FastQCFastQC Report
Thu 26 May 2016
SRR1546005_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546005_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1007570
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168991.6772035689828002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG207.8560173E-444.0000041
ATTGTCG207.8560173E-444.0000041
TACGGGT207.8560173E-444.0000044
TATTACG207.8560173E-444.0000041
TACGTAG408.310963E-944.0000041
CTATGCG302.527404E-644.01
CTGTCGA254.4424476E-544.014
ATGTGCG351.4458419E-744.01
CCGCTCG302.527404E-644.019
CGTTTTT134100.043.278151
CGGTCTA1050.041.90476231
ACGGTCT1050.041.90476230
TTACGGG2950.041.016953
CGCATGG1250.040.482
TACGGGA3000.040.3333324
TATACGG1150.040.173912
TCACGAC1100.040.025
CTCACGA1100.040.024
CGACGGT1100.040.028
TATAGCG501.3478711E-939.6000021
Produced by FastQC (version 0.11.5)