FastQCFastQC Report
Thu 26 May 2016
SRR1545996_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545996_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences400639
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74531.8602782055666074No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6520.16274002281355535No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT4800.11980860575231068No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC4480.1118213653688233No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATG4010.10009010605557622No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG408.289135E-944.02
CGTATGG207.8503345E-444.02
CGAAAGT207.8503345E-444.030
GCGAAAG254.437632E-544.01
TTTCGAG254.437632E-544.01
CTATACG207.8503345E-444.01
CGACAGG454.783942E-1044.02
ATACTCC207.8503345E-444.044
ATACTAG207.8503345E-444.01
CTAAACG207.8503345E-444.016
GTCGTCT207.8503345E-444.012
ACTAACG254.437632E-544.01
CGGTCTA408.289135E-944.031
TATACGG207.8503345E-444.02
TCGACCC207.8503345E-444.029
TTCGAAG254.437632E-544.01
ACGACTG254.437632E-544.01
TACGTAG351.4429315E-743.9999961
CGTTTTT55650.043.6442031
GCATAGG900.041.5555572
Produced by FastQC (version 0.11.5)