##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545993_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	838280
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.94280312067567	31.0	28.0	33.0	23.0	33.0
2	28.682151548408648	31.0	28.0	33.0	16.0	33.0
3	28.133499546690842	31.0	27.0	33.0	16.0	34.0
4	32.14657035835282	35.0	32.0	35.0	25.0	37.0
5	33.59793028582335	35.0	33.0	35.0	28.0	37.0
6	33.38207758744095	35.0	33.0	37.0	28.0	37.0
7	34.36564274466765	35.0	35.0	37.0	31.0	37.0
8	34.72649949897409	35.0	35.0	37.0	32.0	37.0
9	36.490756071956866	39.0	37.0	39.0	32.0	39.0
10	36.231003960490526	38.0	35.0	39.0	31.0	39.0
11	36.26001932528511	38.0	35.0	39.0	31.0	39.0
12	35.00966144963497	37.0	33.0	39.0	29.0	39.0
13	35.166060266259485	37.0	34.0	39.0	27.0	39.0
14	35.829447201412414	38.0	34.0	40.0	27.0	41.0
15	36.483025957913824	38.0	35.0	40.0	31.0	41.0
16	36.127724626616406	38.0	34.0	40.0	31.0	41.0
17	35.70334375149115	37.0	34.0	40.0	29.0	41.0
18	35.342900940020044	37.0	33.0	39.0	29.0	40.0
19	34.84576990981534	37.0	33.0	39.0	27.0	40.0
20	35.29655842916448	36.0	34.0	39.0	30.0	40.0
21	35.471033544877606	36.0	34.0	39.0	30.0	40.0
22	35.82964999761416	36.0	34.0	40.0	31.0	41.0
23	36.17538531278332	37.0	35.0	40.0	31.0	41.0
24	35.84261106074343	36.0	35.0	40.0	31.0	41.0
25	35.45241923939495	36.0	34.0	40.0	30.0	41.0
26	35.58520780646085	36.0	34.0	40.0	30.0	41.0
27	35.86954597509185	37.0	35.0	40.0	31.0	41.0
28	35.81959011308871	36.0	35.0	40.0	31.0	41.0
29	35.99242019373002	37.0	35.0	40.0	31.0	41.0
30	35.63791573221358	36.0	35.0	40.0	30.0	41.0
31	34.980934771198164	36.0	34.0	40.0	29.0	41.0
32	34.91609008923033	36.0	34.0	40.0	28.0	41.0
33	34.617707687168966	36.0	34.0	40.0	25.0	41.0
34	34.301686787231	36.0	34.0	40.0	23.0	41.0
35	34.027509901226324	36.0	34.0	40.0	21.0	41.0
36	33.61677124588443	36.0	33.0	40.0	18.0	41.0
37	33.51299446485661	36.0	33.0	40.0	18.0	41.0
38	33.354289736126354	36.0	33.0	40.0	18.0	41.0
39	33.16940521066947	36.0	33.0	40.0	18.0	41.0
40	32.98834041131841	35.0	33.0	40.0	17.0	40.0
41	32.6951579424536	35.0	32.0	39.0	15.0	40.0
42	32.85645607672854	35.0	33.0	39.0	15.0	40.0
43	32.62870282006012	35.0	32.0	39.0	15.0	40.0
44	32.369743999618265	35.0	32.0	39.0	13.0	40.0
45	32.340361215822874	35.0	32.0	39.0	14.0	40.0
46	32.20105573316791	35.0	31.0	39.0	14.0	40.0
47	32.183054587965835	35.0	31.0	38.0	14.0	40.0
48	32.21101541251133	35.0	32.0	38.0	14.0	40.0
49	32.20284272558095	35.0	31.0	38.0	14.0	40.0
50	32.35892183995801	35.0	32.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	2.0
12	5.0
13	17.0
14	36.0
15	109.0
16	221.0
17	503.0
18	963.0
19	1605.0
20	2521.0
21	3766.0
22	5298.0
23	7348.0
24	10675.0
25	15769.0
26	22546.0
27	25895.0
28	25401.0
29	24511.0
30	26955.0
31	32960.0
32	43717.0
33	66208.0
34	104229.0
35	77448.0
36	81471.0
37	112630.0
38	120856.0
39	24612.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.65317077826025	22.900701436274275	27.413632676432698	22.032495109032784
2	36.550675192059934	20.583575893496207	29.861979290929046	13.003769623514817
3	28.172567638497874	18.93794436226559	39.674929617788806	13.214558381447727
4	24.711075058453023	25.159135372429258	36.04380397957723	14.085985589540487
5	20.434461039270886	29.562556663644607	36.711599942739895	13.291382354344611
6	18.77833182230281	36.720785417760176	33.9795772295653	10.521305530371713
7	76.70539676480412	5.059407357923367	15.634990695233098	2.600205182039414
8	78.20704299279477	3.6191964498735505	15.268526029488955	2.9052345278427256
9	74.49825833850264	5.101398100873217	16.691558906332013	3.7087846542921223
10	44.86233716657919	23.47783556806795	21.274395190151264	10.385432075201603
11	38.738607625137185	19.71560815002147	29.140382688361882	12.405401536479458
12	34.73374051629527	20.558286014219593	31.065395810469056	13.64257765901608
