##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546007_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	748275
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.013417526978717	31.0	28.0	33.0	23.0	33.0
2	28.673645384384084	31.0	28.0	33.0	16.0	33.0
3	28.084161571614715	31.0	26.0	33.0	16.0	34.0
4	32.08055995456216	35.0	32.0	35.0	25.0	37.0
5	33.64915706124086	35.0	33.0	35.0	28.0	37.0
6	33.50451237847048	35.0	33.0	37.0	28.0	37.0
7	34.43830276302162	35.0	35.0	37.0	32.0	37.0
8	34.92088871070128	35.0	35.0	37.0	32.0	37.0
9	36.82050182085463	39.0	37.0	39.0	32.0	39.0
10	36.45673048010424	39.0	35.0	39.0	32.0	39.0
11	36.47205773278541	39.0	35.0	39.0	32.0	39.0
12	35.25108683304935	37.0	34.0	39.0	30.0	39.0
13	35.175839096588824	37.0	34.0	39.0	27.0	39.0
14	35.93346697404029	38.0	34.0	40.0	28.0	41.0
15	36.52092078447095	38.0	35.0	40.0	31.0	41.0
16	36.150098560021384	37.0	34.0	40.0	31.0	41.0
17	35.722485717149446	37.0	34.0	40.0	30.0	41.0
18	35.40946176205272	37.0	34.0	39.0	29.0	40.0
19	34.818164444889916	36.0	33.0	39.0	27.0	40.0
20	35.35935585179246	35.0	34.0	39.0	30.0	40.0
21	35.45796665664361	36.0	34.0	39.0	30.0	40.0
22	35.80708696669005	36.0	34.0	40.0	31.0	41.0
23	36.159039123316965	36.0	35.0	40.0	32.0	41.0
24	35.76483512077779	35.0	34.0	40.0	31.0	41.0
25	35.42247569409642	35.0	34.0	40.0	30.0	41.0
26	35.57225618923524	35.0	34.0	40.0	31.0	41.0
27	35.898609468444086	36.0	35.0	40.0	31.0	41.0
28	35.87009588720724	36.0	35.0	40.0	31.0	41.0
29	36.01329324112125	36.0	35.0	40.0	31.0	41.0
30	35.69259830944506	36.0	35.0	40.0	31.0	41.0
31	34.98718652901674	35.0	34.0	40.0	29.0	41.0
32	34.9375804350005	36.0	34.0	40.0	29.0	41.0
33	34.66313320637466	36.0	34.0	40.0	26.0	41.0
34	34.24041562259865	36.0	34.0	40.0	23.0	41.0
35	33.96203401155992	36.0	34.0	40.0	21.0	41.0
36	33.56230129297384	35.0	33.0	40.0	18.0	41.0
37	33.4520998296081	35.0	33.0	40.0	18.0	41.0
38	33.303032975844445	35.0	33.0	40.0	18.0	41.0
39	33.06253449600749	35.0	33.0	40.0	17.0	41.0
40	32.934061675186264	35.0	33.0	40.0	16.0	40.0
41	32.65820854632321	35.0	32.0	39.0	15.0	40.0
42	32.828432060405596	35.0	33.0	40.0	15.0	40.0
43	32.61536333567204	35.0	32.0	39.0	15.0	40.0
44	32.349473121512816	35.0	32.0	39.0	12.0	40.0
45	32.32773245130467	35.0	32.0	39.0	12.0	40.0
46	32.183249473789715	35.0	31.0	39.0	12.0	40.0
47	32.18949851324714	35.0	32.0	39.0	13.0	40.0
48	32.24227857405366	35.0	32.0	39.0	12.0	40.0
49	32.27755437506264	35.0	32.0	39.0	12.0	40.0
50	32.37867094316929	35.0	32.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	9.0
14	16.0
15	73.0
16	157.0
17	359.0
18	720.0
19	1259.0
20	1981.0
21	3158.0
22	4510.0
23	6333.0
24	9270.0
25	14191.0
26	20650.0
27	23540.0
28	22460.0
29	21614.0
30	23255.0
31	28004.0
32	38875.0
33	60899.0
34	100687.0
35	67973.0
36	66956.0
37	97158.0
38	109881.0
39	24280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.57659283017607	22.63392469346163	28.224382746984734	22.565099729377568
2	37.562527145768605	20.455447529317432	29.343356386355286	12.638668938558684
3	27.392202064748922	19.167418395643313	40.19284354014233	13.247535999465438
4	24.12909692292272	25.203835488289734	36.583742607998396	14.08332498078915
5	20.383816110387222	29.29885403093782	37.42033343356386	12.896996425111087
6	18.29367545354315	36.77124051986235	34.79990645150513	10.135177575089372
7	77.56600180414954	4.8043834151882665	15.600547926898534	2.0290668537636565
8	79.6874143864221	2.8596438475159536	15.163676455848451	2.2892653102134912
9	76.4522401523504	4.255521031706258	16.365373692826836	2.9268651231165013
10	46.066352611005314	22.877818983662422	21.197019812234807	9.858808593097459
11	41.21653135545087	19.239450736694398	28.452507433764325	11.091510474090407
