##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546004_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1005189
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.06265289413235	31.0	28.0	33.0	25.0	33.0
2	28.753658267251232	31.0	28.0	33.0	16.0	33.0
3	28.256313986722894	31.0	27.0	33.0	16.0	34.0
4	32.14209865010461	35.0	32.0	35.0	25.0	37.0
5	33.72637285127473	35.0	33.0	35.0	30.0	37.0
6	33.5698958106386	35.0	33.0	37.0	28.0	37.0
7	34.44628721563805	35.0	35.0	37.0	32.0	37.0
8	34.88330652245498	35.0	35.0	37.0	32.0	37.0
9	36.79801410481014	39.0	37.0	39.0	32.0	39.0
10	36.33848858274414	39.0	35.0	39.0	32.0	39.0
11	36.429061599360914	39.0	35.0	39.0	32.0	39.0
12	35.14130178503744	37.0	34.0	39.0	29.0	39.0
13	35.36567352010418	37.0	35.0	39.0	30.0	39.0
14	36.119355663462294	38.0	35.0	40.0	29.0	41.0
15	36.74441025518584	38.0	35.0	40.0	31.0	41.0
16	36.35499592613926	38.0	35.0	40.0	31.0	41.0
17	35.98670299814264	38.0	34.0	40.0	30.0	41.0
18	35.63047347314784	37.0	34.0	39.0	29.0	40.0
19	35.08523372221543	37.0	34.0	39.0	28.0	41.0
20	35.3905743099059	35.0	34.0	39.0	30.0	41.0
21	35.49776012272319	36.0	34.0	39.0	30.0	40.0
22	35.90593211823846	36.0	34.0	40.0	31.0	41.0
23	36.256387604719116	37.0	35.0	40.0	32.0	41.0
24	35.81129419442513	36.0	35.0	40.0	31.0	41.0
25	35.42786878885463	35.0	34.0	40.0	30.0	41.0
26	35.602444913344655	36.0	34.0	40.0	30.0	41.0
27	35.94556148147264	37.0	35.0	40.0	31.0	41.0
28	35.89086927930966	36.0	35.0	40.0	31.0	41.0
29	36.06414117146129	37.0	35.0	40.0	31.0	41.0
30	35.72804318391864	36.0	35.0	40.0	31.0	41.0
31	35.03278388442372	36.0	34.0	40.0	29.0	41.0
32	34.98069815726197	36.0	34.0	40.0	29.0	41.0
33	34.648340759797414	36.0	34.0	40.0	25.0	41.0
34	34.17433736342121	36.0	34.0	40.0	22.0	41.0
35	33.8809069737134	36.0	34.0	40.0	18.0	41.0
36	33.40432495779401	36.0	33.0	40.0	17.0	41.0
37	33.28228422714534	36.0	33.0	40.0	15.0	41.0
38	33.16279326574406	36.0	33.0	40.0	15.0	41.0
39	32.92962020077816	36.0	33.0	40.0	15.0	41.0
40	32.72994332409129	35.0	32.0	40.0	15.0	40.0
41	32.443046034128905	35.0	32.0	39.0	12.0	40.0
42	32.615163914447926	35.0	32.0	40.0	12.0	40.0
43	32.38700483192713	35.0	32.0	39.0	10.0	40.0
44	32.20628160475294	35.0	31.0	39.0	10.0	40.0
45	32.124445253579175	35.0	31.0	39.0	10.0	40.0
46	31.94539733323783	35.0	31.0	39.0	10.0	40.0
47	31.912717906781708	35.0	31.0	39.0	10.0	40.0
48	31.940518648731732	35.0	31.0	39.0	10.0	40.0
49	32.00254578989623	35.0	31.0	39.0	10.0	40.0
50	32.15358604202792	35.0	32.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	0.0
12	3.0
13	9.0
14	22.0
15	115.0
16	214.0
17	546.0
18	1085.0
19	1857.0
20	3037.0
21	4283.0
22	6407.0
23	9056.0
24	13513.0
25	20590.0
26	29705.0
27	32998.0
28	30809.0
29	28653.0
30	30857.0
31	36837.0
32	49593.0
33	75974.0
34	122030.0
35	90747.0
36	95935.0
37	137882.0
38	153013.0
39	29416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.76024110888599	22.107384780374638	27.76283863034713	23.369535480392244
2	38.14775131840878	20.314886056254096	29.519025775252217	12.01833685008491
3	27.14464642967641	18.312178107798633	41.64281543072994	12.900360031795016
4	23.897595377585706	25.054392755989173	37.20932083419138	13.838691032233738
5	19.5101617705725	29.068563225423276	38.75052353338526	12.670751470618956
6	17.786306853735965	36.66086676237006	35.93463517806104	9.618191205832934
7	76.49954386687479	4.368929624180129	17.015904471696366	2.1156220372487162
8	78.03726463381513	2.9512857780974526	16.657464417139465	2.353985170947951
9	74.50598842605719	4.77840485719601	17.816151987337705	2.899454729409096
10	41.9963807801319	25.713273822136934	22.575555442807275	9.7147899549239
11	36.62843505052284	19.3410393468293	32.31611169640734	11.714413906240518
