##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545995_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	393721
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.88586587964574	31.0	28.0	31.0	23.0	33.0
2	28.685101886869127	31.0	28.0	33.0	16.0	33.0
3	28.05667973006266	31.0	26.0	33.0	16.0	34.0
4	32.004139987453044	35.0	32.0	35.0	25.0	37.0
5	33.59314844775869	35.0	33.0	35.0	28.0	37.0
6	33.43404847595124	35.0	33.0	37.0	28.0	37.0
7	34.2341632780573	35.0	33.0	37.0	31.0	37.0
8	34.651540049933836	35.0	35.0	37.0	32.0	37.0
9	36.550585312950034	39.0	37.0	39.0	32.0	39.0
10	36.3877999903485	39.0	35.0	39.0	32.0	39.0
11	36.37824246103205	38.0	35.0	39.0	32.0	39.0
12	35.10573985131603	37.0	34.0	39.0	30.0	39.0
13	34.824807414387344	37.0	33.0	39.0	27.0	39.0
14	35.553678874126604	38.0	33.0	40.0	27.0	41.0
15	36.18016819016512	38.0	34.0	40.0	30.0	41.0
16	35.900284719382505	37.0	34.0	40.0	30.0	41.0
17	35.47159536829379	36.0	33.0	40.0	29.0	41.0
18	35.10190464821536	36.0	33.0	39.0	29.0	40.0
19	34.50092832233993	36.0	33.0	39.0	27.0	40.0
20	34.960311997582046	35.0	34.0	39.0	29.0	40.0
21	35.0505611841888	35.0	34.0	39.0	30.0	40.0
22	35.430378364374775	35.0	34.0	39.0	30.0	40.0
23	35.805743153146516	35.0	34.0	40.0	31.0	41.0
24	35.39436555327249	35.0	34.0	39.0	31.0	41.0
25	35.06580040180737	35.0	34.0	39.0	30.0	41.0
26	35.14934433266196	35.0	34.0	39.0	30.0	41.0
27	35.478041557346444	35.0	34.0	40.0	31.0	41.0
28	35.4290042949195	35.0	34.0	40.0	31.0	41.0
29	35.586519388094615	36.0	35.0	40.0	31.0	41.0
30	35.25291259546735	36.0	34.0	40.0	30.0	41.0
31	34.514643618196644	35.0	34.0	39.0	27.0	40.0
32	34.39739307783938	35.0	34.0	39.0	26.0	40.0
33	34.03358215589212	35.0	33.0	40.0	23.0	41.0
34	33.57406640743064	35.0	33.0	40.0	20.0	41.0
35	33.230767472397964	35.0	33.0	40.0	18.0	41.0
36	32.831835233579106	35.0	33.0	40.0	15.0	41.0
37	32.73263300662144	35.0	33.0	40.0	15.0	41.0
38	32.59596008340932	35.0	32.0	39.0	15.0	41.0
39	32.39536880176572	35.0	32.0	39.0	14.0	40.0
40	32.24731218299252	35.0	31.0	39.0	12.0	40.0
41	31.93645754226978	35.0	31.0	39.0	11.0	40.0
42	32.13719867621996	35.0	32.0	39.0	10.0	40.0
43	31.902697595505447	35.0	31.0	39.0	10.0	40.0
44	31.749896500313675	35.0	31.0	39.0	10.0	40.0
45	31.668643531841074	35.0	31.0	38.0	10.0	40.0
46	31.517531449935362	35.0	31.0	38.0	10.0	40.0
47	31.50882985667516	35.0	31.0	38.0	10.0	40.0
48	31.56466635002959	35.0	31.0	38.0	10.0	40.0
49	31.662075429047473	35.0	31.0	38.0	10.0	40.0
50	31.70333307088014	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	9.0
14	23.0
15	40.0
16	120.0
17	291.0
18	514.0
19	889.0
20	1441.0
21	1978.0
22	2836.0
23	3915.0
24	5749.0
25	8628.0
26	12177.0
27	14148.0
28	13020.0
29	12562.0
30	13558.0
31	16193.0
32	21702.0
33	34372.0
34	55554.0
35	35873.0
36	32985.0
37	45721.0
38	48974.0
39	10447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.641454227739946	21.65721411862715	27.12809324369287	24.573238409940036
2	38.44067245587611	19.864828139723308	29.095730225210236	12.598769179190342
3	26.650851745271396	19.21233563868831	41.20430457100333	12.932508045036966
4	23.766321837036884	24.69693005961074	37.187246806748945	14.349501296603432
5	20.05430241211416	28.076480553488388	38.53007586590505	13.33914116849241
6	17.498177643559778	35.796160225134045	36.32115127209369	10.384510859212488
7	73.008043767033	5.410430228512069	18.749825383964787	2.8317006204901443
8	74.60816161698258	4.1669100708369635	18.141018640103017	3.0839096720774357
9	71.59359038506963	5.0096388051437435	19.47114835124365	3.925622458542979
10	48.18894597951341	18.051107256153472	23.894077278072544	9.865869486260575
11	43.21994508801918	17.248508461575582	28.633473957447027	10.898072492958212
12	38.79041250022224	18.287061142280955	30.921896469835236	12.000629887661567
