27	2.6347510489602616E-4	0.0	0.0	3.638459461061673	0.0
28	2.6347510489602616E-4	0.0	0.0	3.709729476936048	0.0
29	2.6347510489602616E-4	0.0	0.0	3.789035483509752	0.0
30	2.6347510489602616E-4	0.0	0.0	3.908126230922756	0.0
31	2.6347510489602616E-4	0.0	0.0	4.002055105818189	0.0
32	2.6347510489602616E-4	0.0	0.0	4.0891336279863255	0.0
33	2.6347510489602616E-4	0.0	0.0	4.17370913665795	0.0
34	2.6347510489602616E-4	0.0	0.0	4.267111061343591	0.0
35	2.6347510489602616E-4	0.0	0.0	4.392788686378996	0.0
36	2.6347510489602616E-4	0.0	0.0	4.483819335120573	0.0
37	2.6347510489602616E-4	0.0	0.0	4.5737960834425655	0.0
38	2.6347510489602616E-4	0.0	0.0	4.677737012324048	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACG	25	4.4414068E-5	44.0	1
CGATTGA	25	4.4414068E-5	44.0	10
CTAAGCG	30	2.5265763E-6	44.0	1
CTACGCT	30	2.5265763E-6	44.0	24
GGACGTA	20	7.8547915E-4	44.0	8
AACGACT	20	7.8547915E-4	44.0	41
CGGTCTA	35	1.4452235E-7	44.0	31
CGGTAGT	30	2.5265763E-6	44.0	12
CCACGTC	20	7.8547915E-4	44.0	14
TCGACCA	25	4.4414068E-5	44.0	29
TATCGTT	20	7.8547915E-4	44.0	13
AATACGG	30	2.5265763E-6	44.0	2
TACGTAG	30	2.5265763E-6	44.0	1
TAGTACG	20	7.8547915E-4	44.0	1
TATCCGG	20	7.8547915E-4	44.0	2
CGTTTTT	8840	0.0	43.552032	1
TCGTTGA	75	0.0	41.066666	24
CGTTGAT	75	0.0	41.066666	25
TGTTGCG	55	7.8216544E-11	40.0	1
ACCCGCT	215	0.0	39.90698	34
>>END_MODULE