##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545999_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	882130
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.967508190402775	31.0	28.0	33.0	23.0	33.0
2	28.718689989003888	31.0	28.0	33.0	16.0	33.0
3	28.19484429732579	31.0	27.0	33.0	16.0	34.0
4	31.925436160203144	35.0	32.0	35.0	25.0	37.0
5	33.639215308401255	35.0	33.0	35.0	28.0	37.0
6	33.54004965254554	35.0	33.0	37.0	28.0	37.0
7	34.20813145454751	35.0	33.0	37.0	30.0	37.0
8	34.712179610714976	35.0	35.0	37.0	32.0	37.0
9	36.62864883860655	39.0	37.0	39.0	32.0	39.0
10	36.398402729756384	39.0	35.0	39.0	32.0	39.0
11	36.43290671442985	39.0	35.0	39.0	32.0	39.0
12	35.385660843639826	37.0	35.0	39.0	30.0	39.0
13	35.329144230442225	37.0	34.0	39.0	29.0	39.0
14	36.18845408273157	38.0	35.0	40.0	30.0	41.0
15	36.70101685692585	38.0	35.0	40.0	31.0	41.0
16	36.368801650550374	38.0	34.0	40.0	31.0	41.0
17	35.95702560847041	38.0	34.0	40.0	30.0	41.0
18	35.53211431421673	37.0	34.0	39.0	29.0	40.0
19	34.93011234171834	37.0	33.0	39.0	27.0	40.0
20	35.34734449570925	35.0	34.0	39.0	30.0	40.0
21	35.467966172786326	35.0	34.0	39.0	30.0	40.0
22	35.775753006926415	36.0	34.0	40.0	31.0	41.0
23	36.093199415052204	36.0	35.0	40.0	32.0	41.0
24	35.697833652636234	35.0	35.0	40.0	31.0	41.0
25	35.34847924909027	35.0	34.0	39.0	30.0	41.0
26	35.434441635586595	35.0	34.0	40.0	30.0	41.0
27	35.781194381780466	36.0	35.0	40.0	31.0	41.0
28	35.74270232278689	36.0	35.0	40.0	31.0	41.0
29	35.869189348508726	36.0	35.0	40.0	31.0	41.0
30	35.53665332774081	36.0	35.0	40.0	30.0	41.0
31	34.81176017140331	35.0	34.0	40.0	29.0	41.0
32	34.6647421581853	35.0	34.0	40.0	26.0	41.0
33	34.21718340834117	36.0	34.0	40.0	23.0	41.0
34	33.65211023318558	36.0	33.0	40.0	18.0	41.0
35	33.24542301021391	36.0	33.0	40.0	15.0	41.0
36	32.82162039608674	35.0	33.0	40.0	13.0	41.0
37	32.69818167390294	35.0	33.0	40.0	11.0	41.0
38	32.56339995238797	35.0	32.0	40.0	10.0	41.0
39	32.331465883713285	35.0	32.0	40.0	10.0	41.0
40	32.19733599356104	35.0	31.0	40.0	10.0	40.0
41	31.885066826884927	35.0	31.0	39.0	10.0	40.0
42	32.06805119426842	35.0	31.0	39.0	10.0	40.0
43	31.867658961831022	35.0	31.0	39.0	10.0	40.0
44	31.62321539909084	35.0	31.0	39.0	10.0	40.0
45	31.586811467697505	35.0	30.0	39.0	10.0	40.0
46	31.41075465067507	35.0	30.0	39.0	9.0	40.0
47	31.427135456225272	35.0	30.0	39.0	10.0	40.0
48	31.43560813032093	35.0	31.0	39.0	9.0	40.0
49	31.502832915783387	35.0	31.0	39.0	9.0	40.0
50	31.58156280820287	35.0	31.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	4.0
13	16.0
14	24.0
15	104.0
16	249.0
17	578.0
18	1200.0
19	2085.0
20	3001.0
21	4623.0
22	6504.0
23	9136.0
24	13364.0
25	20736.0
26	29654.0
27	33252.0
28	29781.0
29	27129.0
30	27868.0
31	33192.0
32	43099.0
33	66399.0
34	106232.0
35	75882.0
36	76834.0
37	111184.0
38	130059.0
39	29937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.654234636618185	21.069116796844003	26.471268407150873	25.805380159386935
2	40.581660299502346	20.262999784612244	27.591511455227685	11.563828460657726
3	26.599027354244843	18.52822146395656	42.62194914581751	12.250802035981092
4	23.46116785507805	23.682337070499813	39.87088070919253	12.985614365229614
5	19.437271150510696	28.221010508655187	39.49678618797683	12.844932152857288
6	17.555122260891252	35.83893530432022	37.20834797592192	9.397594458866607
7	72.36756487139083	4.930905875551223	20.38293675535352	2.31859249770442
8	73.57838413839231	3.830727897248705	19.768968292655277	2.821919671703717
9	69.87178760500153	5.375511545917268	21.0075612438076	3.7451396052735992
10	40.40141475746205	25.15502250235226	25.298425402151608	9.145137338034075
11	36.337387913346106	18.612449412218155	33.96868942219401	11.081473252241732
12	33.09806944554657	19.835965220545724	35.01263986033805	12.053325473569656
13	18.76242730663281	33.87403217212883	35.06535317923662	12.298187342001746
