##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546001_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	894215
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.105832489949286	31.0	28.0	33.0	25.0	33.0
2	28.86061629473896	31.0	28.0	33.0	16.0	33.0
3	28.308988330546903	31.0	27.0	33.0	16.0	34.0
4	32.05942642429393	35.0	32.0	35.0	25.0	37.0
5	33.668253160593366	35.0	33.0	35.0	28.0	37.0
6	33.50760499432463	35.0	33.0	37.0	28.0	37.0
7	34.238705456741386	35.0	33.0	37.0	30.0	37.0
8	34.717904530789575	35.0	35.0	37.0	32.0	37.0
9	36.538779823644205	39.0	37.0	39.0	32.0	39.0
10	36.331344251662074	38.0	35.0	39.0	32.0	39.0
11	36.38463904094653	39.0	35.0	39.0	32.0	39.0
12	35.29132814815229	37.0	34.0	39.0	30.0	39.0
13	35.257790352432025	37.0	34.0	39.0	27.0	39.0
14	36.12284294045615	38.0	34.0	40.0	29.0	41.0
15	36.69581252830695	38.0	35.0	40.0	31.0	41.0
16	36.37995448521888	38.0	35.0	40.0	31.0	41.0
17	35.963446151093414	38.0	34.0	40.0	30.0	41.0
18	35.54075138529325	37.0	34.0	39.0	29.0	40.0
19	34.96694419127391	37.0	34.0	39.0	27.0	40.0
20	35.31511884725709	35.0	34.0	39.0	30.0	40.0
21	35.498136354232486	35.0	34.0	39.0	30.0	40.0
22	35.79367825411115	36.0	34.0	40.0	31.0	41.0
23	36.10451960658231	36.0	35.0	40.0	32.0	41.0
24	35.77396487421929	36.0	35.0	40.0	31.0	41.0
25	35.41636966501345	35.0	34.0	40.0	30.0	41.0
26	35.47588555325061	35.0	34.0	40.0	30.0	41.0
27	35.78058296942011	36.0	35.0	40.0	31.0	41.0
28	35.729617597557635	36.0	35.0	40.0	31.0	41.0
29	35.87146044295835	36.0	35.0	40.0	31.0	41.0
30	35.49989991221351	36.0	35.0	40.0	30.0	41.0
31	34.816057659511415	35.0	34.0	40.0	29.0	41.0
32	34.60625576623072	35.0	34.0	40.0	25.0	41.0
33	34.139100775540555	36.0	33.0	40.0	23.0	41.0
34	33.64572390308818	36.0	33.0	40.0	18.0	41.0
35	33.25002040896205	36.0	33.0	40.0	15.0	41.0
36	32.805153123130346	35.0	33.0	40.0	14.0	41.0
37	32.65464233992944	35.0	32.0	40.0	11.0	41.0
38	32.51486163842029	35.0	32.0	40.0	10.0	41.0
39	32.33510956537298	35.0	32.0	40.0	10.0	40.0
40	32.17902629680781	35.0	31.0	39.0	10.0	40.0
41	31.886022936318447	35.0	31.0	39.0	10.0	40.0
42	32.03901410734555	35.0	31.0	39.0	10.0	40.0
43	31.81291523850528	35.0	31.0	39.0	10.0	40.0
44	31.545052364364274	35.0	31.0	39.0	9.0	40.0
45	31.517506416242178	35.0	30.0	39.0	10.0	40.0
46	31.36801664029344	35.0	30.0	39.0	10.0	40.0
47	31.36515938560637	35.0	30.0	39.0	10.0	40.0
48	31.38807781126463	35.0	30.0	39.0	9.0	40.0
49	31.377989633365576	35.0	30.0	39.0	9.0	40.0
50	31.510764189820122	35.0	30.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	3.0
12	7.0
13	4.0
14	37.0
15	102.0
16	240.0
17	620.0
18	1265.0
19	2029.0
20	3109.0
21	4546.0
22	6525.0
23	9364.0
24	13654.0
25	21269.0
26	30088.0
27	33655.0
28	30098.0
29	27678.0
30	28618.0
31	33476.0
32	44255.0
33	66815.0
34	107375.0
35	77760.0
36	78240.0
37	112663.0
38	130618.0
39	30098.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.704427906040497	21.156209636384986	26.570791140833023	25.5685713167415
2	40.32654339280822	20.320840066426978	27.6546468131266	11.697969727638208
3	26.547754175449978	18.55135509916519	42.73927411193058	12.161616613454258
4	23.644425557611985	23.697880263694973	39.76784106730484	12.8898531113882
5	19.48748343519176	28.26221881762216	39.362569404449715	12.887728342736365
6	17.48114267821497	35.82907913644929	37.19362793064308	9.496150254692663
7	72.6871054500316	4.880705423192409	20.20643804901506	2.225751077760941
8	73.82083727067875	3.747644582119513	19.634987111600676	2.796531035601058
9	69.96304020845099	5.36638280503011	20.827541474924935	3.843035511593968
10	40.41444171703673	25.166654551757688	25.28172754874387	9.137176182461712
11	36.372908081389824	18.650212756440006	33.877199554916885	11.09967960725329
12	33.113624799405066	19.86837617351532	35.02412730719122	11.993871719888393
13	18.763496474561485	33.84510436528128	35.101401788160565	12.289997371996668
14	13.407625682861504	35.87246914891832	37.427687972131984	13.292217196088188
