FastQCFastQC Report
Thu 26 May 2016
SRR1546001_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546001_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences894215
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176001.9682067511728165No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC15540.17378370973423618No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT14390.1609232678941865No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10400.11630312620566642No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATG10190.1139546976957443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG408.309144E-944.0000041
CGCTAAC207.855541E-444.00000419
ACTCGAC207.855541E-444.00000420
ATACGAG207.855541E-444.00000435
GACTCGA207.855541E-444.00000429
TATCCGG207.855541E-444.0000042
ACGTTAG254.442044E-544.01
CGACCCG950.044.032
CTAAGCG302.5270838E-644.01
TCGCTAT302.5270838E-644.023
CGGTCTA600.044.031
TAGTGCG551.8189894E-1244.01
ATCGACG351.4456054E-743.9999961
CGTTTTT139300.043.1945421
GCGCGAC1000.041.8000039
GACGGTC650.040.61538729
CACAACG603.6379788E-1240.33333212
CGCGACC1050.039.8095210
GCCCTAC4700.039.7872326
CGTAAGG1550.039.7419362
Produced by FastQC (version 0.11.5)