14	13.39734506251913	35.83031979413465	37.449015451237344	13.323319692108873
15	12.964755761622438	21.084307301644884	53.314704181923304	12.636232754809381
16	14.413068368607801	18.720143289537823	50.524299139582595	16.342489202271775
17	15.063426025642478	18.319748789860903	38.715495448516656	27.901329735979957
18	18.379603913255416	20.788205820003856	42.88506229240588	17.947127974334848
19	23.154523709657308	22.633738791334608	36.494620974232824	17.71711652477526
20	25.995714917302436	21.14654302653804	36.25667418634442	16.601067869815108
21	19.4931586047408	25.269064650334983	37.5339235713557	17.703853173568522
22	19.019872354414883	22.169181413170396	36.701279856710464	22.10966637570426
23	16.498475281421104	26.704794077970366	36.02598256492807	20.770748075680455
24	16.99171323954519	21.15606543253262	46.05738383231496	15.794837495607222
25	15.01139287860066	23.01191434369084	43.91144162425039	18.065251153458107
26	14.78387539251584	29.35927811093603	38.21137474068448	17.64547175586365
27	15.42720460703071	29.17619851949259	38.7558523120175	16.640744561459194
28	13.547322956933785	25.253760783557976	45.627515218845296	15.571401040662941
29	14.508632514481992	21.824107557842947	44.947230000113365	18.720029927561697
30	16.635529910557402	26.658769115663222	40.366499268815254	16.339201704964122
31	23.87720630746035	24.554657476789135	35.25886207248365	16.309274143266865
32	25.44488907530636	23.843651162527067	35.33980252343759	15.371657238728986
33	22.93460147597293	26.079035969755026	33.955652794939525	17.030709759332524
34	18.344461700656367	25.510298935531043	35.53387822656525	20.611361137247343
35	18.398988811172956	25.994694659517304	36.26370262886422	19.342613900445514
36	26.30496638817408	24.74453878680013	31.97204493668734	16.978449888338453
37	19.10149297722558	30.489270288959673	33.357781732851166	17.05145500096358
38	18.867400496525455	30.382029859544513	31.06786981510662	19.682699828823417
39	19.906249645743827	29.15738043145568	32.05638624692506	18.87998367587544
40	22.191740446419463	25.471189053767585	31.33222994343237	21.00484055638058
41	17.6544273519776	24.993821772301132	31.774455012299775	25.57729586342149
42	21.076598687268316	25.09471393105325	29.758312266899438	24.070375114779
43	21.26171879428202	25.805493521363065	30.10508655186877	22.82770113248614
44	19.09922573770306	28.673438155373926	31.161280083434413	21.066056023488603
45	16.653101016856926	33.648441839638146	28.425968961490938	21.272488182013987
46	20.957228526407672	30.159727024361487	29.844467368755172	19.038577080475665
47	19.90420913017356	26.294310362418237	31.401607472821468	22.39987303458674
48	20.993164272839604	24.347431784430867	33.15735775906046	21.502046183669073
49	20.131386530329998	23.5168285853559	34.69919399634974	21.652590887964358
50	18.82386949769308	29.588042578758234	31.37734800993051	20.210739913618177
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	357.0
1	573.5
2	790.0
3	14213.0
4	27636.0
5	20233.5
6	12831.0
7	13153.0
8	13475.0
9	14385.0
10	15295.0
11	15254.5
12	15214.0
13	14529.5
14	13845.0
15	13047.0
16	12249.0
17	11434.0
18	10619.0
19	9793.0
20	8967.0
21	8482.0
22	7997.0
23	7436.0
24	6875.0
25	7208.5
26	7542.0
27	7824.5
28	8107.0
29	9598.5
30	11090.0
31	12891.5
32	14693.0
33	17423.0
34	20153.0
35	22437.0
36	24721.0
37	27764.0
38	30807.0
39	38247.5
40	45688.0
41	57257.0
42	68826.0
43	79876.0
44	90926.0
45	89433.0
46	87940.0
47	79610.5
48	71281.0
49	65680.5
50	60080.0
51	54491.5
52	48903.0
53	44075.0
54	39247.0
55	34181.0
56	29115.0
57	25986.5
58	22858.0
59	20150.0
60	17442.0
61	15185.0
62	12928.0
63	10784.0
64	8640.0
65	6919.0
66	5198.0
67	4387.0
68	3576.0
69	2898.5
70	2221.0
71	1954.0
72	1687.0
73	1337.5
74	988.0
75	787.0
76	586.0
77	465.5
78	345.0
79	263.5
80	182.0
81	146.5
82	111.0
83	76.5
84	42.0
85	37.0
86	32.0
87	22.0
88	12.0
89	8.5
90	5.0