12	33.816128111230825	19.96878199025258	32.784182875061305	13.43090702345529
13	19.561594884146167	33.08979704314313	33.572691304819294	13.775916767891411
14	14.851137447783453	34.347669940677825	36.36450458570478	14.436688025833947
15	14.183402325333844	21.51515784593743	49.953889268585314	14.347550560143416
16	16.30270526239344	18.440412698507444	47.83886413400863	17.418017905090487
17	16.91960417394142	18.483588658451296	36.81238055728823	27.784426610319052
18	20.046876756510468	21.05932317206018	40.49298191683355	18.400818154595804
19	24.64640977965338	22.44433633873829	34.46874169932222	18.440512182286117
20	26.187711962625933	21.647073336457122	34.02802855980318	18.137186141113762
21	20.41805073473745	24.84587475589168	36.1978692564284	18.53820525294248
22	20.52131489699947	21.922145984486498	34.38268823077053	23.173850887743498
23	17.630316288777532	25.702629057818978	34.80459893612047	21.86245571728302
24	18.030241079040856	21.19153711391589	44.41085208851271	16.367369718530544
25	16.62722134842303	23.141518659674947	40.9477222691454	19.283537722756616
26	15.933421475961238	30.11105374213208	35.43622144691197	18.519303334994714
27	16.237543387362972	29.08676875692034	37.05372820434764	17.621959651369046
28	14.804479555586065	24.84836185035849	43.33672573018607	17.010432863869383
29	15.461370946160374	22.04272032423753	42.675854988464856	19.82005374113724
30	18.171707012313107	26.46069545130319	38.0291666542312	17.33843088215251
31	25.118659277011584	23.182903911602693	34.08861418101471	17.609822630371006
32	25.70143525247491	23.487224790561775	34.040961451030604	16.770378505932715
33	24.828564578402666	24.23972009244033	32.655251897901785	18.276463431255216
34	19.042687494590567	25.375028974650537	33.497780019478924	22.08450351127997
35	19.443607122640618	24.76499444383096	35.34489533809065	20.446503095437773
36	26.66304545712299	24.32000350182901	30.314796520853292	18.70215452019471
37	19.785632353716565	29.62228993751424	31.413793823848053	19.178283884921143
38	20.668550889434723	29.5216123534977	29.646862430846337	20.16297432622124
39	20.672729208138968	28.526774566773017	30.44492130335688	20.355574921731137
40	23.236426184528483	24.64004281781834	29.746246725740132	22.37728427191304
41	18.146239164972954	23.2475683677398	31.719407991929877	26.886784475357373
42	21.33708188211371	24.087211459735432	28.62058777006115	25.9551188880897
43	21.33300304718814	24.95550587998874	29.394372600575615	24.317118472247508
44	19.49812423335313	28.558907827284223	30.746954055406494	21.19601388395615
45	18.017706122928125	32.84705662318231	27.33983360343179	21.795403650457775
46	21.065192715001853	29.406410137794982	28.881832172855056	20.64656497434811
47	20.832301189129605	25.840215123723002	30.75222669567614	22.575256991471257
48	22.089875635328283	23.723498764908886	32.65286428721365	21.53376131254918
49	20.42899395039142	23.92803741385948	33.56413569985346	22.078832935895637
50	19.61869857310416	29.54409568747768	29.773007862203027	21.06419787721513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	285.0
1	629.5
2	974.0
3	13794.0
4	26614.0
5	19413.0
6	12212.0
7	12494.5
8	12777.0
9	13538.0
10	14299.0
11	14295.0
12	14291.0
13	13888.0
14	13485.0
15	12633.0
16	11781.0
17	11016.5
18	10252.0
19	9607.0
20	8962.0
21	8517.5
22	8073.0
23	7773.5
24	7474.0
25	7628.0
26	7782.0
27	8154.5
28	8527.0
29	10289.5
30	12052.0
31	14734.0
32	17416.0
33	19665.5
34	21915.0
35	24164.5
36	26414.0
37	29553.5
38	32693.0
39	39958.0
40	47223.0
41	59254.5
42	71286.0
43	81089.5
44	90893.0
45	89802.0
46	88711.0
47	84792.0
48	80873.0
49	78668.0
50	76463.0
51	72391.0
52	68319.0
53	60335.5
54	52352.0
55	46089.0
56	39826.0
57	35538.5
58	31251.0
59	28410.0
60	25569.0
61	22197.5
62	18826.0
63	16581.0