13	19.975058480497612	35.796160225134045	31.93428849362874	12.294492800739611
14	14.154185324125459	37.473236124057394	34.80053134072097	13.572047211096184
15	12.386690067331942	19.845779117700097	54.485282725584874	13.282248089383092
16	14.444238432798858	15.712141338663674	52.76426708252798	17.079353146009485
17	15.387038029467567	15.982637451393245	36.67901889916972	31.95130561996947
18	19.899624353285702	19.907751936015604	41.61144566837939	18.58117804231931
19	25.80304327175843	21.375288592683653	34.37408723436139	18.447580901196535
20	27.55453735005245	19.627096344873653	34.176739366200934	18.641626938872957
21	19.885147096548064	25.635411877954184	35.87210232626657	18.607338699231182
22	19.954993510633166	21.310775904765052	34.865298015599876	23.868932569001906
23	17.079607132969794	26.50379329525222	33.52526281300718	22.891336758770805
24	17.93884501969669	20.212536288386953	45.819755613746786	16.028863078169568
25	15.609276619738344	21.35217577929549	43.56993911932561	19.468608481640555
26	14.488686150853013	29.21129429215104	36.54237391452323	19.757645642472717
27	15.328113054675773	29.762953969943183	37.07955633557773	17.829376639803314
28	13.738408670098877	24.90646930186604	44.89219523469665	16.462926793338433
29	14.760706185344445	21.53657031248016	44.34282143954729	19.359902062628105
30	17.42680730771282	26.47509276873725	38.94407461120946	17.154025312340465
31	26.60843592289972	23.036109326147194	32.99011228763516	17.365342463317933
32	27.626161672859716	22.90149623718318	33.386839919638525	16.085502170318573
33	26.309747257575793	23.869694529882835	31.772498799911613	18.048059412629755
34	18.574574381351262	25.080958343598642	33.97862953715956	22.365837737890537
35	19.344916831969847	23.39448492714384	36.304388132713264	20.956210108173046
36	27.897419746470213	22.971342651268284	31.379326985352574	17.75191061690893
37	19.564107578716907	29.274537045268094	32.80647971533141	18.354875660683582
38	20.04617482938426	30.75070925858666	28.70230442369089	20.50081148833819
39	19.583918561621047	29.252948153641796	31.246491805110725	19.916641479626435
40	23.519192524655786	24.025896510473153	29.968175433873224	22.48673553099784
41	17.55964248795467	22.76154942205267	31.756497621412116	27.92231046858054
42	21.647054640214773	23.18443771096792	28.567183360806258	26.601324288011053
43	21.185560333332486	24.136126851247457	29.458169617571833	25.220143197848223
44	19.421620893983302	28.353834314146315	30.57495028205252	21.649594509817867
45	17.085956806977528	33.82953919145791	27.120219647923278	21.964284353641283
46	21.77557204213136	30.360585287551338	28.882127191589984	18.981715478727324
47	20.356292907922107	26.205612603848916	30.220892459381137	23.21720202884784
48	21.784461585742186	22.598489793534	33.72591251165165	21.89113610907216
49	20.27120727621844	22.313516424066787	35.387749192956434	22.02752710675834
50	18.760492836297786	29.10842957322571	31.02399922787964	21.107078362596866
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	259.0
2	386.0
3	6071.5
4	11757.0
5	8381.5
6	5006.0
7	5130.5
8	5255.0
9	5714.5
10	6174.0
11	6197.5
12	6221.0
13	5852.0
14	5483.0
15	5006.5
16	4530.0
17	4199.0
18	3868.0
19	3453.5
20	3039.0
21	2828.5
22	2618.0
23	2535.5
24	2453.0
25	2387.5
26	2322.0
27	2514.0
28	2706.0
29	3225.0
30	3744.0
31	4544.5
32	5345.0
33	5719.5
34	6094.0
35	7121.5
36	8149.0
37	9093.5
38	10038.0
39	13631.0
40	17224.0
41	23279.5
42	29335.0
43	35064.5
44	40794.0
45	40636.0
46	40478.0
47	37243.5
48	34009.0
49	31337.0
50	28665.0
51	26382.0
52	24099.0
53	21681.0
54	19263.0
55	17221.0
56	15179.0
57	13918.0
58	12657.0
59	11468.0
60	10279.0
61	9314.0
62	8349.0
63	7108.5
64	5868.0
65	4968.5
66	4069.0
67	3465.0
68	2861.0
69	2393.5
70	1926.0
71	1670.0
72	1414.0
73	1106.0
74	798.0
75	618.5
76	439.0
77	357.5
78	276.0
79	230.5
80	185.0
81	172.0
