##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545996_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	400639
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.007565414250735	31.0	28.0	33.0	23.0	33.0
2	28.794001582472	31.0	28.0	33.0	16.0	33.0
3	28.157635677005985	31.0	27.0	33.0	16.0	34.0
4	32.11917961057211	35.0	32.0	35.0	25.0	37.0
5	33.66424636642963	35.0	33.0	35.0	28.0	37.0
6	33.517311095524896	35.0	33.0	37.0	28.0	37.0
7	34.32118939993361	35.0	35.0	37.0	31.0	37.0
8	34.69624774422859	35.0	35.0	37.0	32.0	37.0
9	36.570708293501134	39.0	37.0	39.0	32.0	39.0
10	36.33328507708935	39.0	35.0	39.0	32.0	39.0
11	36.37869004265686	38.0	35.0	39.0	32.0	39.0
12	34.95388616684846	37.0	33.0	39.0	29.0	39.0
13	34.75404291644099	37.0	33.0	39.0	26.0	39.0
14	35.48647036359416	38.0	33.0	40.0	27.0	41.0
15	36.214063034302704	38.0	34.0	40.0	31.0	41.0
16	35.94562686108941	37.0	34.0	40.0	31.0	41.0
17	35.51705899825029	36.0	34.0	40.0	29.0	41.0
18	35.16553805296039	36.0	33.0	39.0	29.0	40.0
19	34.59839156946777	36.0	33.0	39.0	27.0	40.0
20	34.8769091376526	35.0	34.0	39.0	29.0	40.0
21	35.00561852440726	35.0	34.0	39.0	30.0	40.0
22	35.42555267959435	35.0	34.0	39.0	30.0	40.0
23	35.82030705947249	35.0	34.0	40.0	31.0	41.0
24	35.369981454626235	35.0	34.0	39.0	31.0	41.0
25	35.05243373710498	35.0	34.0	39.0	30.0	41.0
26	35.142230287116334	35.0	34.0	39.0	30.0	41.0
27	35.46612286871722	35.0	34.0	40.0	31.0	41.0
28	35.40848494529988	35.0	34.0	40.0	31.0	41.0
29	35.594338044973156	36.0	35.0	40.0	31.0	41.0
30	35.25609339080818	36.0	34.0	40.0	30.0	41.0
31	34.54421561555415	35.0	34.0	39.0	28.0	40.0
32	34.40166583882248	35.0	34.0	39.0	26.0	40.0
33	34.023315253881925	35.0	33.0	40.0	23.0	41.0
34	33.55345335826018	35.0	33.0	40.0	21.0	41.0
35	33.24788400530153	35.0	33.0	40.0	18.0	41.0
36	32.82290291259713	35.0	33.0	40.0	15.0	41.0
37	32.71425397926812	35.0	33.0	40.0	15.0	41.0
38	32.58500794980019	35.0	32.0	39.0	15.0	40.0
39	32.41103587019736	35.0	32.0	39.0	14.0	40.0
40	32.21507891143898	35.0	31.0	39.0	12.0	40.0
41	31.917052508617484	35.0	31.0	38.0	12.0	40.0
42	32.087295545366274	35.0	32.0	39.0	10.0	40.0
43	31.84746367677635	35.0	31.0	38.0	10.0	40.0
44	31.69942516829365	35.0	31.0	38.0	10.0	40.0
45	31.604913650443418	35.0	31.0	38.0	10.0	40.0
46	31.45346558872202	35.0	30.0	38.0	10.0	40.0
47	31.436762272270048	35.0	31.0	38.0	10.0	40.0
48	31.503620466305076	35.0	31.0	38.0	10.0	40.0
49	31.583315653243943	35.0	31.0	38.0	10.0	40.0
50	31.699959314994295	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	1.0
13	5.0
14	19.0
15	50.0
16	120.0
17	292.0
18	544.0
19	899.0
20	1330.0
21	2002.0
22	2851.0
23	3890.0
24	5715.0
25	8688.0
26	12430.0
27	14444.0
28	13326.0
29	12904.0
30	13680.0
31	16511.0
32	22086.0
33	34854.0
34	57419.0
35	36486.0
36	34009.0
37	46963.0
38	49732.0
39	9384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.87007505509948	21.568544250559732	27.122671532227265	24.438709162113522
2	38.43934314931896	19.74695424060064	29.157421019920676	12.65628159015972
3	26.52911973122936	19.21655155888468	41.314749687374416	12.939579022511538
4	23.709374274596332	24.674582354688386	37.412483557516865	14.203559813198416
5	20.083167140493064	27.85699844498414	38.77605525173535	13.283779162787447
6	17.496798863815055	35.76910884861434	36.45251710392648	10.28157518364413
7	73.22003100047674	5.310017247447203	18.708363389485296	2.761588362590761
8	74.70291209792357	4.105191955850529	18.130536467992382	3.0613594782335216
9	71.75337398505887	4.885695102074436	19.425717416427258	3.935213496439438
10	48.24942154907535	17.621599494807047	23.92003773971081	10.20894121640679
11	43.394177800962964	17.145110685679626	28.579094895903793	10.881616617453618
12	38.94703211619438	17.99999500797476	30.78357324174631	12.26939963408455
