##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545998_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	781504
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.02583863934158	28.0	25.0	30.0	16.0	33.0
2	26.13449067439194	30.0	25.0	31.0	10.0	33.0
3	26.461900386946198	30.0	25.0	31.0	16.0	33.0
4	27.65389300630579	32.0	19.0	35.0	10.0	35.0
5	31.24520028048481	33.0	30.0	35.0	19.0	37.0
6	29.92891271189911	33.0	28.0	35.0	17.0	36.0
7	30.030899393989028	32.0	28.0	35.0	17.0	35.0
8	32.433270974940626	33.0	32.0	35.0	28.0	35.0
9	27.14978426214069	31.0	23.0	34.0	10.0	35.0
10	31.865344259274426	34.0	30.0	37.0	17.0	39.0
11	33.8493021148964	35.0	32.0	38.0	27.0	39.0
12	33.347070520432396	35.0	31.0	39.0	25.0	39.0
13	33.22574548562771	35.0	31.0	39.0	23.0	39.0
14	33.13023221890099	36.0	31.0	39.0	22.0	40.0
15	32.03987951437229	34.0	27.0	38.0	18.0	40.0
16	30.717507523953813	34.0	27.0	38.0	17.0	40.0
17	30.981170921709932	33.0	27.0	38.0	17.0	40.0
18	31.801916560887726	34.0	29.0	38.0	18.0	39.0
19	31.427356737777412	34.0	29.0	37.0	18.0	39.0
20	32.13633711407747	34.0	30.0	37.0	23.0	39.0
21	32.80014561665711	34.0	31.0	37.0	24.0	39.0
22	33.12567945909426	35.0	31.0	37.0	25.0	39.0
23	34.30731512570633	35.0	33.0	38.0	28.0	40.0
24	33.95314035500778	35.0	33.0	38.0	27.0	40.0
25	32.73002313487839	35.0	31.0	37.0	23.0	39.0
26	26.327957374498403	33.0	20.0	36.0	0.0	39.0
27	29.62795967774957	34.0	24.0	37.0	15.0	39.0
28	31.1940220395545	34.0	27.0	36.0	19.0	39.0
29	32.489213107034644	34.0	30.0	37.0	23.0	39.0
30	32.21362654573745	34.0	30.0	37.0	22.0	39.0
31	31.11285418884612	34.0	29.0	36.0	19.0	38.0
32	31.2537248689706	34.0	30.0	37.0	18.0	39.0
33	30.945542185324708	34.0	29.0	37.0	17.0	39.0
34	30.698256182949798	34.0	29.0	37.0	15.0	39.0
35	29.831735985996232	34.0	27.0	37.0	10.0	39.0
36	29.483700659241666	34.0	25.0	37.0	9.0	39.0
37	29.447851834411598	34.0	25.0	37.0	9.0	39.0
38	29.192471183768735	33.0	25.0	37.0	9.0	39.0
39	29.057413653672917	33.0	24.0	36.0	8.0	39.0
40	29.019708152485464	33.0	24.0	36.0	9.0	38.0
41	28.424559567193516	33.0	23.0	36.0	8.0	38.0
42	28.59349536278765	33.0	23.0	36.0	8.0	38.0
43	28.18908412496929	33.0	23.0	36.0	8.0	38.0
44	27.981457548521824	32.0	23.0	35.0	7.0	38.0
45	27.467882442060436	31.0	23.0	35.0	7.0	38.0
46	27.010756182949798	31.0	21.0	35.0	7.0	38.0
47	27.176683676603062	31.0	23.0	35.0	7.0	38.0
48	27.25807545450823	31.0	23.0	35.0	7.0	38.0
49	27.116297549340757	31.0	22.0	35.0	7.0	38.0
50	28.867291786094505	34.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	0.0
11	9.0
12	30.0
13	111.0
14	284.0
15	654.0
16	1309.0
17	2324.0
18	3876.0
19	5999.0
20	8987.0
21	12596.0
22	17125.0
23	22296.0
24	29142.0
25	36089.0
26	41951.0
27	44193.0
28	46871.0
29	53740.0
30	63235.0
31	73902.0
32	80308.0
33	79496.0
34	69818.0
35	53880.0
36	27413.0
37	5707.0
38	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.81292482188191	21.90059167963312	26.141388911637048	27.14509458684792
2	40.32647305707968	17.65621161248055	29.286606338547212	12.730708991892556
3	27.771834821062974	14.468767914175743	44.436368847760214	13.323028417001066
4	24.76097371222668	23.389003767095243	38.94682560805831	12.90319691261977
5	18.64174719515191	28.52333449348948	40.27682519859143	12.558093112767176
6	17.42281549422652	34.344545901236586	38.56602141511752	9.666617189419375
7	74.15560253050528	3.6393927606256655	20.670527598067316	1.534477110801736
8	76.06691712390467	2.6097115305871754	19.50700188354762	1.8163694619605275
9	64.02372860535583	3.5700393088199167	19.137330071247234	13.268902014577023
10	41.387376136270575	25.03019818196708	25.43480263696667	8.147623044795676
