FastQCFastQC Report
Thu 26 May 2016
SRR1545997_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545997_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences397052
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71891.810594078357495No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5960.15010628330797982No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC5240.13197263834459969No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT4890.12315767204295659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC302.523515E-644.025
CGGGTAT254.4375607E-544.06
TCGTTCA207.850251E-444.016
CGTATGG207.850251E-444.02
CAATTGC207.850251E-444.025
CGCGATC207.850251E-444.041
TCGATGG408.289135E-944.02
ACGGGTA408.289135E-944.05
TCTAGCG207.850251E-444.01
CTAACGG207.850251E-444.02
TATTCAC302.523515E-644.022
AATCTAT254.4375607E-544.030
GCGATTC254.4375607E-544.09
AATTCGC302.523515E-644.013
GCGATAT408.289135E-944.09
CCGACTT207.850251E-444.012
CGGTCTA254.4375607E-544.031
CAGTACG302.523515E-644.011
GCTAACG207.850251E-444.01
CTTAGTA207.850251E-444.030
Produced by FastQC (version 0.11.5)