13	20.054462995364904	35.818529898487164	31.94047509104206	12.186532015105868
14	14.15488756711154	37.545521030154326	34.80265276221237	13.496938640521766
15	12.330801544532608	19.964856142312655	54.517408440017064	13.186933873137663
16	14.658582913795213	15.539924969860646	52.78517568184825	17.01631643449589
17	15.361709668804085	15.93254775496145	36.72932490346671	31.976417672767752
18	19.836311492390905	19.874250884212472	41.63324089766598	18.656196725730645
19	25.776571926347657	21.239569787264845	34.52859057655395	18.455267709833542
20	27.57320181010835	19.59319986321851	34.04860734975876	18.78499097691438
21	19.961112123382897	25.501012133117346	36.04666545194053	18.491210291559234
22	19.947134452711794	21.31644697595591	34.79541432561483	23.94100424571747
23	17.029545301381045	26.35989007560422	33.62977643214964	22.98078819086509
24	17.87394637067285	20.301318643467063	45.90741290787966	15.917322077980426
25	15.665224803376606	21.302219704022825	43.48653026789703	19.546025224703538
26	14.481366017786588	29.030873180094797	36.76277147257257	19.724989329546048
27	15.387168997526452	29.775683345854997	37.0223567850359	17.814790871582648
28	13.608760005890591	25.017784089916358	44.897026999368514	16.47642890482454
29	14.779639525857444	21.48442862527113	44.31196164127806	19.42397020759337
30	17.38123347951647	26.352402037744703	39.09928888600461	17.167075596734215
31	26.43776566934322	23.065652619939648	33.135565933421354	17.361015777295773
32	27.727954592538417	22.851245135895407	33.328258107672994	16.09254216389318
33	26.287505709628867	23.85414300654704	31.79870157423516	18.059649709588932
34	18.54412575909984	25.198245802330778	33.9233075162428	22.334320922326583
35	19.19833066675985	23.462019423970208	36.314238004787356	21.02541190448259
36	28.086631606009398	23.050426942958623	31.10456046465771	17.758380986374267
37	19.702026013443525	29.249274284330784	32.71349019940645	18.335209502819247
38	20.02525964771278	30.72891056537182	28.67993380574532	20.565895981170083
39	19.679312298602984	29.167405070400036	31.203901766927334	19.94938086406965
40	23.355190083841062	23.963218758034042	30.078449676641565	22.60314148148333
41	17.611615444327686	22.81006092766805	31.5253881923627	28.05293543564156
42	21.666637546519436	23.181966808024182	28.52617942836319	26.625216217093197
43	21.116266763844756	24.201837564490727	29.511355609413965	25.170540062250556
44	19.34260019618659	28.42359330968777	30.419654601773665	21.81415189235197
45	17.133129825104394	33.752081050521795	27.029570261507242	22.085218862866572
46	21.661645521279755	30.214482364422835	28.82969456293571	19.2941775513617
47	20.39791433185486	26.24931671654532	30.2212215984964	23.131547353103418
48	21.857582511937178	22.59814945624365	33.474524447195606	22.069743584623563
49	20.43859933755825	22.37550513055394	35.01631144247065	22.169584089417153
50	18.803960672825163	28.982450535269905	30.99623351695666	21.21735527494827
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	271.5
2	424.0
3	6082.5
4	11741.0
5	8371.5
6	5002.0
7	5196.5
8	5391.0
9	5829.0
10	6267.0
11	6240.0
12	6213.0
13	5877.5
14	5542.0
15	5103.0
16	4664.0
17	4355.5
18	4047.0
19	3661.0
20	3275.0
21	2949.5
22	2624.0
23	2590.5
24	2557.0
25	2496.5
26	2436.0
27	2604.0
28	2772.0
29	3314.0
30	3856.0
31	4586.5
32	5317.0
33	5780.5
34	6244.0
35	7156.0
36	8068.0
37	9125.5
38	10183.0
39	13722.5
40	17262.0
41	23435.5
42	29609.0
43	35552.5
44	41496.0
45	41233.5
46	40971.0
47	37853.5
48	34736.0
49	31875.5
50	29015.0
51	26897.0
52	24779.0
53	22376.5
54	19974.0
55	17723.0
56	15472.0
57	14168.5
58	12865.0
59	11787.5
60	10710.0
61	9556.5
62	8403.0
63	7251.0
64	6099.0
65	5146.5
66	4194.0
67	3560.0
68	2926.0
69	2487.0
70	2048.0
71	1736.5
72	1425.0
73	1110.5