82	159.0
83	96.0
84	33.0
85	25.0
86	17.0
87	12.5
88	8.0
89	8.5
90	9.0
91	6.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	393721.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.16339481866969
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.97638369558572	25.614477982683642
2	8.072787233893456	5.51587635117237
3	3.923398542663014	4.021098403319694
4	2.264972846402335	3.095166464208363
5	1.605973767005323	2.743275793531455
6	1.1624988707233601	2.3828944738067883
7	0.8579385441764462	2.0517065250398234
8	0.7096698984342703	1.9395786344908155
9	0.5831564881104049	1.793034480994616
>10	5.44603651539646	39.586857234096186
>50	0.37115096480712206	7.889413119892151
>100	0.024545053784839736	1.3124458504329737
>500	7.437895086315072E-4	0.15678201156188667
>1k	0.0	0.0
>5k	7.437895086315072E-4	1.8973926747692185
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7467	1.896520632630721	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	617	0.15670995451093542	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	477	0.12115178006761133	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	469	0.11911988438513567	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0396219658082754	0.0
2	0.0	0.0	0.0	0.16763139380424208	0.0
3	0.0	0.0	0.0	0.21868277282644308	0.0
4	0.0	0.0	0.0	0.3433903703383868	0.0
5	0.0	0.0	0.0	0.6578262272014955	0.0
6	0.0	0.0	0.0	0.8988598525351709	0.0
7	0.0	0.0	0.0	1.0890960858069547	0.0
8	0.0	0.0	0.0	1.5912283063387527	0.0
9	2.539869603094577E-4	0.0	0.0	1.8248963098234536	0.0
10	2.539869603094577E-4	0.0	0.0	2.278517020936145	0.0
11	2.539869603094577E-4	0.0	0.0	2.509137180897133	0.0
12	5.079739206189154E-4	0.0	0.0	2.836018398815405	0.0
13	7.619608809283731E-4	0.0	0.0	2.944978804788162	0.0
14	7.619608809283731E-4	0.0	0.0	2.9802829922711767	0.0
15	7.619608809283731E-4	0.0	0.0	3.043525745388232	0.0
16	7.619608809283731E-4	0.0	0.0	3.148422359996038	0.0
17	7.619608809283731E-4	0.0	0.0	3.2642404138971504	0.0
18	7.619608809283731E-4	0.0	0.0	3.469715864787502	0.0
19	7.619608809283731E-4	0.0	0.0	3.5601352226576686	0.0
20	7.619608809283731E-4	0.0	0.0	3.655126345813406	0.0
21	7.619608809283731E-4	0.0	0.0	3.7493555080882146	0.0
22	7.619608809283731E-4	0.0	0.0	3.847648461727975	0.0
23	7.619608809283731E-4	0.0	0.0	3.9530530502563996	0.0
24	0.0010159478412378308	0.0	0.0	4.032296981872951	0.0
25	0.0010159478412378308	0.0	0.0	4.105191239481765	0.0
26	0.0010159478412378308	0.0	0.0	4.178847457971508	0.0
27	0.0010159478412378308	0.0	0.0	4.245138054612276	0.0
28	0.0010159478412378308	0.0	0.0	4.317016364379853	0.0
29	0.0012699348015472885	0.0	0.0	4.386862778464954	0.0
30	0.0012699348015472885	0.0	0.0	4.489981484350594	0.0
31	0.0012699348015472885	0.0	0.0	4.5870045031888065	0.0
32	0.0012699348015472885	0.0	0.0	4.668026343527523	0.0
33	0.0012699348015472885	0.0	0.0	4.761493544921404	0.0
34	0.0012699348015472885	0.0	0.0	4.852674863672499	0.0
35	0.0012699348015472885	0.0	0.0	4.976366513343205	0.0
36	0.0015239217618567462	0.0	0.0	5.066531884253062	0.0
37	0.0015239217618567462	0.0	0.0	5.155935294281991	0.0
38	0.0015239217618567462	0.0	0.0	5.253720274001132	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.8501686E-4	44.0	6
TCGTTCA	20	7.8501686E-4	44.0	16
TAGCCTT	20	7.8501686E-4	44.0	38
TCGATAG	20	7.8501686E-4	44.0	1
TGGTTAG	20	7.8501686E-4	44.0	1
CCCGTGC	20	7.8501686E-4	44.0	33
CGGATGC	20	7.8501686E-4	44.0	15
ACTACGG	20	7.8501686E-4	44.0	2
GCGATAT	45	4.783942E-10	44.0	9
TACTCGG	25	4.4374923E-5	44.0	2
TATAGCG	20	7.8501686E-4	44.0	1
AACTCGA	20	7.8501686E-4	44.0	13
ATGTTAG	30	2.5234604E-6	44.0	1
TGTCGGG	30	2.5234604E-6	44.0	3
AATACGG	20	7.8501686E-4	44.0	2
ACCTTAT	25	4.4374923E-5	44.0	39
TACACAA	75	0.0	44.0	30
ACGGTCT	45	4.783942E-10	44.0	30
CGTTTTT	5340	0.0	43.71161	1
GCGTAGG	95	0.0	41.68421	2
>>END_MODULE