15	13.039817046236085	21.004568252601445	53.351151568694334	12.60446313246814
16	14.395754935893493	18.760253406619213	50.51883495579922	16.325156701688073
17	15.114597719787747	18.288442935982957	38.59843549929268	27.998523844936617
18	18.417830163886762	20.914880649508227	42.73681385349161	17.930475333113403
19	23.148571652231286	22.675195562588417	36.47187756859368	17.704355216586613
20	26.063866072476976	21.203066376654384	36.21176115363755	16.521306397231093
21	19.449349429387787	25.30610647327544	37.57105394116628	17.673490156170494
22	19.071476099148416	22.177776038201102	36.711417276605744	22.03933058604474
23	16.50699216631347	26.74066080305072	35.97076765654792	20.78157937408789
24	16.89996253697377	21.183048819355523	46.0376978690807	15.879290774590004
25	14.918671684102817	23.057206600202413	43.82872128067635	18.195400435018424
26	14.798790000167743	29.48194785370409	38.10358806327338	17.61567408285479
27	15.452883255145574	29.224515357045007	38.68991238125059	16.632689006558827
28	13.598072052023284	25.26607135867772	45.540166514764344	15.595690074534648
29	14.574011842789487	21.914975704947913	44.762165698405866	18.748846753856736
30	16.678091957750652	26.774880761338153	40.250275381200275	16.29675189971092
31	23.919639012989048	24.588158328813538	35.276191967256196	16.216010690941214
32	25.5097487740644	23.898838646186878	35.17599235083285	15.415420228915867
33	22.949514378533127	26.222888231577418	33.81379198514898	17.01380540474047
34	18.433374524023865	25.5217145764721	35.360288073897216	20.684622825606816
35	18.40150299424635	26.07739749389129	36.13180275437115	19.389296757491206
36	26.338408548279773	24.821211900941048	31.87197709723053	16.96840245354864
37	19.10312396906784	30.533820166291104	33.29143438658488	17.071621478056173
38	18.807445636675745	30.490430153822068	31.07876741052208	19.62335679898011
39	19.895215356485856	29.232790771794253	31.98883937308142	18.883154498638472
40	22.239617988962383	25.55548721504336	31.21408162466521	20.99081317132904
41	17.59297260725888	25.14104549800663	31.62986530085047	25.636116593884022
42	21.1623602824824	24.95115827849007	29.726631738452163	24.159849700575364
43	21.20899336289371	25.784291249867202	30.02577679864462	22.980938588594466
44	19.153559267066644	28.626225236660087	31.115559457177522	21.104656039095744
45	16.69408363760393	33.67646483228307	28.27340181052655	21.356049719586455
46	20.85370967832121	30.067713022036084	29.869997707486455	19.208579592156248
47	19.966898341003002	26.151652566776445	31.386970694967093	22.494478397253456
48	20.95156086623463	24.143522530934955	33.15187063513808	21.753045967692334
49	20.158015689739045	23.4476048824947	34.57624844137037	21.81813098639589
50	18.7706535900203	29.554301817795498	31.3133866016562	20.361657990528002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	582.5
2	785.0
3	13941.5
4	27098.0
5	19961.5
6	12825.0
7	13268.0
8	13711.0
9	14380.5
10	15050.0
11	15316.0
12	15582.0
13	14934.0
14	14286.0
15	13257.5
16	12229.0
17	11567.0
18	10905.0
19	10024.0
20	9143.0
21	8586.0
22	8029.0
23	7534.5
24	7040.0
25	7369.0
26	7698.0
27	8014.5
28	8331.0
29	9866.5
30	11402.0
31	13164.5
32	14927.0
33	17662.0
34	20397.0
35	22690.5
36	24984.0
37	28209.0
38	31434.0
39	38911.5
40	46389.0
41	58288.0
42	70187.0
43	80994.0
44	91801.0
45	90737.5
46	89674.0
47	81074.5
48	72475.0
49	66636.5
50	60798.0
51	55137.5
52	49477.0
53	44734.0
54	39991.0
55	34716.5
56	29442.0
57	26207.5
58	22973.0
59	20318.5
60	17664.0
61	15435.0
62	13206.0
63	10943.0
64	8680.0
65	6996.0
66	5312.0
67	4453.0
68	3594.0
69	2940.0
70	2286.0
71	1994.5
72	1703.0
73	1364.0
74	1025.0
75	812.5
76	600.0
77	451.0
78	302.0
79	248.0
80	194.0
81	148.5
82	103.0
83	77.5
84	52.0
85	43.5
86	35.0
87	21.0
88	7.0
89	6.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	894215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.554900272789983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48408168828009	24.450024704023907
