FastQCFastQC Report
Thu 26 May 2016
SRR1546006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546006_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences663426
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56340.8492280977833248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGATC302.5260924E-644.022
CCGTCAC254.4407985E-544.036
GACGGTC254.4407985E-544.029
CGTACAG254.4407985E-544.013
TCGTAAG302.5260924E-644.01
ACGTCGG207.854073E-444.02
TTACGAC408.303687E-944.024
CGGTCTA408.303687E-944.031
GCGTGTA207.854073E-444.018
GCGGAAT3350.044.08
CGACTAT302.5260924E-644.028
TTACCGG454.783942E-1044.02
ACGACGT351.4448415E-744.026
CTTACGA408.303687E-944.023
TTCTCGG254.4407985E-544.02
CGTTTTT81550.043.379521
CATACGA1800.042.7777818
ATACGAA1650.042.66666819
ACGGGAT1850.041.6216245
TACGGGA1650.041.3333324
Produced by FastQC (version 0.11.5)