##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546002_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	857321
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.998567631027353	28.0	25.0	30.0	16.0	33.0
2	26.07564494512557	30.0	25.0	31.0	10.0	33.0
3	26.415020744855195	30.0	25.0	31.0	16.0	33.0
4	28.03488541631431	32.0	19.0	35.0	10.0	37.0
5	31.28958231514217	33.0	30.0	35.0	19.0	37.0
6	29.796488129883674	33.0	28.0	35.0	17.0	37.0
7	29.890386448016553	32.0	28.0	35.0	17.0	35.0
8	32.529101701696334	33.0	32.0	35.0	28.0	35.0
9	27.149661562005363	31.0	23.0	34.0	10.0	35.0
10	31.869128366154566	34.0	31.0	37.0	18.0	37.0
11	33.89434062620652	35.0	32.0	37.0	27.0	39.0
12	33.2738227571703	35.0	31.0	39.0	25.0	39.0
13	33.34586695065209	35.0	32.0	39.0	24.0	39.0
14	33.0938598261328	35.0	31.0	39.0	22.0	40.0
15	32.058428523271914	34.0	27.0	38.0	18.0	40.0
16	30.623163319223487	34.0	27.0	38.0	17.0	40.0
17	30.840122894458435	33.0	27.0	38.0	17.0	40.0
18	31.71880194232965	34.0	29.0	38.0	18.0	39.0
19	31.38600010964388	34.0	28.0	37.0	18.0	39.0
20	32.13626284670503	34.0	30.0	37.0	23.0	39.0
21	32.894836356510574	35.0	31.0	37.0	24.0	39.0
22	33.23984598534271	35.0	31.0	37.0	25.0	39.0
23	34.49691305823606	35.0	33.0	38.0	29.0	40.0
24	34.11977660642863	35.0	33.0	38.0	27.0	40.0
25	32.824154546546744	35.0	31.0	38.0	23.0	39.0
26	26.476800404982498	33.0	20.0	36.0	0.0	39.0
27	29.77060167661821	34.0	24.0	37.0	15.0	39.0
28	31.319704054840603	34.0	28.0	37.0	20.0	39.0
29	32.67957042927912	35.0	30.0	37.0	23.0	39.0
30	32.413363256003294	35.0	31.0	38.0	23.0	39.0
31	31.298547451887917	34.0	29.0	36.0	20.0	39.0
32	31.58082445198473	34.0	30.0	37.0	19.0	39.0
33	31.35033902120676	34.0	30.0	37.0	18.0	39.0
34	31.280351233668604	34.0	30.0	37.0	16.0	39.0
35	30.467196067750585	34.0	29.0	37.0	11.0	39.0
36	30.162463068092347	34.0	27.0	37.0	10.0	39.0
37	30.166799833434617	34.0	27.0	37.0	10.0	39.0
38	29.900417696522073	34.0	27.0	37.0	10.0	39.0
39	29.76396472266514	34.0	27.0	37.0	10.0	39.0
40	29.695264667493273	33.0	27.0	37.0	10.0	38.0
41	29.12378444013386	33.0	25.0	36.0	10.0	38.0
42	29.32294554781698	33.0	26.0	36.0	9.0	38.0
43	28.843717813980994	33.0	25.0	36.0	9.0	38.0
44	28.591838996128637	33.0	24.0	36.0	8.0	38.0
45	28.059982200366022	32.0	23.0	35.0	8.0	38.0
46	27.65687297989901	31.0	23.0	35.0	8.0	38.0
47	27.750001458030304	31.0	23.0	35.0	8.0	38.0
48	27.827827616493707	32.0	23.0	35.0	8.0	38.0
49	27.677912940427216	31.0	23.0	35.0	8.0	38.0
50	29.536592478196614	34.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	5.0
12	31.0
13	100.0
14	250.0
15	555.0
16	1031.0
17	2105.0
18	3658.0
19	5819.0
20	8713.0
21	12220.0
22	16448.0
23	22058.0
24	28458.0
25	35628.0
26	41929.0
27	46167.0
28	50762.0
29	59583.0
30	71465.0
31	83977.0
32	92110.0
33	92999.0
34	81630.0
35	62142.0
36	31007.0
37	6279.0
38	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.939783348360766	23.106747647613904	27.563654687100865	24.389814316924465
2	37.74852126566362	17.645549333330223	31.490771834587044	13.115157566419114
3	28.492361670832743	14.12551424728894	43.33989252567008	14.042231556208234
4	25.192197554941497	24.816608948106953	35.99923482569539	13.991958671256159
5	18.71772649917592	29.555090800295336	39.38186513569597	12.345317564832776
6	17.667594751557466	35.22904489683561	37.139764452288	9.963595899318925
7	78.91933126565196	3.030486830487064	16.71544264050455	1.3347392633564323
8	81.02857622757404	1.6562057852309697	16.02316985119926	1.2920481359957354
9	68.95328587541889	3.080176503316727	16.031334820913052	11.935202800351327
