FastQCFastQC Report
Thu 26 May 2016
SRR1546003_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546003_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences989669
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172611.744118488100567No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9920.10023553329446512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG207.85595E-444.0000044
CGATATC207.85595E-444.0000049
GCGTATG408.310963E-944.0000041
TCGTAAG302.5273584E-644.01
CGCACAT302.5273584E-644.041
TTCGAAT254.4423898E-544.016
CGTTTTT128700.043.1794851
TACGGGA2050.041.8536574
TATACGG1200.040.3333322
CTCACGA557.8216544E-1140.024
TCGATTG557.8216544E-1140.01
TACGTAG557.8216544E-1140.01
CTCGTAG501.3478711E-939.6000021
GTTTTTT144500.039.4477542
GTACTAG950.039.3684231
GTAGGGT3250.039.261544
CGTATGG900.039.111112
TATGGGC1700.038.8235324
GACGTCG404.123758E-738.50000419
CTAACGG404.123758E-738.5000042
Produced by FastQC (version 0.11.5)