11	37.43627671771353	17.929914626156744	34.324968266317256	10.308840389812463
12	33.54992424862829	19.67296392596839	35.54837851117845	11.228733314224879
13	18.136183563999673	34.65292564081566	35.327394357546474	11.883496437638195
14	12.806972197199245	36.00544590942592	38.31752620587994	12.870055687494883
15	11.615295635083122	19.187745680124475	53.48174801408566	15.715210670706739
16	12.83269183523053	16.643292932601753	50.43326713618869	20.090748095979034
17	14.467488330194087	17.250967365490133	39.472990541315205	28.80855376300057
18	17.88640877078044	20.18518139382524	44.08742629596266	17.840983539431658
19	23.610243837523544	21.802447588240113	37.343634837441655	17.243673736794694
20	26.375296863483744	20.682171812300385	36.97805769388257	15.964473630333305
21	19.14731082630415	24.998080624027516	38.2831053967734	17.57150315289493
22	18.66529154041438	21.661437433461632	37.29616227172222	22.37710875440177
23	15.65890897551388	26.637739538121362	36.99622778642208	20.707123699942677
24	16.07490172795021	20.574046965850464	48.14549893538613	15.205552370813201
25	14.331212636147736	21.778775284579478	45.735402505937266	18.154609573335517
26	11.670573663090655	41.13120342314307	31.86547989517648	15.332743018589795
27	14.36179469330931	30.14200823028417	39.474142166898694	16.022054909507823
28	12.875813815412332	24.955214560642045	47.07474305953648	15.09422856440914
29	13.639213618868235	21.639556547375317	46.241094095487675	18.480135738268775
30	16.059930595364836	26.61368335926624	41.20298808451396	16.123397960854966
31	24.554064982392926	24.113632175906968	35.91062873638523	15.421674105314882
32	25.662952460895916	23.155607648841208	35.93263758086971	15.24880230939317
33	23.31018139382524	25.32821329129473	34.5363555400868	16.825249774793217
34	17.90944128245025	24.757646793874375	35.64511506019163	21.687796863483744
35	18.096516460568342	25.316952952256162	36.550420727213165	20.036109859962327
36	26.544329907460483	24.005763246253377	31.820694455818526	17.62921239046761
37	18.334774997952668	30.890053025960203	32.855877896978136	17.919294079109
38	18.27936901154697	30.806112316763574	30.97450556874949	19.94001310293997
39	19.23445049545492	29.260758742117766	31.977955327164032	19.526835435263287
40	22.205772459258046	24.892259028744572	31.119994267463763	21.781974244533618
41	16.947705961837688	24.27511567439194	31.854347514536073	26.9228308492343
42	20.262468266317253	24.350611129309637	28.976691098190155	26.410229506182947
43	20.696887028089428	24.99078699533208	30.153012652526414	24.159313324052086
44	18.400417656211612	27.944578658586522	31.36554643354353	22.28945725165834
45	15.860315494226517	34.5055175661289	27.791668372778645	21.842498566865938
46	19.99426746376218	29.70259909098354	29.54418659405454	20.758946851199738
47	19.17981225943821	25.698396937187784	30.777577593972648	24.34421320940136
48	20.04967345016788	23.730780648595527	33.69067848661043	22.52886741462616
49	19.635088240111376	23.14716239456228	34.30846163295389	22.90928773237245
50	18.238294365735815	30.12115101138318	31.016092048153304	20.624462574727705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	183.0
1	341.0
2	499.0
3	8065.0
4	15631.0
5	13135.0
6	10639.0
7	10716.0
8	10793.0
9	11896.5
10	13000.0
11	13579.5
12	14159.0
13	14209.0
14	14259.0
15	13772.5
16	13286.0
17	12795.0
18	12304.0
19	11518.0
20	10732.0
21	9998.5
22	9265.0
23	8755.5
24	8246.0
25	8306.0
26	8366.0
27	8402.5
28	8439.0
29	9492.5
30	10546.0
31	12243.5
32	13941.0
33	16081.5
34	18222.0
35	20267.5
36	22313.0
37	24536.5
38	26760.0
39	32806.0
40	38852.0
41	49199.5
