FastQCFastQC Report
Thu 26 May 2016
SRR1546004_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546004_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1005189
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173021.7212683385910512No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10180.101274486688573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC207.8560103E-444.025
CATATCG302.5273985E-644.01
CGTATGA207.8560103E-444.015
CTCCGAC207.8560103E-444.025
CGTTCGA254.442441E-544.014
GTATGCG254.442441E-544.01
TGCGACG254.442441E-544.01
TATTACG207.8560103E-444.01
TTACGTA207.8560103E-444.038
TATAGCG254.442441E-544.01
TGTTACG351.4458419E-743.9999961
CGTTTTT136750.043.1795271
TACGGGA2800.042.428574
GCGTAAG900.041.5555571
TGCGTAG1450.040.965521
ACGGGCC650.040.6153835
CGTAAGG1550.039.7419362
GTTTTTT151700.039.6783142
CCGACGG501.3478711E-939.62
TATACGG1300.038.9230772
Produced by FastQC (version 0.11.5)