##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545994_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	336446
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.91879826183102	28.0	25.0	30.0	16.0	33.0
2	26.1639757940353	30.0	25.0	31.0	10.0	33.0
3	26.313824506755914	30.0	25.0	31.0	16.0	33.0
4	27.945792192506374	32.0	19.0	35.0	10.0	37.0
5	31.18534326459521	33.0	30.0	35.0	19.0	37.0
6	29.787778722291243	33.0	28.0	35.0	17.0	36.0
7	29.702540675175214	32.0	28.0	35.0	17.0	35.0
8	32.31989383140237	33.0	32.0	35.0	28.0	35.0
9	26.956709249032535	30.0	23.0	34.0	10.0	35.0
10	31.629369943467896	34.0	30.0	37.0	17.0	37.0
11	33.62012626097501	35.0	32.0	37.0	27.0	39.0
12	32.93656931572971	35.0	31.0	37.0	23.0	39.0
13	32.5015188172842	35.0	30.0	37.0	22.0	39.0
14	32.263843231900516	35.0	29.0	38.0	20.0	40.0
15	31.451888267359397	33.0	27.0	38.0	17.0	40.0
16	30.263299311033567	33.0	26.0	37.0	17.0	40.0
17	30.309954643538635	32.0	25.0	37.0	17.0	40.0
18	31.26491621240853	34.0	27.0	37.0	18.0	39.0
19	30.909313827479	34.0	27.0	37.0	18.0	39.0
20	31.55951029288504	34.0	29.0	36.0	19.0	39.0
21	32.33445783275771	34.0	31.0	37.0	23.0	39.0
22	32.70360771119288	34.0	31.0	37.0	24.0	39.0
23	34.0234480421821	35.0	33.0	38.0	28.0	39.0
24	33.67115971062221	35.0	33.0	38.0	26.0	40.0
25	32.5054094862177	34.0	31.0	37.0	23.0	39.0
26	26.136336886157064	33.0	19.0	35.0	0.0	38.0
27	29.28857231175285	33.0	23.0	36.0	12.0	39.0
28	30.788530700320408	33.0	27.0	36.0	18.0	39.0
29	32.14056044655012	34.0	30.0	37.0	23.0	39.0
30	31.883639573661153	34.0	30.0	37.0	21.0	39.0
31	30.800865517794833	33.0	29.0	36.0	18.0	38.0
32	30.933383068902586	34.0	29.0	36.0	18.0	38.0
33	30.640072998341488	33.0	29.0	36.0	17.0	38.0
34	30.6033390202291	34.0	29.0	37.0	15.0	39.0
35	29.812320550697585	34.0	27.0	36.0	10.0	39.0
36	29.461134922097454	33.0	25.0	36.0	10.0	39.0
37	29.46821778234843	33.0	26.0	36.0	10.0	39.0
38	29.211944858907522	33.0	26.0	36.0	10.0	38.0
39	29.06016121457827	33.0	24.0	36.0	9.0	38.0
40	29.06024740968833	33.0	25.0	36.0	10.0	38.0
41	28.481557218691854	33.0	23.0	35.0	9.0	38.0
42	28.661119466422548	33.0	24.0	35.0	8.0	38.0
43	28.178706241120416	32.0	23.0	35.0	8.0	38.0
44	27.988934331215113	32.0	23.0	35.0	8.0	38.0
45	27.40874018416031	31.0	23.0	34.0	8.0	37.0
46	26.975330365051153	31.0	22.0	34.0	7.0	38.0
47	27.19983295982119	31.0	23.0	34.0	8.0	37.0
48	27.393126980258348	31.0	23.0	34.0	8.0	37.0
49	27.25243575492055	31.0	23.0	34.0	8.0	37.0
50	28.955639240769692	33.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	1.0
12	17.0
13	49.0
14	154.0
15	333.0
16	635.0
17	1112.0
18	1941.0
19	2791.0
20	4076.0
21	5571.0
22	7380.0
23	9855.0
24	12328.0
25	15639.0
26	18177.0
27	19671.0
28	21806.0
29	24942.0
30	29507.0
31	34239.0
32	35582.0
33	33472.0
34	26207.0
35	19536.0
36	9377.0
37	1997.0
38	45.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.791199776487165	22.3269113022595	27.070020151822284	25.81186876943105
2	38.410324390838355	17.00540354172735	31.0043810893873	13.579890978046997
3	27.809217526735345	14.992599109515345	43.19058630508314	14.007597058666175
4	25.026601594312314	24.381327166915344	36.39960053024854	14.192470708523805
5	19.384685803962597	28.188476011009193	39.556422130148675	12.870416054879536
6	17.359695166534898	34.36836817795426	37.722546857445174	10.549389798065663
7	75.49621633189278	3.7705902284467645	18.7949923613299	1.9382010783305494
8	77.95574921384116	2.6509454711900275	17.593610861772767	1.7996944531960553
9	66.41095450681536	2.908936352341832	17.42775958103232	13.252349559810488