74	796.0
75	613.5
76	431.0
77	357.0
78	283.0
79	231.0
80	179.0
81	159.0
82	139.0
83	91.0
84	43.0
85	36.0
86	29.0
87	17.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	400639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.34747941678889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28852376367277	25.859710202931712
2	8.039675875673801	5.522852033147201
3	3.884417255246131	4.002598251623582
4	2.3241517719262488	3.19315020590921
5	1.5361308672601461	2.638111167235597
6	1.110993364713544	2.289589303601249
7	0.8401498573915089	2.019992095364108
8	0.6519035544520175	1.7912995134617753
9	0.5170837992398408	1.5984472636030886
>10	5.401342470384039	39.53771702837918
>50	0.3815956339334707	8.2500605646774
>100	0.022575314221254944	1.2691631235840624
>500	7.282359426211272E-4	0.16308521020433753
>1k	0.0	0.0
>5k	7.282359426211272E-4	1.8642240362774964
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7453	1.8602782055666074	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	652	0.16274002281355535	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	480	0.11980860575231068	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	448	0.1118213653688233	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATG	401	0.10009010605557622	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03843859434553301	0.0
2	0.0	0.0	0.0	0.1657352379573631	0.0
3	0.0	0.0	0.0	0.2124106739483675	0.0
4	0.0	0.0	0.0	0.333966488534566	0.0
5	0.0	0.0	0.0	0.6651873631873083	0.0
6	0.0	0.0	0.0	0.9020589608101058	0.0
7	0.0	0.0	0.0	1.0862646921542836	0.0
8	0.0	0.0	0.0	1.6136721587264344	0.0
9	0.0	0.0	0.0	1.8370652882020972	0.0
10	0.0	0.0	0.0	2.2841011484154063	0.0
11	0.0	0.0	0.0	2.517727929632412	0.0
12	0.0	0.0	0.0	2.840961563901667	0.0
13	0.0	0.0	0.0	2.9510357204366024	0.0
14	0.0	0.0	0.0	2.9922199286639595	0.0
15	0.0	0.0	0.0	3.0506266239682107	0.0
16	0.0	0.0	0.0	3.168188818362666	0.0
17	0.0	0.0	0.0	3.2992294809042555	0.0
18	0.0	0.0	0.0	3.5196273952361103	0.0
19	4.992025239679612E-4	0.0	0.0	3.6084854445024073	0.0
20	4.992025239679612E-4	0.0	0.0	3.6993403038645765	0.0
21	4.992025239679612E-4	0.0	0.0	3.8016768212780083	0.0
22	4.992025239679612E-4	0.0	0.0	3.9040133386914406	0.0
23	4.992025239679612E-4	0.0	0.0	4.0165835078462155	0.0
24	4.992025239679612E-4	0.0	0.0	4.103943949540609	0.0
25	4.992025239679612E-4	0.0	0.0	4.178574726873819	0.0
26	4.992025239679612E-4	0.0	0.0	4.2557015168268695	0.0
27	7.488037859519418E-4	0.0	0.0	4.338818737067535	0.0
28	7.488037859519418E-4	0.0	0.0	4.412201508090825	0.0
29	7.488037859519418E-4	0.0	0.0	4.49407072202157	0.0
30	7.488037859519418E-4	0.0	0.0	4.60439447981849	0.0
31	7.488037859519418E-4	0.0	0.0	4.697745351800498	0.0
32	7.488037859519418E-4	0.0	0.0	4.77811695815934	0.0
33	7.488037859519418E-4	0.0	0.0	4.867723811211589	0.0
34	7.488037859519418E-4	0.0	0.0	4.955833056691935	0.0
35	7.488037859519418E-4	0.0	0.0	5.0751424599202775	0.0
36	9.984050479359223E-4	0.0	0.0	5.1767301735477576	0.0
37	9.984050479359223E-4	0.0	0.0	5.27657067834135	0.0
38	9.984050479359223E-4	0.0	0.0	5.395880081569692	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGG	40	8.289135E-9	44.0	2
CGTATGG	20	7.8503345E-4	44.0	2
CGAAAGT	20	7.8503345E-4	44.0	30
GCGAAAG	25	4.437632E-5	44.0	1
TTTCGAG	25	4.437632E-5	44.0	1
CTATACG	20	7.8503345E-4	44.0	1
CGACAGG	45	4.783942E-10	44.0	2
ATACTCC	20	7.8503345E-4	44.0	44
ATACTAG	20	7.8503345E-4	44.0	1
CTAAACG	20	7.8503345E-4	44.0	16
GTCGTCT	20	7.8503345E-4	44.0	12
ACTAACG	25	4.437632E-5	44.0	1
CGGTCTA	40	8.289135E-9	44.0	31
TATACGG	20	7.8503345E-4	44.0	2
TCGACCC	20	7.8503345E-4	44.0	29
TTCGAAG	25	4.437632E-5	44.0	1
ACGACTG	25	4.437632E-5	44.0	1
TACGTAG	35	1.4429315E-7	43.999996	1
CGTTTTT	5565	0.0	43.644203	1
GCATAGG	90	0.0	41.555557	2
>>END_MODULE