13	20.45450207567877	33.59152073292933	31.999451257336453	13.954525934055448
14	15.37863243784893	34.5934532614401	35.530371713508615	14.497542587202366
15	14.747697666650762	21.875387698620983	48.39874504938684	14.978169585341414
16	17.2082120532519	18.522331440568784	46.80261964975903	17.46683685642029
17	17.614281624278284	18.171136135897314	35.26518585675431	28.9493963830701
18	21.023405067519203	21.026983824020615	38.993892255570934	18.95571885288925
19	25.8337309729446	22.545688791334637	32.81648136660781	18.804098869112945
20	26.79665505559002	22.323805888247364	32.54664312640168	18.33289592976094
21	21.365176313403637	25.08433936154984	35.10700481939209	18.443479505654437
22	21.468602376294317	22.05527985875841	33.04695328529847	23.429164479648804
23	18.77761607100253	26.39941308393377	32.648160519158274	22.174810325905426
24	18.652717469103404	21.940401775063226	42.14546452259388	17.26141623323949
25	17.709237963448967	23.293887483895595	39.4852555232142	19.51161902944124
26	17.104189530944314	28.87233382640645	34.27327384644749	19.750202796201748
27	17.035477406117288	28.99914109843966	35.38626711838527	18.579114377057785
28	15.93608340888486	25.587989693181274	41.078875793291026	17.39705110464284
29	16.76611633344467	22.477692417807894	40.68437753495252	20.071813713794914
30	19.13095862957484	26.21749296177888	36.73080593596411	17.920742472682157
31	26.399293792050386	24.065944553132603	31.56761463949993	17.967147015317078
32	26.996826835902088	23.6696569165434	31.85725533234719	17.476260915207327
33	25.273178412940783	24.84575559478933	30.28391468244501	19.597151309824877
34	19.78956911771723	25.250155079448394	32.756000381734026	22.20427542110035
35	19.366202223600705	25.21496397385122	33.74958247840817	21.669251324139903
36	27.602352435940258	24.53655103306771	29.066183136899365	18.794913394092667
37	20.300376962351482	29.478575177744908	30.95063701865725	19.27041084124636
38	21.308989836331534	29.771198167676673	27.952951281194828	20.966860714796965
39	21.270219974232955	28.51588967886625	29.175335210192298	21.0385551367085
40	23.762346709929858	25.236436512859665	28.743021424822256	22.258195352388224
41	19.111752636350623	23.738965500787327	30.183828792288974	26.965453070573076
42	22.435582382974662	24.02502743713318	28.024287827456217	25.515102352435942
43	21.619268025003578	25.174881901035455	28.717970129312402	24.487879944648565
44	20.508660590733406	27.9283771532185	29.56458462566207	21.998377630386027
45	18.388247363649377	32.59030395571885	26.807629908861003	22.213818771770768
46	21.623920408455408	29.38660113565873	28.063057689554803	20.92642076633106
47	20.92188767476261	26.232046571551273	29.98377630386029	22.862289449825834
48	21.991458701149973	24.43777735362886	32.09715130982488	21.47361263539629
49	21.153791096053826	23.909791477787852	32.73369279954192	22.202724626616405
50	19.489550031015888	29.564703917545447	29.863291501646227	21.08245454979243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	263.0
1	583.5
2	904.0
3	10865.0
4	20826.0
5	14866.5
6	8907.0
7	9081.5
8	9256.0
9	9696.0
10	10136.0
11	10151.5
12	10167.0
13	9699.0
14	9231.0
15	8731.0
16	8231.0
17	7761.0
18	7291.0
19	6594.5
20	5898.0
21	5726.5
22	5555.0
23	5402.0
24	5249.0
25	5776.0
26	6303.0
27	7331.5
28	8360.0
29	8906.5
30	9453.0
31	11167.5
32	12882.0
33	14263.5
34	15645.0
35	18691.5
36	21738.0
37	24142.5
38	26547.0
39	32321.5
40	38096.0
41	48964.5
42	59833.0
43	67639.5
44	75446.0
45	75583.0
46	75720.0
47	70237.5
48	64755.0
49	63860.0
50	62965.0
51	58861.5
52	54758.0
53	50174.0
54	45590.0
55	41584.5
56	37579.0
57	33543.5
58	29508.0
59	26764.5
60	24021.0
61	21678.0
62	19335.0
63	17608.0
64	15881.0
65	12998.0
66	10115.0
67	8886.5
68	7658.0
69	6578.5
70	5499.0
71	4501.5
72	3504.0
73	2915.0
74	2326.0
75	1745.5
