FastQCFastQC Report
Thu 26 May 2016
SRR1545992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545992_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences844016
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139881.6573145532786109No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10710.12689332903641637No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.855292E-444.01
CTCACGA750.044.024
GTCGATC207.855292E-444.021
CTATCGG351.4454781E-744.02
CTAAGCG551.8189894E-1244.01
CCGTACT207.855292E-444.025
TCGTACT254.4418317E-544.033
CGTAATC207.855292E-444.032
ACCGTAA302.5269146E-644.017
ATCGATG254.4418317E-544.01
AGTCGTT254.4418317E-544.028
CTTACCG254.4418317E-544.033
CGTTTTT96800.043.2954561
ATAGGGC2700.041.5555574
TGCGAAG750.041.0666661
CGGTCTA750.041.06666631
TACACGG750.041.0666662
GGGCGAT10300.039.7281577
TCGATAG501.3478711E-939.6000021
CGTTGAT1350.039.11111525
Produced by FastQC (version 0.11.5)