10	43.07861349482866	25.9900317384037	22.49204207058966	8.439312696177979
11	37.75412010203879	18.909836572299056	32.42472772742065	10.911315598241499
12	34.502712519581344	20.219964284089624	33.066844274198345	12.210478922130683
13	19.109994972711505	34.15593459159405	33.47602589928393	13.258044536410516
14	14.198182477741708	34.74742832614622	36.87918527599347	14.175203920118602
15	12.778527529361813	19.878435265204047	50.271601885408145	17.071435320025987
16	14.62812645438523	16.6243449069835	47.82677666824912	20.920751970382156
17	16.08242420283651	17.558534084666068	37.55594462284255	28.803097089654866
18	19.564317216071927	20.696565230526257	41.49519258247494	18.243924970926876
19	25.404486767500156	21.614657753630205	35.11100276325904	17.869852715610605
20	26.63599748518933	21.51924425040329	34.42969436185513	17.41506390255225
21	20.14554641726961	24.635929832583127	36.836144221359326	18.382379528787933
22	20.0501329140427	21.652566541587106	34.481366955901	23.815933588469196
23	16.925165719724582	25.760829374295042	35.45626433972806	21.857740566252314
24	17.129523247418412	20.636027812219695	46.50871727159372	15.725731668768175
25	15.973946748067528	22.11692003345305	42.54520768766891	19.363925530810512
26	12.559706341032124	42.072339298815734	29.37172890900841	15.996225451143737
27	15.44637306213192	30.080564922590252	37.56119353194428	16.91186848333355
28	14.245072732383788	24.78674848743936	44.56522119486167	16.402957585315185
29	14.605147896762121	21.957003269487156	43.79934703570775	19.638501798042974
30	17.74924444869541	26.547815812280344	38.55708655217824	17.145853186846
31	25.800021228921256	22.878011853203176	34.49408098016962	16.827885937705947
32	26.23369776314823	22.643210652719343	34.481133671052035	16.6419579130804
33	25.13096028208804	23.814650521799884	33.00607357104282	18.048315625069257
34	18.695097868826263	24.802378572320052	33.56257457825016	22.939948980603532
35	19.040476087719767	24.455367359483787	35.520301030769104	20.98385552202734
36	26.77036955819349	23.865389976449894	30.27454127450512	19.0896991908515
37	19.125625057592195	30.43725745665859	31.011838039660756	19.425279446088457
38	19.957985398701304	30.468750911268945	29.444047212187733	20.12921647784202
39	20.240260065949627	29.080239490225946	30.174578716723378	20.50492172710105
40	23.25068439942565	24.166094146766497	29.686546812687432	22.89667464112042
41	17.485282642090887	22.806976616693163	31.69921184713777	28.008528894078182
42	20.624363569771415	23.64236966083882	27.879755657449195	27.85351111194057
43	20.873628430891113	24.320178789508248	29.37581139386531	25.43038138573533
44	18.632577529303493	28.469266470785154	30.918290815225568	21.97986518468578
45	17.3314312841981	34.115809597571975	26.64194624883795	21.910812869391975
46	20.22323027197514	29.448829551591526	28.31844781592892	22.00949236050441
47	20.11825208994064	25.507248743469486	30.11812378327371	24.25637538331617
48	21.411466650181204	23.29500852072911	33.24670689275079	22.046817936338897
49	20.001259738184416	23.96395282513784	33.31879191108115	22.715995525596597
50	19.189428463784278	30.310467141245812	29.429116981853937	21.070987413115976
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	175.0
1	372.5
2	570.0
3	7679.5
4	14789.0
5	12270.0
6	9751.0
7	9809.0
8	9867.0
9	10834.5
10	11802.0
11	12305.5
12	12809.0
13	12836.5
14	12864.0
15	12532.5
16	12201.0
17	11665.0
18	11129.0
19	10647.5
20	10166.0
21	9639.5
22	9113.0
23	8602.0
24	8091.0
25	7954.0
26	7817.0
27	8031.5
28	8246.0
29	9675.0
30	11104.0
31	13362.0
32	15620.0