12	36.81534195315893	20.03260833249808	30.65397079950553	12.49807891483746
13	19.598543316294144	35.88346530353145	31.10487454478634	13.413116835388058
14	14.661053756974374	36.88804249774481	34.28592429253951	14.164979452741305
15	13.632287594801376	21.165480605392403	51.19842303965788	14.003808760148342
16	15.535197621195415	17.318365574153887	49.51668838328155	17.629748421369147
17	15.824262470348469	17.771942133573887	35.29197153452942	31.111823861548228
18	20.212087801944474	21.095452874945707	40.051718953593266	18.640740369516553
19	25.97841702582607	22.40513180314724	32.561825532057064	19.05462563896963
20	27.373358725067654	22.15148174133841	32.23066386021182	18.244495673382115
21	20.834853496374997	25.377702048043833	34.90895726838395	18.87848718719722
22	20.698673615983427	22.107781230162708	33.163075072667134	24.03047008118673
23	17.909725702448966	27.166349269987638	32.227857405365675	22.69606762219772
24	17.808559687280745	22.23487354248104	43.91607363602954	16.04049313420868
25	16.23320303364405	22.4581871638101	41.404831111556575	19.903778690989277
26	15.60709632153954	30.666666666666664	34.705021549563995	19.021215462229797
27	16.582138919514886	29.675854465270124	35.776419097257026	17.96558751795797
28	14.679362533827803	25.494103103805422	43.598142394173266	16.228391968193513
29	15.355584511042064	22.209749089572682	42.20106244362032	20.233603955764927
30	17.651531856603523	27.21165346964686	37.38612141258228	17.750693261167353
31	26.606394707828002	23.830543583575555	31.466239016404394	18.096822692192042
32	27.066653302595974	23.071197086632587	32.60151682202399	17.26063278874745
33	25.141826200260596	25.29805218669607	30.90267615515686	18.657445457886475
34	18.25906251044068	25.65808025124453	33.10253583241455	22.980321405900238
35	19.314957736126424	24.650295680064147	34.37559720690923	21.659149376900203
36	27.596271424275837	23.98757141425278	29.84825097724767	18.567906184223716
37	19.85459891082824	30.22297951956166	31.113561190738697	18.808860378871405
38	20.709765794661052	30.71090174067021	28.03314289532592	20.546189569342822
39	20.129230563629683	29.390264274498012	29.86843072399853	20.612074437873776
40	23.28582406200929	25.201964518392302	29.114028933213053	22.398182486385355
41	18.48411346096021	23.275934649694296	31.05783301593665	27.18211887340884
42	21.942200394240086	23.854598910828237	27.814640339447394	26.38856035548428
43	21.075273128194848	25.44131502455648	28.833650730012362	24.64976111723631
44	19.432160636129765	28.545655006514988	30.300892051718954	21.721292305636297
45	17.263973806421436	34.17727439778157	26.862717583776018	21.696034212020983
46	21.26945307540677	30.000735023888275	28.938825966389363	19.790985934315593
47	20.71484414152551	26.130767431759715	29.962781063111827	23.191607363602955
48	22.038822625371687	23.108349203167286	33.19848986001136	21.654338311449667
49	21.035314556814004	23.042330693929372	33.680398249306734	22.241956499949886
50	19.561658481173367	29.51227823995189	30.085864154221376	20.84019912465337
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	288.0
1	509.0
2	730.0
3	9323.0
4	17916.0
5	13207.0
6	8498.0
7	8784.0
8	9070.0
9	9709.5
10	10349.0
11	10186.0
12	10023.0
13	9548.5
14	9074.0
15	8347.5
16	7621.0
17	7258.0
18	6895.0
19	6249.5
20	5604.0
21	5459.0
22	5314.0
23	4917.5
24	4521.0
25	4490.5
26	4460.0
27	5579.5
28	6699.0
29	6796.5
30	6894.0
31	8804.0
32	10714.0
33	13273.0
34	15832.0
35	17441.0
36	19050.0
37	20270.0
38	21490.0
39	28140.0
40	34790.0
41	44998.0
42	55206.0
43	64773.0
44	74340.0
45	74901.0
46	75462.0
47	69551.5
48	63641.0
49	58736.5
50	53832.0
51	49900.0
52	45968.0
53	41193.5
54	36419.0
55	33469.5
56	30520.0
57	27454.0
58	24388.0
59	21909.0
