FastQCFastQC Report
Thu 26 May 2016
SRR1546007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546007_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences748275
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108631.4517389996993084No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9700.12963148575055963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG551.8189894E-1244.0000041
GATACGT302.5265272E-644.09
ACCGGTG254.4413464E-544.016
GATCGTC207.8547193E-444.09
CGCGGTA207.8547193E-444.030
CGCATCG207.8547193E-444.021
CGTTGAT600.044.025
GAATCGG207.8547193E-444.02
ATTAACG454.783942E-1044.010
ACGGGTA302.5265272E-644.05
TCTACGG254.4413464E-544.02
AACATCG254.4413464E-544.033
GTCACGA207.8547193E-444.026
CGTACTC254.4413464E-544.030
TCGTACT254.4413464E-544.029
ACGTCCA207.8547193E-444.022
CGCACTG254.4413464E-544.041
TCGACGG408.305506E-944.02
TACGTAG207.8547193E-444.01
TGCGTAG207.8547193E-444.01
Produced by FastQC (version 0.11.5)