33	17519.5
34	19419.0
35	20954.0
36	22489.0
37	25118.5
38	27748.0
39	33767.0
40	39786.0
41	49977.5
42	60169.0
43	68352.5
44	76536.0
45	76329.0
46	76122.0
47	72082.0
48	68042.0
49	65409.0
50	62776.0
51	59226.5
52	55677.0
53	49782.0
54	43887.0
55	38916.0
56	33945.0
57	30430.0
58	26915.0
59	24375.5
60	21836.0
61	19062.5
62	16289.0
63	14249.0
64	12209.0
65	10554.5
66	8900.0
67	7547.0
68	6194.0
69	5329.5
70	4465.0
71	3944.5
72	3424.0
73	2692.0
74	1960.0
75	1506.5
76	1053.0
77	818.5
78	584.0
79	473.5
80	363.0
81	291.0
82	219.0
83	165.0
84	111.0
85	79.5
86	48.0
87	37.5
88	27.0
89	19.0
90	11.0
91	6.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	857321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.82543310381939
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.78479713278422	36.66038952785059
2	6.905639218062423	6.190965376167376
3	2.8509214263190947	3.8338136303913575
4	1.5843723206750875	2.8408070188785683
5	1.111796457933434	2.4918378875079252
6	0.7845774633555578	2.1101414759045314
7	0.6272908884934558	1.968301003115918
8	0.47506578992822246	1.703602382907252
9	0.3899123377839852	1.5730190472320875
>10	3.1713824506700363	29.1807339576156
>50	0.2830469843398038	8.469104016927494
>100	0.03091391574870218	1.9026449657807216
>500	0.0	0.0
>1k	0.0	0.0
>5k	2.8361390594984585E-4	1.074639709720578
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8453	0.9859784141529252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019012715190692867	0.0
2	0.0	0.0	0.0	0.07301815772621924	0.0
3	0.0	0.0	0.0	0.10112898202656881	0.0
4	0.0	0.0	0.0	0.14825252151761126	0.0
5	0.0	0.0	0.0	0.24786515202590395	0.0
6	0.0	0.0	0.0	0.36124158862316447	0.0
7	0.0	0.0	0.0	0.4355428130186943	0.0
8	0.0	0.0	0.0	0.6915729347583928	0.0
9	0.0	0.0	0.0	0.8483403532632467	0.0
10	0.0	0.0	0.0	1.0633123415850072	0.0
11	0.0	0.0	0.0	1.2025833964174446	0.0
12	0.0	0.0	0.0	1.3229583784836718	0.0
13	0.0	0.0	0.0	1.3788300998109226	0.0
14	0.0	0.0	0.0	1.4021585847074782	0.0
15	0.0	0.0	0.0	1.4271200635467929	0.0
16	0.0	0.0	0.0	1.4744768878868009	0.0
17	0.0	0.0	0.0	1.5511109607719862	0.0
18	0.0	0.0	0.0	1.6559725003820038	0.0
19	0.0	0.0	0.0	1.701696330779253	0.0
20	0.0	0.0	0.0	1.75698483998409	0.0
21	0.0	0.0	0.0	1.8136730582827203	0.0
22	0.0	0.0	0.0	1.8765433250789378	0.0
23	0.0	0.0	0.0	1.9454789979482598	0.0
24	0.0	0.0	0.0	1.996918307145165	0.0
25	0.0	0.0	0.0	2.036810016318275	0.0
26	0.0	0.0	0.0	2.082183919442076	0.0
27	0.0	0.0	0.0	2.135722792279671	0.0
28	0.0	0.0	0.0	2.197893204528992	0.0
29	0.0	0.0	0.0	2.264729313757624	0.0
30	0.0	0.0	0.0	2.3553604775807426	0.0
31	0.0	0.0	0.0	2.4330443322862734	0.0
32	0.0	0.0	0.0	2.505829205163527	0.0
33	0.0	0.0	0.0	2.5826965628976777	0.0
34	0.0	0.0	0.0	2.6543150115301035	0.0
35	0.0	0.0	0.0	2.746812454144947	0.0
36	0.0	0.0	0.0	2.831028284621513	0.0
37	0.0	0.0	0.0	2.9154773999470445	0.0
38	0.0	0.0	0.0	3.0133403940880954	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	40	8.309144E-9	44.000004	1
CTATCGA	20	7.8553596E-4	44.000004	38
TTGCTCG	20	7.8553596E-4	44.000004	1
CGTAAGA	20	7.8553596E-4	44.000004	26
TATAGCG	65	0.0	44.000004	1
TCGAACG	20	7.8553596E-4	44.000004	1
CGAACGG	55	1.8189894E-12	44.0	2
ACGTTAG	30	2.52696E-6	44.0	1
TCGACAG	35	1.4455145E-7	44.0	1
ACGACGT	35	1.4455145E-7	44.0	26
CGTTTTT	11555	0.0	43.028996	1
TATACGG	115	0.0	42.08696	2
TACGGGA	215	0.0	40.930233	4
CGCATGG	140	0.0	40.857143	2
CGTTAGG	135	0.0	40.74074	2
GCGGAAT	535	0.0	40.29907	8
CGTAGGC	115	0.0	40.173916	3
ATACGAA	230	0.0	40.173916	19
ATGGGAC	340	0.0	40.117645	5
TCTAGCG	55	7.8216544E-11	40.0	1
>>END_MODULE