91	4.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	882130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.01195299789028
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8545659359919	23.834101864802403
2	7.35505680679848	4.561893519784948
3	3.2638825954372765	3.036581209209989
4	2.0999400219232873	2.6049296503309467
5	1.4978924482110059	2.3226285099907256
6	1.1069488097691331	2.0597186675778567
7	0.8618903015795107	1.8710231065344887
8	0.6518880695146222	1.6173057737338312
9	0.5507924414852121	1.5373034376239367
>10	4.855330135093962	32.38512687639065
>50	0.7877477445836321	16.66352582157421
>100	0.11184984126999747	4.781400724135722
>500	3.691413903300231E-4	0.05826927882263687
>1k	0.0014765655613200924	0.5992920916237799
>5k	0.0	0.0
>10k+	3.691413903300231E-4	2.0668994678638675
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18055	2.046750478954349	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	1509	0.17106322197408547	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	1475	0.16720891478580255	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1167	0.13229342613900447	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATG	1084	0.12288438212054913	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0489723736864181	0.0
2	0.0	0.0	0.0	0.19906363007719952	0.0
3	0.0	0.0	0.0	0.26447349030188294	0.0
4	0.0	0.0	0.0	0.4146781086687903	0.0
5	1.1336197612596783E-4	0.0	0.0	0.8078174418736468	0.0
6	1.1336197612596783E-4	0.0	0.0	1.071270674390396	0.0
7	1.1336197612596783E-4	0.0	0.0	1.2775894709396574	0.0
8	1.1336197612596783E-4	0.0	0.0	1.7820502647002143	0.0
9	1.1336197612596783E-4	0.0	0.0	2.0121750762359287	0.0
10	1.1336197612596783E-4	0.0	0.0	2.5382880074365457	0.0
11	1.1336197612596783E-4	0.0	0.0	2.7916520240780835	0.0
12	1.1336197612596783E-4	0.0	0.0	3.142393978211828	0.0
13	1.1336197612596783E-4	0.0	0.0	3.254849058528788	0.0
14	1.1336197612596783E-4	0.0	0.0	3.3031412603584505	0.0
15	1.1336197612596783E-4	0.0	0.0	3.375239477174566	0.0
16	2.2672395225193566E-4	0.0	0.0	3.5119540203824835	0.0
17	2.2672395225193566E-4	0.0	0.0	3.663178896534524	0.0
18	3.400859283779035E-4	0.0	0.0	3.898404996995908	0.0
19	3.400859283779035E-4	0.0	0.0	4.010293267432238	0.0
20	3.400859283779035E-4	0.0	0.0	4.126829378889733	0.0
21	3.400859283779035E-4	0.0	0.0	4.246312901726503	0.0
22	3.400859283779035E-4	0.0	0.0	4.37599900241461	0.0
23	3.400859283779035E-4	0.0	0.0	4.5197419881423375	0.0
24	3.400859283779035E-4	0.0	0.0	4.628002675342636	0.0
25	3.400859283779035E-4	0.0	0.0	4.7203926858853	0.0
26	3.400859283779035E-4	0.0	0.0	4.815730107807239	0.0
27	3.400859283779035E-4	0.0	0.0	4.911067529729178	0.0
28	3.400859283779035E-4	0.0	0.0	5.008898915125888	0.0
29	3.400859283779035E-4	0.0	0.0	5.107297110403229	0.0
30	3.400859283779035E-4	0.0	0.0	5.250700010202578	0.0
31	4.534479045038713E-4	0.0	0.0	5.368936551301962	0.0
32	4.534479045038713E-4	0.0	0.0	5.473116207361727	0.0
33	4.534479045038713E-4	0.0	0.0	5.5797898268962625	0.0
34	4.534479045038713E-4	0.0	0.0	5.690204391642955	0.0
35	5.668098806298392E-4	0.0	0.0	5.827712468683754	0.0
36	5.668098806298392E-4	0.0	0.0	5.945382199902508	0.0
37	6.80171856755807E-4	0.0	0.0	6.061578225431626	0.0
38	7.935338328817747E-4	0.0	0.0	6.17924795665038	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGCG	30	2.5270438E-6	44.000004	1
TCCGCGT	20	7.855485E-4	44.0	11
ACGTTAG	25	4.4419947E-5	44.0	1
GCGTTAG	55	1.8189894E-12	44.0	1
GCCGATT	25	4.4419947E-5	44.0	9
GCGCTTA	20	7.855485E-4	44.0	12
GCGCTAG	20	7.855485E-4	44.0	1
CGTTTTT	13725	0.0	43.374866	1
CGTTGAT	95	0.0	41.68421	25
ACGGGAC	200	0.0	40.7	5
CGACGGT	65	0.0	40.615387	28
ACGGTCT	65	0.0	40.615387	30
AACGGGA	240	0.0	40.333336	4
GGGACCG	345	0.0	40.173916	7
GTTTTTT	15075	0.0	40.0597	2
CGACAGG	95	0.0	39.36842	2
TACGCGG	45	2.3499524E-8	39.11111	2
AGGGCGA	570	0.0	38.982452	6
GGCCGAT	75	0.0	38.13333	8
GGGTACC	110	0.0	38.0	7
>>END_MODULE