2	7.111127674060468	4.487818491641101
3	3.2885130645272582	3.113061053907738
4	2.0463205138910094	2.582857589679806
5	1.410501977852611	2.225412462285609
6	1.0665011035108307	2.0192001577262837
7	0.8004486879908312	1.7680654966125389
8	0.648716262238417	1.6376141568216258
9	0.5230803568419756	1.4855173645323543
>10	4.730936161990452	32.034557610162686
>50	0.7711124233888839	16.5384816621464
>100	0.1165220658698919	5.051104094053914
>500	3.563365928742815E-4	0.0592567529844259
>1k	0.001425346371497126	0.5680552487235667
>5k	0.0	0.0
>10k+	3.563365928742815E-4	1.9789731546980944
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17600	1.9682067511728165	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	1554	0.17378370973423618	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	1439	0.1609232678941865	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1040	0.11630312620566642	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATG	1019	0.1139546976957443	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1182992904391002E-4	0.0	0.0	0.04886967899218868	0.0
2	1.1182992904391002E-4	0.0	0.0	0.2154962732676146	0.0
3	1.1182992904391002E-4	0.0	0.0	0.28952768629468306	0.0
4	1.1182992904391002E-4	0.0	0.0	0.4459777570271132	0.0
5	1.1182992904391002E-4	0.0	0.0	0.8657873106579513	0.0
6	1.1182992904391002E-4	0.0	0.0	1.1291467935563595	0.0
7	1.1182992904391002E-4	0.0	0.0	1.3393870601589104	0.0
8	1.1182992904391002E-4	0.0	0.0	1.8608500192906627	0.0
9	2.2365985808782003E-4	0.0	0.0	2.1097834413424064	0.0
10	2.2365985808782003E-4	0.0	0.0	2.6716170048590104	0.0
11	2.2365985808782003E-4	0.0	0.0	2.9438110521518874	0.0
12	2.2365985808782003E-4	0.0	0.0	3.2956280089240284	0.0
13	2.2365985808782003E-4	0.0	0.0	3.414055903781529	0.0
14	2.2365985808782003E-4	0.0	0.0	3.460241664476664	0.0
15	2.2365985808782003E-4	0.0	0.0	3.5249911933930878	0.0
16	2.2365985808782003E-4	0.0	0.0	3.6636603054075363	0.0
17	2.2365985808782003E-4	0.0	0.0	3.8286094507473036	0.0
18	2.2365985808782003E-4	0.0	0.0	4.086041947406384	0.0
19	2.2365985808782003E-4	0.0	0.0	4.196082597585592	0.0
20	2.2365985808782003E-4	0.0	0.0	4.322338587476167	0.0
21	2.2365985808782003E-4	0.0	0.0	4.4510548358057065	0.0
22	2.2365985808782003E-4	0.0	0.0	4.587263689381189	0.0
23	2.2365985808782003E-4	0.0	0.0	4.734767365790106	0.0
24	2.2365985808782003E-4	0.0	0.0	4.848274743769675	0.0
25	2.2365985808782003E-4	0.0	0.0	4.941764564450384	0.0
26	2.2365985808782003E-4	0.0	0.0	5.039056602718586	0.0
27	2.2365985808782003E-4	0.0	0.0	5.142499287084203	0.0
28	2.2365985808782003E-4	0.0	0.0	5.240685964784755	0.0
29	2.2365985808782003E-4	0.0	0.0	5.351621254396314	0.0
30	2.2365985808782003E-4	0.0	0.0	5.506282046264042	0.0
31	2.2365985808782003E-4	0.0	0.0	5.630972417148001	0.0
32	2.2365985808782003E-4	0.0	0.0	5.735421570875014	0.0
33	2.2365985808782003E-4	0.0	0.0	5.845462221054221	0.0
34	3.354897871317301E-4	0.0	0.0	5.956285680736736	0.0
35	3.354897871317301E-4	0.0	0.0	6.105354976152268	0.0
36	3.354897871317301E-4	0.0	0.0	6.229038877674832	0.0
37	3.354897871317301E-4	0.0	0.0	6.350374350687474	0.0
38	3.354897871317301E-4	0.0	0.0	6.474058252210039	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	40	8.309144E-9	44.000004	1
CGCTAAC	20	7.855541E-4	44.000004	19
ACTCGAC	20	7.855541E-4	44.000004	20
ATACGAG	20	7.855541E-4	44.000004	35
GACTCGA	20	7.855541E-4	44.000004	29
TATCCGG	20	7.855541E-4	44.000004	2
ACGTTAG	25	4.442044E-5	44.0	1
CGACCCG	95	0.0	44.0	32
CTAAGCG	30	2.5270838E-6	44.0	1
TCGCTAT	30	2.5270838E-6	44.0	23
CGGTCTA	60	0.0	44.0	31
TAGTGCG	55	1.8189894E-12	44.0	1
ATCGACG	35	1.4456054E-7	43.999996	1
CGTTTTT	13930	0.0	43.194542	1
GCGCGAC	100	0.0	41.800003	9
GACGGTC	65	0.0	40.615387	29
CACAACG	60	3.6379788E-12	40.333332	12
CGCGACC	105	0.0	39.80952	10
GCCCTAC	470	0.0	39.78723	26
CGTAAGG	155	0.0	39.741936	2
>>END_MODULE