FastQCFastQC Report
Thu 26 May 2016
SRR1545999_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545999_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences882130
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180552.046750478954349No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC15090.17106322197408547No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT14750.16720891478580255No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA11670.13229342613900447No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATG10840.12288438212054913No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG302.5270438E-644.0000041
TCCGCGT207.855485E-444.011
ACGTTAG254.4419947E-544.01
GCGTTAG551.8189894E-1244.01
GCCGATT254.4419947E-544.09
GCGCTTA207.855485E-444.012
GCGCTAG207.855485E-444.01
CGTTTTT137250.043.3748661
CGTTGAT950.041.6842125
ACGGGAC2000.040.75
CGACGGT650.040.61538728
ACGGTCT650.040.61538730
AACGGGA2400.040.3333364
GGGACCG3450.040.1739167
GTTTTTT150750.040.05972
CGACAGG950.039.368422
TACGCGG452.3499524E-839.111112
AGGGCGA5700.038.9824526
GGCCGAT750.038.133338
GGGTACC1100.038.07