64	14336.0
65	12260.0
66	10184.0
67	8697.5
68	7211.0
69	6052.5
70	4894.0
71	4411.5
72	3929.0
73	3081.5
74	2234.0
75	1669.0
76	1104.0
77	854.5
78	605.0
79	489.0
80	373.0
81	313.5
82	254.0
83	165.5
84	77.0
85	69.0
86	61.0
87	47.5
88	34.0
89	25.0
90	16.0
91	10.5
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1005189.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.8373339811139
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41565146823369	22.800393142420855
2	7.178276549024395	4.283612696040773
3	3.281080580845134	2.936960913288704
4	2.105416356301116	2.5128004396902526
5	1.4550168429908958	2.170691174623266
6	1.1261322077731497	2.0160469674129975
7	0.8958291280779015	1.8710407019129283
8	0.7195357293276625	1.717522229383507
9	0.5824868823723394	1.5641870084065166
>10	5.257913814717224	33.63752145689961
>50	0.8411788296253836	17.1579327440113
>100	0.14081108175037296	5.498667962398086
>500	0.0	0.0
>1k	3.3526448035782865E-4	0.10183459441345075
>5k	0.0	0.0
>10k+	3.3526448035782865E-4	1.7307879690977652
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17302	1.7212683385910512	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1018	0.101274486688573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02765649047094626	0.0
2	0.0	0.0	0.0	0.10406003249140211	0.0
3	0.0	0.0	0.0	0.14265973861631992	0.0
4	0.0	0.0	0.0	0.21816792662872356	0.0
5	0.0	0.0	0.0	0.36411062994123494	0.0
6	0.0	0.0	0.0	0.5589993523606008	0.0
7	0.0	0.0	0.0	0.6979781911660394	0.0
8	0.0	0.0	0.0	1.1103384537634216	0.0
9	0.0	0.0	0.0	1.3235321914585216	0.0
10	0.0	0.0	0.0	1.6525250475283753	0.0
11	0.0	0.0	0.0	1.8705934903784263	0.0
12	0.0	0.0	0.0	2.068566209936639	0.0
13	0.0	0.0	0.0	2.153724324480272	0.0
14	0.0	0.0	0.0	2.185658617434134	0.0
15	9.948377867246855E-5	0.0	0.0	2.225551612681794	0.0
16	1.989675573449371E-4	0.0	0.0	2.3103117921107374	0.0
17	1.989675573449371E-4	0.0	0.0	2.4209377539945223	0.0
18	1.989675573449371E-4	0.0	0.0	2.5796143809771097	0.0
19	1.989675573449371E-4	0.0	0.0	2.6586045012430497	0.0
20	1.989675573449371E-4	0.0	0.0	2.7537109936539297	0.0
21	1.989675573449371E-4	0.0	0.0	2.859959669276126	0.0
22	1.989675573449371E-4	0.0	0.0	2.972873758069378	0.0
23	1.989675573449371E-4	0.0	0.0	3.097526932745981	0.0
24	1.989675573449371E-4	0.0	0.0	3.1899473631327044	0.0
25	1.989675573449371E-4	0.0	0.0	3.273314769660233	0.0
26	1.989675573449371E-4	0.0	0.0	3.3501162467953787	0.0
27	1.989675573449371E-4	0.0	0.0	3.4321903642001654	0.0
28	1.989675573449371E-4	0.0	0.0	3.518940219202558	0.0
29	1.989675573449371E-4	0.0	0.0	3.6186229654323716	0.0
30	2.9845133601740566E-4	0.0	0.0	3.754915742213653	0.0
31	2.9845133601740566E-4	0.0	0.0	3.865939639212128	0.0
32	2.9845133601740566E-4	0.0	0.0	3.963334258532475	0.0
33	3.979351146898742E-4	0.0	0.0	4.06510616411441	0.0
34	4.974188933623428E-4	0.0	0.0	4.171454323515279	0.0
35	4.974188933623428E-4	0.0	0.0	4.302176008690903	0.0
36	4.974188933623428E-4	0.0	0.0	4.409817457214514	0.0
37	4.974188933623428E-4	0.0	0.0	4.526312962039975	0.0
38	4.974188933623428E-4	0.0	0.0	4.664197479280016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	20	7.8560103E-4	44.0	25
CATATCG	30	2.5273985E-6	44.0	1
CGTATGA	20	7.8560103E-4	44.0	15
CTCCGAC	20	7.8560103E-4	44.0	25
CGTTCGA	25	4.442441E-5	44.0	14
GTATGCG	25	4.442441E-5	44.0	1
TGCGACG	25	4.442441E-5	44.0	1
TATTACG	20	7.8560103E-4	44.0	1
TTACGTA	20	7.8560103E-4	44.0	38
TATAGCG	25	4.442441E-5	44.0	1
TGTTACG	35	1.4458419E-7	43.999996	1
CGTTTTT	13675	0.0	43.179527	1
TACGGGA	280	0.0	42.42857	4
GCGTAAG	90	0.0	41.555557	1
TGCGTAG	145	0.0	40.96552	1
ACGGGCC	65	0.0	40.615383	5
CGTAAGG	155	0.0	39.741936	2
GTTTTTT	15170	0.0	39.678314	2
CCGACGG	50	1.3478711E-9	39.6	2
TATACGG	130	0.0	38.923077	2
>>END_MODULE