FastQCFastQC Report
Thu 26 May 2016
SRR1545994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545994_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences336446
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37511.1148891649774406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGT302.522358E-644.00000433
TCGTTCA408.281859E-944.016
TCGTTAG254.436107E-544.01
TCGCAAT351.4420038E-744.021
AGTCACG207.848533E-444.01
CCGATTC207.848533E-444.09
CGACGGT351.4420038E-744.028
ACTGACG351.4420038E-744.026
TCACATC254.436107E-544.017
GCGAAAG254.436107E-544.01
AGGGTCC351.4420038E-744.06
ATATGAG254.436107E-544.01
AAGCGAC254.436107E-544.015
GACCCCG207.848533E-444.043
ATGTACG207.848533E-444.01
AGTGCTA254.436107E-544.025
TATCTCG207.848533E-444.025
AGGTTAG254.436107E-544.01
TAGGGAC800.044.05
CGTGTTT502.7284841E-1144.01
Produced by FastQC (version 0.11.5)