FastQCFastQC Report
Thu 26 May 2016
SRR1545998_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545998_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences781504
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90151.1535449594627796No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT17000.21752927688150026No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT207.8549323E-444.00000421
TCGTACC207.8549323E-444.00000430
ACGACGT207.8549323E-444.00000426
ACTCGCG254.4415276E-544.01
TATAGCG254.4415276E-544.01
CGGTCTA302.5266727E-644.031
TAGGCGT454.802132E-1044.05
GCTACCG302.5266727E-644.01
TACGTAT302.5266727E-644.012
TGCGTAA254.4415276E-544.01
CGTTTTT128000.043.3812521
TTAGGCG1700.042.7058834
CACGGGA1400.040.8571434
TCGACGG650.040.6153872
TCAGGCG557.8216544E-1140.04
ATACTCG557.8216544E-1140.044
GTTTTTT141650.039.7599682
GTACCGG501.3460522E-939.6000022
ACGCAAT501.3460522E-939.60000222
CGGGAAT11450.039.1965079
Produced by FastQC (version 0.11.5)