10	50.31951635626519	17.013725828216117	23.881098304036904	8.785659511481784
11	45.396289449124076	16.23499759248141	28.579623475981286	9.789089482413225
12	40.3039417915505	17.889052032124024	30.916699856737782	10.89030631958769
13	19.497036671560963	37.211320687420866	31.840770881508472	11.450871759509699
14	13.230949394553656	38.2513092739994	35.44788762535444	13.069853706092507
15	10.71672720139339	18.059956129661224	55.08848373884665	16.13483293009874
16	12.681975710812434	13.513907135171765	53.397870683556945	20.406246470458854
17	14.342569089839083	14.509906493166808	37.73176081748632	33.4157635995078
18	19.197137133447864	19.08300291874476	43.22446990007312	18.495390047734258
19	26.69611170886264	20.228803433537625	35.1780077635044	17.897077094095337
20	28.379888600250858	18.793506238742623	34.92120578042241	17.905399380584104
21	19.55945382022672	25.37554317780565	36.728033622037415	18.336969379930213
22	19.42600001188898	20.71625164216546	35.166713231841065	24.691035114104494
23	16.14166909400023	26.540366061715698	34.182900079061724	23.135064765222353
24	16.91772230907783	19.33623820761727	48.52754974052299	15.218489742781902
25	14.658518751894805	19.996968309921947	45.973499462023625	19.37101347615962
26	11.24727296505234	41.18759028194718	30.57964725394268	16.9854894990578
27	14.119056252712173	31.264155317643844	37.54064545276211	17.07614297688188
28	12.65403660617157	24.805763777842508	46.80870035607497	15.731499259910953
29	13.745445034269988	21.077973879909408	45.89889610814217	19.27768497767844
30	16.573536317863788	26.926460709891035	39.72851512575569	16.77148784648948
31	27.532798725501266	22.654452720496007	33.465697318440405	16.34705123556232
32	28.4018832145426	22.077242707596465	33.86100592665688	15.659868151204057
33	26.995714022458284	23.337771886127342	32.11570356015527	17.550810531259103
34	18.116726012495317	24.33496014219221	34.00189034793102	23.546423497381454
35	18.942415721988077	22.73173109503457	36.69117778187287	21.634675401104488
36	28.72942463277911	22.155115531169937	31.079281667786212	18.036178168264744
37	18.95995196851798	29.90048923155573	32.232215570998015	18.907343228928266
38	19.327618696611047	31.820559614321464	28.160834130885785	20.6909875581817
39	19.004238421618922	29.811321876318935	30.981494801543192	20.202944900518954
40	23.65045207849105	23.3035910666199	29.701051580342757	23.34490527454629
41	16.998270153308408	21.90306914036725	31.478751419247068	29.619909287077274
42	20.762024217853682	22.149171040820814	27.678735963572166	29.410068777753338
43	20.519786236127047	23.1300119484256	29.38629081635686	26.963910999090494
44	18.408897713154563	27.640691225337793	30.861118871973513	23.08929218953413
45	16.199627874904145	35.14323249496204	26.408398375965238	22.248741254168575
46	20.64551220701093	30.321061923755966	28.574570659184534	20.458855210048565
47	19.611468110781523	25.781551868650542	29.53846976929433	25.068510251273608
48	20.928767172146497	21.818954601927203	34.46229112547036	22.789987100455942
49	19.899181443678927	21.831438031660355	35.20208294941833	23.06729757524239
50	18.31616366370829	29.89692253734626	30.71637053197244	21.070543266973004
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80.0
1	167.0
2	254.0
3	3429.5
4	6605.0
5	5406.5
6	4208.0
7	4149.5
8	4091.0
9	4534.0
10	4977.0
11	5272.5
12	5568.0
13	5531.0
14	5494.0
15	5278.0
16	5062.0
17	4754.0
18	4446.0
19	4095.5
20	3745.0
21	3500.5
22	3256.0
23	3080.0
24	2904.0
25	2702.5
26	2501.0
27	2601.5
28	2702.0
29	3095.5
30	3489.0
31	4115.5