76	1165.0
77	987.5
78	810.0
79	622.0
80	434.0
81	321.0
82	208.0
83	156.5
84	105.0
85	86.0
86	67.0
87	49.5
88	32.0
89	22.5
90	13.0
91	9.5
92	6.0
93	7.0
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	838280.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.143674813216094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05981705971708	22.62578716987601
2	7.251113605590645	4.371504211212224
3	3.5142871225735512	3.1780058466939
4	2.3428020900232394	2.824826574135341
5	1.6744205761363584	2.5236594673806163
6	1.2239178319956083	2.213602867546323
7	0.9436782557231285	1.9912151328174672
8	0.8082381330358527	1.9490613963098904
9	0.6497662677534793	1.7627708771782178
>10	5.699254660397602	36.45064721879281
>50	0.7555210945593573	15.394339750624308
>100	0.07638759834469837	2.983549768222314
>500	0.0	0.0
>1k	3.978520747119705E-4	0.12772250595533258
>5k	0.0	0.0
>10k+	3.978520747119705E-4	1.60330721325525
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13369	1.5948131889106265	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1065	0.12704585579997135	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02827217636112039	0.0
2	0.0	0.0	0.0	0.10139810087321659	0.0
3	0.0	0.0	0.0	0.13790141718757457	0.0
4	1.192918833802548E-4	0.0	0.0	0.21687264398530323	0.0
5	1.192918833802548E-4	0.0	0.0	0.38662499403540584	0.0
6	1.192918833802548E-4	0.0	0.0	0.6000381734026817	0.0
7	1.192918833802548E-4	0.0	0.0	0.7554754974471537	0.0
8	1.192918833802548E-4	0.0	0.0	1.227513479982822	0.0
9	1.192918833802548E-4	0.0	0.0	1.4764756405974138	0.0
10	2.385837667605096E-4	0.0	0.0	1.8302953667032495	0.0
11	3.578756501407644E-4	0.0	0.0	2.038340411318414	0.0
12	3.578756501407644E-4	0.0	0.0	2.236484229613017	0.0
13	3.578756501407644E-4	0.0	0.0	2.322493677530181	0.0
14	3.578756501407644E-4	0.0	0.0	2.351600897074963	0.0
15	3.578756501407644E-4	0.0	0.0	2.38774633773918	0.0
16	3.578756501407644E-4	0.0	0.0	2.47661879085747	0.0
17	3.578756501407644E-4	0.0	0.0	2.586486615450685	0.0
18	3.578756501407644E-4	0.0	0.0	2.7571933005678293	0.0
19	3.578756501407644E-4	0.0	0.0	2.826859760461898	0.0
20	4.771675335210192E-4	0.0	0.0	2.9116762895452593	0.0
21	4.771675335210192E-4	0.0	0.0	3.0048432504652385	0.0
22	4.771675335210192E-4	0.0	0.0	3.105883475688314	0.0
23	4.771675335210192E-4	0.0	0.0	3.2127690031970224	0.0
24	4.771675335210192E-4	0.0	0.0	3.2943646514291167	0.0
25	4.771675335210192E-4	0.0	0.0	3.360094479171637	0.0
26	4.771675335210192E-4	0.0	0.0	3.4341747387507753	0.0
27	4.771675335210192E-4	0.0	0.0	3.5141003006155462	0.0
28	4.771675335210192E-4	0.0	0.0	3.5925943598797536	0.0
29	4.771675335210192E-4	0.0	0.0	3.681705396764804	0.0
30	4.771675335210192E-4	0.0	0.0	3.8116142577659016	0.0
31	4.771675335210192E-4	0.0	0.0	3.910507229088133	0.0
32	4.771675335210192E-4	0.0	0.0	4.005821443908957	0.0
33	4.771675335210192E-4	0.0	0.0	4.100062031779358	0.0
34	4.771675335210192E-4	0.0	0.0	4.2036073865534185	0.0
35	5.964594169012741E-4	0.0	0.0	4.336140668988882	0.0
36	5.964594169012741E-4	0.0	0.0	4.4333635539437894	0.0
37	7.157513002815288E-4	0.0	0.0	4.531182898315598	0.0
38	7.157513002815288E-4	0.0	0.0	4.652741327480078	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	65	0.0	44.000004	25
ACTATCG	40	8.307325E-9	44.0	1
CGACGGT	60	0.0	44.0	28
TCCGATC	20	7.855261E-4	44.0	14
TTGTACG	40	8.307325E-9	44.0	1
ATGTACG	40	8.307325E-9	44.0	1
ACTAGCG	20	7.855261E-4	44.0	1
ATAACGC	20	7.855261E-4	44.0	11
GCTAACG	20	7.855261E-4	44.0	1
CGTTTTT	9370	0.0	43.225185	1
GCGTAAG	90	0.0	41.555557	1
AGGGTAC	170	0.0	41.411762	6
GCGAGAC	80	0.0	41.25	21
TCGATAG	65	0.0	40.615387	1
TGCGAAG	65	0.0	40.615387	1
CACAACG	60	3.6379788E-12	40.333332	12
AATCGAG	50	1.3478711E-9	39.6	1
TCTAGCG	45	2.3495886E-8	39.11111	1
TCGTAAT	45	2.3495886E-8	39.11111	31
CGTAAGG	135	0.0	39.11111	2
>>END_MODULE