42	59547.0
43	66740.0
44	73933.0
45	74740.5
46	75548.0
47	68867.0
48	62186.0
49	57485.5
50	52785.0
51	48482.5
52	44180.0
53	39712.0
54	35244.0
55	30926.5
56	26609.0
57	23650.5
58	20692.0
59	18239.5
60	15787.0
61	13820.0
62	11853.0
63	10041.5
64	8230.0
65	6660.5
66	5091.0
67	4260.0
68	3429.0
69	2778.0
70	2127.0
71	1842.5
72	1558.0
73	1267.0
74	976.0
75	784.5
76	593.0
77	447.0
78	301.0
79	240.5
80	180.0
81	150.0
82	120.0
83	83.0
84	46.0
85	38.0
86	30.0
87	22.5
88	15.0
89	10.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	781504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.373446398222676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.58278735915962	38.2964846301581
2	6.824581624243913	6.32958740284341
3	2.7821916756917675	3.8705944962682053
4	1.5678342497609883	2.908235101703554
5	1.0114979634738797	2.345332329553368
6	0.7069432203326055	1.9670036120808605
7	0.5491051430220967	1.7824728541846517
8	0.4553941519829452	1.6894557037636144
9	0.33290584347516805	1.3894192159245733
>10	2.891356084466872	27.683569916250374
>50	0.26268024169861853	7.920825374014186
>100	0.031821825002732645	2.222302140770602
>500	3.0020589625207267E-4	0.1030197070832416
>1k	3.0020589625207267E-4	0.23666689465069013
>5k	3.0020589625207267E-4	1.2550306207505715
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9015	1.1535449594627796	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	1700	0.21752927688150026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035956309884530344	0.0
2	0.0	0.0	0.0	0.14548869871427403	0.0
3	0.0	0.0	0.0	0.2085721890099091	0.0
4	0.0	0.0	0.0	0.2904655638358857	0.0
5	0.0	0.0	0.0	0.5364016051101466	0.0
6	0.0	0.0	0.0	0.6818903038244206	0.0
7	0.0	0.0	0.0	0.7781150192449431	0.0
8	0.0	0.0	0.0	1.0743387109982803	0.0
9	0.0	0.0	0.0	1.2536084268282697	0.0
10	0.0	0.0	0.0	1.574656047825731	0.0
11	0.0	0.0	0.0	1.7573806404061911	0.0
12	0.0	0.0	0.0	1.9839949635574483	0.0
13	0.0	0.0	0.0	2.067039963966915	0.0
14	0.0	0.0	0.0	2.0926316436000327	0.0
15	0.0	0.0	0.0	2.1346019981983457	0.0
16	0.0	0.0	0.0	2.223149209728933	0.0
17	0.0	0.0	0.0	2.3398472688559497	0.0
18	0.0	0.0	0.0	2.497364056997789	0.0
19	0.0	0.0	0.0	2.573115428711817	0.0
20	0.0	0.0	0.0	2.6529614691671446	0.0
21	0.0	0.0	0.0	2.727945090492179	0.0
22	0.0	0.0	0.0	2.808430922938334	0.0
23	0.0	0.0	0.0	2.8930114241257883	0.0
24	0.0	0.0	0.0	2.9488012857259847	0.0
25	0.0	0.0	0.0	3.0013921873720415	0.0
26	0.0	0.0	0.0	3.0570540905740726	0.0
27	0.0	0.0	0.0	3.1289667103431333	0.0
28	0.0	0.0	0.0	3.1978083285562198	0.0
29	0.0	0.0	0.0	3.2797017033821962	0.0
30	0.0	0.0	0.0	3.3912814265825895	0.0
31	0.0	0.0	0.0	3.4814920972893293	0.0
32	0.0	0.0	0.0	3.5594187617721724	0.0
33	0.0	0.0	0.0	3.6387529686348374	0.0
34	0.0	0.0	0.0	3.727812013758087	0.0
35	0.0	0.0	0.0	3.8370884857914995	0.0
36	0.0	0.0	0.0	3.929474449267054	0.0
37	0.0	0.0	0.0	4.0219883711407745	0.0
38	0.0	0.0	0.0	4.113094750634674	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCGT	20	7.8549323E-4	44.000004	21
TCGTACC	20	7.8549323E-4	44.000004	30
ACGACGT	20	7.8549323E-4	44.000004	26
ACTCGCG	25	4.4415276E-5	44.0	1
TATAGCG	25	4.4415276E-5	44.0	1
CGGTCTA	30	2.5266727E-6	44.0	31
TAGGCGT	45	4.802132E-10	44.0	5
GCTACCG	30	2.5266727E-6	44.0	1
TACGTAT	30	2.5266727E-6	44.0	12
TGCGTAA	25	4.4415276E-5	44.0	1
CGTTTTT	12800	0.0	43.381252	1
TTAGGCG	170	0.0	42.705883	4
CACGGGA	140	0.0	40.857143	4
TCGACGG	65	0.0	40.615387	2
TCAGGCG	55	7.8216544E-11	40.0	4
ATACTCG	55	7.8216544E-11	40.0	44
GTTTTTT	14165	0.0	39.759968	2
GTACCGG	50	1.3460522E-9	39.600002	2
ACGCAAT	50	1.3460522E-9	39.600002	22
CGGGAAT	1145	0.0	39.196507	9
>>END_MODULE