FastQCFastQC Report
Thu 26 May 2016
SRR1546002_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546002_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences857321
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84530.9859784141529252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG408.309144E-944.0000041
CTATCGA207.8553596E-444.00000438
TTGCTCG207.8553596E-444.0000041
CGTAAGA207.8553596E-444.00000426
TATAGCG650.044.0000041
TCGAACG207.8553596E-444.0000041
CGAACGG551.8189894E-1244.02
ACGTTAG302.52696E-644.01
TCGACAG351.4455145E-744.01
ACGACGT351.4455145E-744.026
CGTTTTT115550.043.0289961
TATACGG1150.042.086962
TACGGGA2150.040.9302334
CGCATGG1400.040.8571432
CGTTAGG1350.040.740742
GCGGAAT5350.040.299078
CGTAGGC1150.040.1739163
ATACGAA2300.040.17391619
ATGGGAC3400.040.1176455
TCTAGCG557.8216544E-1140.01
Produced by FastQC (version 0.11.5)