FastQCFastQC Report
Thu 26 May 2016
SRR1545995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545995_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences393721
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74671.896520632630721No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA6170.15670995451093542No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC4770.12115178006761133No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT4690.11911988438513567No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.8501686E-444.06
TCGTTCA207.8501686E-444.016
TAGCCTT207.8501686E-444.038
TCGATAG207.8501686E-444.01
TGGTTAG207.8501686E-444.01
CCCGTGC207.8501686E-444.033
CGGATGC207.8501686E-444.015
ACTACGG207.8501686E-444.02
GCGATAT454.783942E-1044.09
TACTCGG254.4374923E-544.02
TATAGCG207.8501686E-444.01
AACTCGA207.8501686E-444.013
ATGTTAG302.5234604E-644.01
TGTCGGG302.5234604E-644.03
AATACGG207.8501686E-444.02
ACCTTAT254.4374923E-544.039
TACACAA750.044.030
ACGGTCT454.783942E-1044.030
CGTTTTT53400.043.711611
GCGTAGG950.041.684212
Produced by FastQC (version 0.11.5)