32	4742.0
33	5104.5
34	5467.0
35	5971.0
36	6475.0
37	7261.5
38	8048.0
39	10943.0
40	13838.0
41	18873.5
42	23909.0
43	28307.5
44	32706.0
45	33309.0
46	33912.0
47	31351.5
48	28791.0
49	26586.0
50	24381.0
51	22672.5
52	20964.0
53	18884.0
54	16804.0
55	15000.0
56	13196.0
57	12124.5
58	11053.0
59	10102.0
60	9151.0
61	8172.5
62	7194.0
63	6278.5
64	5363.0
65	4581.5
66	3800.0
67	3189.0
68	2578.0
69	2180.5
70	1783.0
71	1490.0
72	1197.0
73	951.0
74	705.0
75	548.5
76	392.0
77	321.5
78	251.0
79	202.5
80	154.0
81	136.5
82	119.0
83	86.5
84	54.0
85	35.0
86	16.0
87	12.5
88	9.0
89	7.5
90	6.0
91	5.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	336446.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.009851620929844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.20837392561927	42.44446807564687
2	6.513387286716677	6.644938380901371
3	2.586982163675031	3.9588472894516613
4	1.5605492662800575	3.184135460803867
5	0.9884343467921202	2.520994468344838
6	0.7360953052715552	2.252886738045905
7	0.5544400397028875	1.9797332910562717
8	0.4532575631261438	1.8496480832903068
9	0.36712026482960414	1.6854075212395119
>10	2.9449495477121306	29.18295602824997
>50	0.07639170589488588	2.437372413614868
>100	0.009392422855928593	0.660527604685565
>500	0.0	0.0
>1k	6.261615237285728E-4	1.1980846446690303
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3751	1.1148891649774406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027641880123407618	0.0
2	0.0	0.0	0.0	0.12186205215695832	0.0
3	0.0	0.0	0.0	0.16377070911825375	0.0
4	0.0	0.0	0.0	0.24402132883137265	0.0
5	0.0	0.0	0.0	0.43305612193338605	0.0
6	0.0	0.0	0.0	0.5617543379918323	0.0
7	0.0	0.0	0.0	0.6625134494094149	0.0
8	0.0	0.0	0.0	0.9552795991035709	0.0
9	0.0	0.0	0.0	1.1425310451008484	0.0
10	0.0	0.0	0.0	1.4397555625568441	0.0
11	0.0	0.0	0.0	1.604715169744922	0.0
12	0.0	0.0	0.0	1.7993972286785993	0.0
13	0.0	0.0	0.0	1.8680560922109344	0.0
14	0.0	0.0	0.0	1.88915903295031	0.0
15	0.0	0.0	0.0	1.9272037711846774	0.0
16	0.0	0.0	0.0	1.9946737366471885	0.0
17	0.0	0.0	0.0	2.086516112541091	0.0
18	0.0	0.0	0.0	2.2107559608376977	0.0
19	0.0	0.0	0.0	2.2690119662590726	0.0
20	0.0	0.0	0.0	2.331429114924832	0.0
21	0.0	0.0	0.0	2.385821201619279	0.0
22	0.0	0.0	0.0	2.4449688805930223	0.0
23	0.0	0.0	0.0	2.5145194176777252	0.0
24	0.0	0.0	0.0	2.5617781159532287	0.0
25	0.0	0.0	0.0	2.607550691641452	0.0
26	0.0	0.0	0.0	2.6461898789107314	0.0
27	0.0	0.0	0.0	2.7056347824019307	0.0
28	0.0	0.0	0.0	2.754082378747258	0.0
29	0.0	0.0	0.0	2.8087716899591615	0.0
30	0.0	0.0	0.0	2.888725085154824	0.0
31	0.0	0.0	0.0	2.957681173204615	0.0
32	0.0	0.0	0.0	3.0215844444576545	0.0
33	0.0	0.0	0.0	3.0845960421583256	0.0
34	0.0	0.0	0.0	3.1577132734525004	0.0
35	0.0	0.0	0.0	3.2427194854449155	0.0
36	0.0	0.0	0.0	3.3107838999423382	0.0
37	0.0	0.0	0.0	3.3800372125095857	0.0
38	0.0	0.0	0.0	3.4558294644608645	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTCGT	30	2.522358E-6	44.000004	33
TCGTTCA	40	8.281859E-9	44.0	16
TCGTTAG	25	4.436107E-5	44.0	1
TCGCAAT	35	1.4420038E-7	44.0	21
AGTCACG	20	7.848533E-4	44.0	1
CCGATTC	20	7.848533E-4	44.0	9
CGACGGT	35	1.4420038E-7	44.0	28
ACTGACG	35	1.4420038E-7	44.0	26
TCACATC	25	4.436107E-5	44.0	17
GCGAAAG	25	4.436107E-5	44.0	1
AGGGTCC	35	1.4420038E-7	44.0	6
ATATGAG	25	4.436107E-5	44.0	1
AAGCGAC	25	4.436107E-5	44.0	15
GACCCCG	20	7.848533E-4	44.0	43
ATGTACG	20	7.848533E-4	44.0	1
AGTGCTA	25	4.436107E-5	44.0	25
TATCTCG	20	7.848533E-4	44.0	25
AGGTTAG	25	4.436107E-5	44.0	1
TAGGGAC	80	0.0	44.0	5
CGTGTTT	50	2.7284841E-11	44.0	1
>>END_MODULE