FastQCFastQC Report
Thu 26 May 2016
SRR1546000_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546000_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences894315
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181102.0250135578627217No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC15210.17007430267858642No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT14110.15777438598256766No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATG11110.12422915862978928No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10450.11684920861217804No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG254.4420456E-544.01
CGGGTCA207.855545E-444.06
ACGTTAG207.855545E-444.01
TATGCGA207.855545E-444.038
GTAATCG254.4420456E-544.037
CCGTACT207.855545E-444.022
TACGCGG207.855545E-444.02
CGTAGGC207.855545E-444.032
TCTGTCG207.855545E-444.034
TGCGAAT302.5270838E-644.027
CTACCGG254.4420456E-544.02
CATCGTA207.855545E-444.029
TAGTGCG454.802132E-1044.01
TTCGATG207.855545E-444.01
GCGTAGT207.855545E-444.043
CGTTTTT139900.043.512511
ACGGTCT950.041.6842130
TCGACGG750.041.0666662
TAACGGG2800.040.8571433
TCGATGG700.040.8571432
Produced by FastQC (version 0.11.5)