60	19430.0
61	18236.5
62	17043.0
63	14609.5
64	12176.0
65	10187.5
66	8199.0
67	6682.5
68	5166.0
69	4447.0
70	3728.0
71	3254.0
72	2780.0
73	2373.0
74	1966.0
75	1496.5
76	1027.0
77	809.5
78	592.0
79	451.0
80	310.0
81	222.0
82	134.0
83	96.5
84	59.0
85	40.5
86	22.0
87	19.5
88	17.0
89	11.0
90	5.0
91	5.0
92	5.0
93	5.5
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	748275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.190959192844495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6555136279664	22.084570110276577
2	7.537303249839806	4.40042223180336
3	3.3479006174531665	2.931852909173228
4	2.086914446904682	2.4367613783420885
5	1.5115975871332268	2.2062491741004115
6	1.0920015013584428	1.9125942758807537
7	0.825696776678699	1.68720166395838
8	0.7160499917901969	1.6721748872307418
9	0.562348937597445	1.4773954400591822
>10	5.689332488404367	36.69710716682235
>50	0.8815756862459728	17.322846137739255
>100	0.09284582305286827	3.583176585405258
>500	4.5963278739043694E-4	0.1301460828219539
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.5963278739043694E-4	1.4575019563864793
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10863	1.4517389996993084	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	970	0.12963148575055963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024188967959640508	0.0
2	0.0	0.0	0.0	0.09421669840633456	0.0
3	0.0	0.0	0.0	0.13644716180548594	0.0
4	0.0	0.0	0.0	0.23320303364404799	0.0
5	0.0	0.0	0.0	0.39958571380842606	0.0
6	0.0	0.0	0.0	0.6198255988774181	0.0
7	0.0	0.0	0.0	0.7785907587451137	0.0
8	0.0	0.0	0.0	1.2373793057365274	0.0
9	0.0	0.0	0.0	1.4927666967358257	0.0
10	0.0	0.0	0.0	1.8513247135077344	0.0
11	0.0	0.0	0.0	2.0822558551334738	0.0
12	0.0	0.0	0.0	2.286859777488223	0.0
13	0.0	0.0	0.0	2.3723898299422004	0.0
14	0.0	0.0	0.0	2.4023253483010927	0.0
15	0.0	0.0	0.0	2.4456249373559187	0.0
16	0.0	0.0	0.0	2.5336941632421235	0.0
17	0.0	0.0	0.0	2.6348601784103436	0.0
18	0.0	0.0	0.0	2.8029801877651934	0.0
19	0.0	0.0	0.0	2.871404229728375	0.0
20	0.0	0.0	0.0	2.9630817547024826	0.0
21	0.0	0.0	0.0	3.058902141592329	0.0
22	1.336407069593398E-4	0.0	0.0	3.1549898098960942	0.0
23	2.672814139186796E-4	0.0	0.0	3.2540175737529653	0.0
24	2.672814139186796E-4	0.0	0.0	3.3312619023754637	0.0
25	2.672814139186796E-4	0.0	0.0	3.397414052320337	0.0
26	2.672814139186796E-4	0.0	0.0	3.45955698105643	0.0
27	2.672814139186796E-4	0.0	0.0	3.528114663726571	0.0
28	2.672814139186796E-4	0.0	0.0	3.601617052554208	0.0
29	2.672814139186796E-4	0.0	0.0	3.677925896227991	0.0
30	2.672814139186796E-4	0.0	0.0	3.7887140422972836	0.0
31	2.672814139186796E-4	0.0	0.0	3.8790551602017973	0.0
32	2.672814139186796E-4	0.0	0.0	3.961377835688751	0.0
33	2.672814139186796E-4	0.0	0.0	4.041428619157395	0.0
34	2.672814139186796E-4	0.0	0.0	4.1277605158531285	0.0
35	2.672814139186796E-4	0.0	0.0	4.243359727372958	0.0
36	2.672814139186796E-4	0.0	0.0	4.335037252347065	0.0
37	2.672814139186796E-4	0.0	0.0	4.435668704687448	0.0
38	2.672814139186796E-4	0.0	0.0	4.542046707427082	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	55	1.8189894E-12	44.000004	1
GATACGT	30	2.5265272E-6	44.0	9
ACCGGTG	25	4.4413464E-5	44.0	16
GATCGTC	20	7.8547193E-4	44.0	9
CGCGGTA	20	7.8547193E-4	44.0	30
CGCATCG	20	7.8547193E-4	44.0	21
CGTTGAT	60	0.0	44.0	25
GAATCGG	20	7.8547193E-4	44.0	2
ATTAACG	45	4.783942E-10	44.0	10
ACGGGTA	30	2.5265272E-6	44.0	5
TCTACGG	25	4.4413464E-5	44.0	2
AACATCG	25	4.4413464E-5	44.0	33
GTCACGA	20	7.8547193E-4	44.0	26
CGTACTC	25	4.4413464E-5	44.0	30
TCGTACT	25	4.4413464E-5	44.0	29
ACGTCCA	20	7.8547193E-4	44.0	22
CGCACTG	25	4.4413464E-5	44.0	41
TCGACGG	40	8.305506E-9	44.0	2
TACGTAG	20	7.8547193E-4	44.0	1
TGCGTAG	20	7.8547193E-4	44.0	1
>>END_MODULE