FastQCFastQC Report
Thu 26 May 2016
SRR1545993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545993_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences838280
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133691.5948131889106265No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10650.12704585579997135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC650.044.00000425
ACTATCG408.307325E-944.01
CGACGGT600.044.028
TCCGATC207.855261E-444.014
TTGTACG408.307325E-944.01
ATGTACG408.307325E-944.01
ACTAGCG207.855261E-444.01
ATAACGC207.855261E-444.011
GCTAACG207.855261E-444.01
CGTTTTT93700.043.2251851
GCGTAAG900.041.5555571
AGGGTAC1700.041.4117626
GCGAGAC800.041.2521
TCGATAG650.040.6153871
TGCGAAG650.040.6153871
CACAACG603.6379788E-1240.33333212
AATCGAG501.3478711E-939.61
TCTAGCG452.3495886E-839.111111
TCGTAAT452.3495886E-839.1111131
CGTAAGG1350.039.111112
Produced by FastQC (version 0.11.5)